Experiment: 30_an_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS21230 and AMB_RS21235 are separated by 200 nucleotides AMB_RS21235 and AMB_RS21240 overlap by 4 nucleotides AMB_RS21240 and AMB_RS21245 overlap by 4 nucleotides AMB_RS21245 and AMB_RS21250 are separated by 138 nucleotides
AMB_RS21230: AMB_RS21230 - xanthine dehydrogenase family protein molybdopterin-binding subunit, at 4,609,980 to 4,612,121
_RS21230
AMB_RS21235: AMB_RS21235 - heme transporter HemC, at 4,612,322 to 4,613,056
_RS21235
AMB_RS21240: AMB_RS21240 - heme exporter protein CcmD, at 4,613,053 to 4,613,262
_RS21240
AMB_RS21245: AMB_RS21245 - cytochrome c-type biogenesis protein CcmE, at 4,613,259 to 4,613,705
_RS21245
AMB_RS21250: AMB_RS21250 - heme lyase CcmF/NrfE family subunit, at 4,613,844 to 4,615,823
_RS21250
Position (kb) 4612
4613
4614 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4611.444 kb on + strand, within AMB_RS21230 at 4611.942 kb on + strand at 4612.119 kb on + strand at 4612.119 kb on + strand at 4612.119 kb on + strand at 4612.119 kb on + strand at 4612.119 kb on + strand at 4612.119 kb on + strand at 4612.119 kb on + strand at 4612.119 kb on + strand at 4612.119 kb on + strand at 4612.119 kb on + strand at 4612.119 kb on + strand at 4612.120 kb on - strand at 4612.120 kb on - strand at 4612.120 kb on - strand at 4612.120 kb on - strand at 4612.120 kb on - strand at 4613.828 kb on + strand at 4613.828 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_nonmag_2 remove 4,611,444 + AMB_RS21230 0.68 +0.7 4,611,942 + -2.4 4,612,119 + -0.2 4,612,119 + -0.3 4,612,119 + +0.3 4,612,119 + -2.2 4,612,119 + -2.8 4,612,119 + -0.0 4,612,119 + -1.1 4,612,119 + +1.7 4,612,119 + -2.6 4,612,119 + -1.1 4,612,119 + -1.3 4,612,120 - -0.1 4,612,120 - -1.9 4,612,120 - -0.6 4,612,120 - -0.3 4,612,120 - +0.0 4,613,828 + -0.4 4,613,828 + -2.6
Or see this region's nucleotide sequence