Experiment: 30_an_nonmag_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS12660 and AMB_RS12665 are separated by 90 nucleotides AMB_RS12665 and AMB_RS12670 overlap by 4 nucleotides AMB_RS12670 and AMB_RS12675 overlap by 1 nucleotides
AMB_RS12660: AMB_RS12660 - methyl-accepting chemotaxis protein, at 2,698,130 to 2,700,274
_RS12660
AMB_RS12665: AMB_RS12665 - M23 family peptidase, at 2,700,365 to 2,701,456
_RS12665
AMB_RS12670: AMB_RS12670 - protein-L-isoaspartate O-methyltransferase, at 2,701,453 to 2,702,097
_RS12670
AMB_RS12675: AMB_RS12675 - 5'-nucleotidase SurE, at 2,702,097 to 2,702,879
_RS12675
Position (kb)
2700
2701
2702 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2699.608 kb on + strand, within AMB_RS12660 at 2699.612 kb on + strand, within AMB_RS12660 at 2699.612 kb on + strand, within AMB_RS12660 at 2699.613 kb on - strand, within AMB_RS12660 at 2699.806 kb on + strand, within AMB_RS12660 at 2699.807 kb on - strand, within AMB_RS12660 at 2699.808 kb on + strand, within AMB_RS12660 at 2699.808 kb on + strand, within AMB_RS12660 at 2699.809 kb on - strand, within AMB_RS12660 at 2699.809 kb on - strand, within AMB_RS12660 at 2699.946 kb on + strand, within AMB_RS12660 at 2700.010 kb on - strand, within AMB_RS12660 at 2700.096 kb on - strand at 2700.135 kb on + strand at 2700.135 kb on + strand at 2700.136 kb on - strand at 2700.152 kb on - strand at 2700.326 kb on + strand at 2700.327 kb on - strand at 2700.426 kb on + strand at 2700.649 kb on - strand, within AMB_RS12665 at 2700.649 kb on - strand, within AMB_RS12665 at 2700.703 kb on + strand, within AMB_RS12665 at 2700.704 kb on - strand, within AMB_RS12665 at 2701.025 kb on + strand, within AMB_RS12665 at 2701.039 kb on - strand, within AMB_RS12665 at 2701.039 kb on - strand, within AMB_RS12665 at 2701.039 kb on - strand, within AMB_RS12665 at 2701.134 kb on + strand, within AMB_RS12665 at 2701.254 kb on + strand, within AMB_RS12665 at 2701.344 kb on + strand, within AMB_RS12665 at 2701.426 kb on + strand at 2701.959 kb on - strand, within AMB_RS12670 at 2701.959 kb on - strand, within AMB_RS12670 at 2702.246 kb on - strand, within AMB_RS12675
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_nonmag_3 remove 2,699,608 + AMB_RS12660 0.69 -2.1 2,699,612 + AMB_RS12660 0.69 -1.7 2,699,612 + AMB_RS12660 0.69 -0.7 2,699,613 - AMB_RS12660 0.69 -2.2 2,699,806 + AMB_RS12660 0.78 +0.5 2,699,807 - AMB_RS12660 0.78 -1.3 2,699,808 + AMB_RS12660 0.78 -1.4 2,699,808 + AMB_RS12660 0.78 +0.5 2,699,809 - AMB_RS12660 0.78 +1.5 2,699,809 - AMB_RS12660 0.78 -0.5 2,699,946 + AMB_RS12660 0.85 -2.7 2,700,010 - AMB_RS12660 0.88 -0.1 2,700,096 - +0.5 2,700,135 + -0.7 2,700,135 + -0.5 2,700,136 - -1.7 2,700,152 - -1.3 2,700,326 + -0.9 2,700,327 - -0.3 2,700,426 + -1.7 2,700,649 - AMB_RS12665 0.26 -3.5 2,700,649 - AMB_RS12665 0.26 -3.4 2,700,703 + AMB_RS12665 0.31 -0.1 2,700,704 - AMB_RS12665 0.31 -1.5 2,701,025 + AMB_RS12665 0.60 +0.4 2,701,039 - AMB_RS12665 0.62 -0.9 2,701,039 - AMB_RS12665 0.62 -2.4 2,701,039 - AMB_RS12665 0.62 -1.0 2,701,134 + AMB_RS12665 0.70 -0.2 2,701,254 + AMB_RS12665 0.81 +0.1 2,701,344 + AMB_RS12665 0.90 +0.4 2,701,426 + -0.0 2,701,959 - AMB_RS12670 0.78 -0.5 2,701,959 - AMB_RS12670 0.78 -0.3 2,702,246 - AMB_RS12675 0.19 -0.5
Or see this region's nucleotide sequence