Experiment: 30_an_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS15270 and AMB_RS15275 are separated by 209 nucleotides AMB_RS15275 and AMB_RS15280 are separated by 175 nucleotides AMB_RS15280 and AMB_RS15285 overlap by 11 nucleotides
AMB_RS15265: AMB_RS15265 - Rrf2 family transcriptional regulator, at 3,272,507 to 3,272,980
_RS15265
AMB_RS15270: AMB_RS15270 - serine O-acetyltransferase, at 3,272,977 to 3,273,768
_RS15270
AMB_RS15275: AMB_RS15275 - alpha/beta hydrolase, at 3,273,978 to 3,274,673
_RS15275
AMB_RS15280: AMB_RS15280 - hypothetical protein, at 3,274,849 to 3,275,208
_RS15280
AMB_RS15285: AMB_RS15285 - anhydro-N-acetylmuramic acid kinase, at 3,275,198 to 3,276,265
_RS15285
Position (kb)
3273
3274
3275 Strain fitness (log2 ratio)
-2
-1
0
1 at 3273.035 kb on - strand at 3273.231 kb on - strand, within AMB_RS15270 at 3273.231 kb on - strand, within AMB_RS15270 at 3273.769 kb on - strand at 3273.914 kb on - strand at 3274.117 kb on - strand, within AMB_RS15275 at 3274.671 kb on + strand at 3274.672 kb on - strand at 3274.994 kb on + strand, within AMB_RS15280 at 3274.995 kb on - strand, within AMB_RS15280 at 3274.995 kb on - strand, within AMB_RS15280 at 3275.036 kb on + strand, within AMB_RS15280 at 3275.036 kb on + strand, within AMB_RS15280 at 3275.595 kb on + strand, within AMB_RS15285 at 3275.595 kb on + strand, within AMB_RS15285
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_nonmag_2 remove 3,273,035 - -0.9 3,273,231 - AMB_RS15270 0.32 -2.7 3,273,231 - AMB_RS15270 0.32 -1.7 3,273,769 - -2.2 3,273,914 - -2.5 3,274,117 - AMB_RS15275 0.20 -1.3 3,274,671 + -0.5 3,274,672 - -0.5 3,274,994 + AMB_RS15280 0.40 -1.5 3,274,995 - AMB_RS15280 0.41 -0.2 3,274,995 - AMB_RS15280 0.41 +0.5 3,275,036 + AMB_RS15280 0.52 +1.5 3,275,036 + AMB_RS15280 0.52 +1.0 3,275,595 + AMB_RS15285 0.37 -0.2 3,275,595 + AMB_RS15285 0.37 +1.4
Or see this region's nucleotide sequence