Experiment: 30_an_nonmag_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS07465 and AMB_RS07470 are separated by 37 nucleotides AMB_RS07470 and AMB_RS07475 overlap by 4 nucleotides AMB_RS07475 and AMB_RS07480 are separated by 10 nucleotides AMB_RS07480 and AMB_RS07485 overlap by 64 nucleotides
AMB_RS07465: AMB_RS07465 - ABC transporter substrate-binding protein, at 1,615,884 to 1,617,092
_RS07465
AMB_RS07470: AMB_RS07470 - amino acid synthesis family protein, at 1,617,130 to 1,617,714
_RS07470
AMB_RS07475: AMB_RS07475 - amino acid synthesis family protein, at 1,617,711 to 1,618,289
_RS07475
AMB_RS07480: AMB_RS07480 - UPF0280 family protein, at 1,618,300 to 1,619,229
_RS07480
AMB_RS07485: AMB_RS07485 - hypothetical protein, at 1,619,166 to 1,620,647
_RS07485
Position (kb) 1617
1618
1619 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1616.772 kb on - strand, within AMB_RS07465 at 1616.980 kb on + strand at 1616.980 kb on + strand at 1616.980 kb on + strand at 1616.981 kb on - strand at 1616.981 kb on - strand at 1616.981 kb on - strand at 1617.477 kb on - strand, within AMB_RS07470 at 1617.584 kb on + strand, within AMB_RS07470 at 1617.584 kb on + strand, within AMB_RS07470 at 1617.584 kb on + strand, within AMB_RS07470 at 1617.585 kb on - strand, within AMB_RS07470 at 1617.717 kb on + strand at 1617.717 kb on + strand at 1617.717 kb on + strand at 1617.717 kb on + strand at 1617.718 kb on - strand at 1617.867 kb on + strand, within AMB_RS07475 at 1617.867 kb on + strand, within AMB_RS07475 at 1617.867 kb on + strand, within AMB_RS07475 at 1617.868 kb on - strand, within AMB_RS07475 at 1617.868 kb on - strand, within AMB_RS07475 at 1617.871 kb on + strand, within AMB_RS07475 at 1617.871 kb on + strand, within AMB_RS07475 at 1617.871 kb on + strand, within AMB_RS07475 at 1617.871 kb on + strand, within AMB_RS07475 at 1617.871 kb on + strand, within AMB_RS07475 at 1617.871 kb on + strand, within AMB_RS07475 at 1617.872 kb on - strand, within AMB_RS07475 at 1617.872 kb on - strand, within AMB_RS07475 at 1618.163 kb on - strand, within AMB_RS07475 at 1618.163 kb on - strand, within AMB_RS07475 at 1618.782 kb on + strand, within AMB_RS07480 at 1618.782 kb on + strand, within AMB_RS07480 at 1618.782 kb on + strand, within AMB_RS07480 at 1618.783 kb on - strand, within AMB_RS07480 at 1618.817 kb on + strand, within AMB_RS07480 at 1618.817 kb on + strand, within AMB_RS07480 at 1618.817 kb on + strand, within AMB_RS07480 at 1618.817 kb on + strand, within AMB_RS07480 at 1618.817 kb on + strand, within AMB_RS07480 at 1618.817 kb on + strand, within AMB_RS07480 at 1618.818 kb on - strand, within AMB_RS07480 at 1618.818 kb on - strand, within AMB_RS07480 at 1618.818 kb on - strand, within AMB_RS07480 at 1618.818 kb on - strand, within AMB_RS07480 at 1619.054 kb on + strand, within AMB_RS07480 at 1619.054 kb on + strand, within AMB_RS07480 at 1619.054 kb on + strand, within AMB_RS07480 at 1619.055 kb on - strand, within AMB_RS07480 at 1619.055 kb on - strand, within AMB_RS07480 at 1619.055 kb on - strand, within AMB_RS07480 at 1619.055 kb on - strand, within AMB_RS07480 at 1619.055 kb on - strand, within AMB_RS07480 at 1619.055 kb on - strand, within AMB_RS07480 at 1619.188 kb on + strand at 1619.188 kb on + strand at 1619.189 kb on - strand at 1619.189 kb on - strand at 1619.189 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_nonmag_3 remove 1,616,772 - AMB_RS07465 0.73 -3.0 1,616,980 + +0.7 1,616,980 + -2.1 1,616,980 + -1.9 1,616,981 - -1.9 1,616,981 - -2.8 1,616,981 - -0.1 1,617,477 - AMB_RS07470 0.59 -2.4 1,617,584 + AMB_RS07470 0.78 -0.9 1,617,584 + AMB_RS07470 0.78 -0.4 1,617,584 + AMB_RS07470 0.78 +0.2 1,617,585 - AMB_RS07470 0.78 -1.9 1,617,717 + +0.5 1,617,717 + +0.2 1,617,717 + -0.3 1,617,717 + -2.5 1,617,718 - +0.3 1,617,867 + AMB_RS07475 0.27 +1.5 1,617,867 + AMB_RS07475 0.27 +0.1 1,617,867 + AMB_RS07475 0.27 +0.6 1,617,868 - AMB_RS07475 0.27 -0.5 1,617,868 - AMB_RS07475 0.27 -0.4 1,617,871 + AMB_RS07475 0.28 -2.4 1,617,871 + AMB_RS07475 0.28 +0.7 1,617,871 + AMB_RS07475 0.28 +0.6 1,617,871 + AMB_RS07475 0.28 -0.0 1,617,871 + AMB_RS07475 0.28 -0.5 1,617,871 + AMB_RS07475 0.28 -1.5 1,617,872 - AMB_RS07475 0.28 +1.5 1,617,872 - AMB_RS07475 0.28 +0.5 1,618,163 - AMB_RS07475 0.78 -1.9 1,618,163 - AMB_RS07475 0.78 -0.1 1,618,782 + AMB_RS07480 0.52 +0.6 1,618,782 + AMB_RS07480 0.52 -0.5 1,618,782 + AMB_RS07480 0.52 +0.5 1,618,783 - AMB_RS07480 0.52 -1.7 1,618,817 + AMB_RS07480 0.56 -2.1 1,618,817 + AMB_RS07480 0.56 +1.5 1,618,817 + AMB_RS07480 0.56 -0.5 1,618,817 + AMB_RS07480 0.56 -2.2 1,618,817 + AMB_RS07480 0.56 -0.1 1,618,817 + AMB_RS07480 0.56 -1.1 1,618,818 - AMB_RS07480 0.56 -1.4 1,618,818 - AMB_RS07480 0.56 +0.1 1,618,818 - AMB_RS07480 0.56 -1.1 1,618,818 - AMB_RS07480 0.56 +0.2 1,619,054 + AMB_RS07480 0.81 -0.2 1,619,054 + AMB_RS07480 0.81 -1.4 1,619,054 + AMB_RS07480 0.81 -0.2 1,619,055 - AMB_RS07480 0.81 -0.4 1,619,055 - AMB_RS07480 0.81 -1.1 1,619,055 - AMB_RS07480 0.81 +0.2 1,619,055 - AMB_RS07480 0.81 +0.4 1,619,055 - AMB_RS07480 0.81 -0.1 1,619,055 - AMB_RS07480 0.81 -0.1 1,619,188 + -2.9 1,619,188 + +0.0 1,619,189 - -0.4 1,619,189 - +0.5 1,619,189 - -1.4
Or see this region's nucleotide sequence