Experiment: 30_an_nonmag_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01050 and AMB_RS01055 overlap by 1 nucleotides AMB_RS01055 and AMB_RS01060 overlap by 1 nucleotides AMB_RS01060 and AMB_RS01065 overlap by 1 nucleotides
AMB_RS01050: AMB_RS01050 - hydrogenase expression protein HypE, at 231,525 to 233,042
_RS01050
AMB_RS01055: AMB_RS01055 - hydrogenase 4 subunit F, at 233,042 to 234,532
_RS01055
AMB_RS01060: AMB_RS01060 - hydrogenase-4 component E, at 234,532 to 235,194
_RS01060
AMB_RS01065: AMB_RS01065 - formate hydrogenlyase, at 235,194 to 236,141
_RS01065
Position (kb) 233
234
235 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 232.084 kb on + strand, within AMB_RS01050 at 232.084 kb on + strand, within AMB_RS01050 at 232.224 kb on - strand, within AMB_RS01050 at 232.224 kb on - strand, within AMB_RS01050 at 232.486 kb on + strand, within AMB_RS01050 at 233.097 kb on + strand at 233.097 kb on + strand at 233.098 kb on - strand at 233.098 kb on - strand at 233.136 kb on - strand at 233.423 kb on + strand, within AMB_RS01055 at 233.424 kb on - strand, within AMB_RS01055 at 233.743 kb on - strand, within AMB_RS01055 at 233.982 kb on + strand, within AMB_RS01055 at 233.983 kb on - strand, within AMB_RS01055 at 234.040 kb on - strand, within AMB_RS01055 at 234.040 kb on - strand, within AMB_RS01055 at 234.075 kb on + strand, within AMB_RS01055 at 234.139 kb on + strand, within AMB_RS01055 at 234.140 kb on - strand, within AMB_RS01055 at 234.186 kb on + strand, within AMB_RS01055 at 234.186 kb on + strand, within AMB_RS01055 at 234.186 kb on + strand, within AMB_RS01055 at 234.187 kb on - strand, within AMB_RS01055 at 234.187 kb on - strand, within AMB_RS01055 at 234.187 kb on - strand, within AMB_RS01055 at 234.187 kb on - strand, within AMB_RS01055 at 234.187 kb on - strand, within AMB_RS01055 at 234.198 kb on + strand, within AMB_RS01055 at 234.199 kb on - strand, within AMB_RS01055 at 234.199 kb on - strand, within AMB_RS01055 at 234.253 kb on - strand, within AMB_RS01055 at 234.266 kb on + strand, within AMB_RS01055 at 234.309 kb on + strand, within AMB_RS01055 at 234.310 kb on - strand, within AMB_RS01055 at 235.013 kb on + strand, within AMB_RS01060 at 235.013 kb on + strand, within AMB_RS01060 at 235.013 kb on + strand, within AMB_RS01060 at 235.013 kb on + strand, within AMB_RS01060 at 235.013 kb on + strand, within AMB_RS01060 at 235.013 kb on + strand, within AMB_RS01060 at 235.013 kb on + strand, within AMB_RS01060 at 235.013 kb on + strand, within AMB_RS01060 at 235.013 kb on + strand, within AMB_RS01060 at 235.013 kb on + strand, within AMB_RS01060 at 235.014 kb on - strand, within AMB_RS01060 at 235.014 kb on - strand, within AMB_RS01060 at 235.014 kb on - strand, within AMB_RS01060 at 235.014 kb on - strand, within AMB_RS01060 at 235.014 kb on - strand, within AMB_RS01060 at 235.014 kb on - strand, within AMB_RS01060 at 235.118 kb on + strand, within AMB_RS01060 at 235.119 kb on - strand, within AMB_RS01060 at 235.175 kb on + strand at 235.175 kb on + strand at 235.176 kb on - strand at 235.176 kb on - strand at 235.213 kb on + strand at 235.213 kb on + strand at 235.213 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_nonmag_3 remove 232,084 + AMB_RS01050 0.37 +0.5 232,084 + AMB_RS01050 0.37 -1.0 232,224 - AMB_RS01050 0.46 -1.0 232,224 - AMB_RS01050 0.46 +0.0 232,486 + AMB_RS01050 0.63 -2.6 233,097 + +0.1 233,097 + -2.2 233,098 - +0.7 233,098 - -0.9 233,136 - +0.4 233,423 + AMB_RS01055 0.26 +0.7 233,424 - AMB_RS01055 0.26 +0.7 233,743 - AMB_RS01055 0.47 -1.2 233,982 + AMB_RS01055 0.63 +0.7 233,983 - AMB_RS01055 0.63 +1.7 234,040 - AMB_RS01055 0.67 -1.6 234,040 - AMB_RS01055 0.67 +2.3 234,075 + AMB_RS01055 0.69 +0.4 234,139 + AMB_RS01055 0.74 -1.1 234,140 - AMB_RS01055 0.74 +0.1 234,186 + AMB_RS01055 0.77 -2.5 234,186 + AMB_RS01055 0.77 -2.0 234,186 + AMB_RS01055 0.77 +1.1 234,187 - AMB_RS01055 0.77 -1.1 234,187 - AMB_RS01055 0.77 -0.3 234,187 - AMB_RS01055 0.77 +0.1 234,187 - AMB_RS01055 0.77 +0.7 234,187 - AMB_RS01055 0.77 -0.9 234,198 + AMB_RS01055 0.78 +1.7 234,199 - AMB_RS01055 0.78 -1.5 234,199 - AMB_RS01055 0.78 +0.2 234,253 - AMB_RS01055 0.81 +0.3 234,266 + AMB_RS01055 0.82 -0.4 234,309 + AMB_RS01055 0.85 +0.8 234,310 - AMB_RS01055 0.85 -0.6 235,013 + AMB_RS01060 0.73 +0.1 235,013 + AMB_RS01060 0.73 -0.3 235,013 + AMB_RS01060 0.73 +0.1 235,013 + AMB_RS01060 0.73 -2.8 235,013 + AMB_RS01060 0.73 +0.8 235,013 + AMB_RS01060 0.73 -1.8 235,013 + AMB_RS01060 0.73 +0.7 235,013 + AMB_RS01060 0.73 -0.9 235,013 + AMB_RS01060 0.73 -0.6 235,013 + AMB_RS01060 0.73 -1.6 235,014 - AMB_RS01060 0.73 -0.5 235,014 - AMB_RS01060 0.73 +0.2 235,014 - AMB_RS01060 0.73 -2.0 235,014 - AMB_RS01060 0.73 -4.4 235,014 - AMB_RS01060 0.73 -0.3 235,014 - AMB_RS01060 0.73 +0.7 235,118 + AMB_RS01060 0.88 -2.2 235,119 - AMB_RS01060 0.89 -0.6 235,175 + -2.4 235,175 + -0.6 235,176 - -0.6 235,176 - -0.1 235,213 + -0.6 235,213 + -0.9 235,213 + -1.3
Or see this region's nucleotide sequence