Experiment: 30_an_nonmag_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS00680 and AMB_RS00685 overlap by 4 nucleotides AMB_RS00685 and AMB_RS00690 overlap by 104 nucleotides AMB_RS00690 and AMB_RS00695 are separated by 27 nucleotides AMB_RS00695 and AMB_RS00700 overlap by 23 nucleotides
AMB_RS00680: AMB_RS00680 - 3-dehydroquinate synthase, at 154,934 to 155,992
_RS00680
AMB_RS00685: AMB_RS00685 - phosphoglycerate dehydrogenase, at 155,989 to 156,945
_RS00685
AMB_RS00690: AMB_RS00690 - hypothetical protein, at 156,842 to 158,740
_RS00690
AMB_RS00695: AMB_RS00695 - gfo/Idh/MocA family oxidoreductase, at 158,768 to 159,712
_RS00695
AMB_RS00700: AMB_RS00700 - nucleoside-diphosphate sugar epimerase, at 159,690 to 160,751
_RS00700
Position (kb) 156
157
158
159 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 156.982 kb on + strand at 156.982 kb on + strand at 156.982 kb on + strand at 156.982 kb on + strand at 156.982 kb on + strand at 156.982 kb on + strand at 156.982 kb on + strand at 156.982 kb on + strand at 156.982 kb on + strand at 158.739 kb on - strand at 158.739 kb on - strand at 158.747 kb on + strand at 158.747 kb on + strand at 158.747 kb on + strand at 158.747 kb on + strand at 158.747 kb on + strand at 158.748 kb on - strand at 158.748 kb on - strand at 158.748 kb on - strand at 158.748 kb on - strand at 158.748 kb on - strand at 158.752 kb on - strand at 158.752 kb on - strand at 158.752 kb on - strand at 158.763 kb on + strand at 158.770 kb on - strand at 159.050 kb on + strand, within AMB_RS00695 at 159.334 kb on + strand, within AMB_RS00695 at 159.334 kb on + strand, within AMB_RS00695 at 159.529 kb on + strand, within AMB_RS00695 at 159.640 kb on + strand at 159.642 kb on + strand at 159.704 kb on + strand at 159.706 kb on + strand at 159.706 kb on + strand at 159.706 kb on + strand at 159.707 kb on - strand at 159.707 kb on - strand at 159.707 kb on - strand at 159.707 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_nonmag_3 remove 156,982 + -0.3 156,982 + -0.4 156,982 + -0.9 156,982 + -2.8 156,982 + +0.7 156,982 + -1.3 156,982 + -1.3 156,982 + +0.1 156,982 + +0.1 158,739 - -0.5 158,739 - -0.3 158,747 + -1.5 158,747 + +1.3 158,747 + -1.5 158,747 + +0.0 158,747 + -0.9 158,748 - -1.2 158,748 - -0.0 158,748 - -0.4 158,748 - -0.5 158,748 - -0.2 158,752 - -1.4 158,752 - +0.2 158,752 - +0.9 158,763 + -1.8 158,770 - -0.9 159,050 + AMB_RS00695 0.30 -2.0 159,334 + AMB_RS00695 0.60 -4.2 159,334 + AMB_RS00695 0.60 +0.7 159,529 + AMB_RS00695 0.81 -1.7 159,640 + -1.6 159,642 + -0.6 159,704 + -4.5 159,706 + -1.5 159,706 + -1.5 159,706 + -1.3 159,707 - +0.8 159,707 - -3.0 159,707 - -1.3 159,707 - -2.5
Or see this region's nucleotide sequence