Experiment: 30_an_nonmag_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS00815 and AMB_RS00820 overlap by 1 nucleotides AMB_RS00820 and AMB_RS00825 are separated by 36 nucleotides AMB_RS00825 and AMB_RS00830 overlap by 1 nucleotides
AMB_RS00815: AMB_RS00815 - hypothetical protein, at 183,644 to 184,747
_RS00815
AMB_RS00820: AMB_RS00820 - GDP-L-fucose synthase, at 184,747 to 185,691
_RS00820
AMB_RS00825: AMB_RS00825 - NAD(+) kinase, at 185,728 to 186,495
_RS00825
AMB_RS00830: AMB_RS00830 - ammonia monooxygenase, at 186,495 to 187,541
_RS00830
Position (kb) 185
186
187 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 184.759 kb on + strand at 184.759 kb on + strand at 184.759 kb on + strand at 185.092 kb on + strand, within AMB_RS00820 at 185.093 kb on - strand, within AMB_RS00820 at 185.103 kb on - strand, within AMB_RS00820 at 185.278 kb on + strand, within AMB_RS00820 at 185.278 kb on + strand, within AMB_RS00820 at 185.278 kb on + strand, within AMB_RS00820 at 185.278 kb on + strand, within AMB_RS00820 at 185.279 kb on - strand, within AMB_RS00820 at 185.279 kb on - strand, within AMB_RS00820 at 185.279 kb on - strand, within AMB_RS00820 at 185.668 kb on + strand at 185.668 kb on + strand at 185.669 kb on - strand at 185.689 kb on + strand at 185.689 kb on + strand at 185.689 kb on + strand at 185.689 kb on + strand at 185.689 kb on + strand at 186.798 kb on + strand, within AMB_RS00830 at 186.798 kb on + strand, within AMB_RS00830 at 186.798 kb on + strand, within AMB_RS00830 at 186.798 kb on + strand, within AMB_RS00830 at 186.799 kb on - strand, within AMB_RS00830 at 186.803 kb on - strand, within AMB_RS00830 at 186.908 kb on + strand, within AMB_RS00830 at 186.908 kb on + strand, within AMB_RS00830 at 186.908 kb on + strand, within AMB_RS00830 at 186.908 kb on + strand, within AMB_RS00830 at 186.909 kb on - strand, within AMB_RS00830 at 187.313 kb on - strand, within AMB_RS00830
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_nonmag_3 remove 184,759 + +0.2 184,759 + -1.3 184,759 + +0.1 185,092 + AMB_RS00820 0.37 -3.4 185,093 - AMB_RS00820 0.37 -3.7 185,103 - AMB_RS00820 0.38 -2.9 185,278 + AMB_RS00820 0.56 -1.4 185,278 + AMB_RS00820 0.56 +0.1 185,278 + AMB_RS00820 0.56 -1.5 185,278 + AMB_RS00820 0.56 -4.8 185,279 - AMB_RS00820 0.56 -2.5 185,279 - AMB_RS00820 0.56 -1.8 185,279 - AMB_RS00820 0.56 -2.2 185,668 + +1.2 185,668 + -2.6 185,669 - -2.0 185,689 + -0.6 185,689 + +0.4 185,689 + -1.7 185,689 + -0.3 185,689 + +0.1 186,798 + AMB_RS00830 0.29 -1.8 186,798 + AMB_RS00830 0.29 -0.6 186,798 + AMB_RS00830 0.29 -2.2 186,798 + AMB_RS00830 0.29 -1.3 186,799 - AMB_RS00830 0.29 -0.3 186,803 - AMB_RS00830 0.29 +0.1 186,908 + AMB_RS00830 0.39 -4.6 186,908 + AMB_RS00830 0.39 -1.6 186,908 + AMB_RS00830 0.39 -0.3 186,908 + AMB_RS00830 0.39 +0.9 186,909 - AMB_RS00830 0.40 +0.3 187,313 - AMB_RS00830 0.78 +0.3
Or see this region's nucleotide sequence