Experiment: 30_an_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS14485 and AMB_RS14490 are separated by 0 nucleotides AMB_RS14490 and AMB_RS14495 overlap by 4 nucleotides AMB_RS14495 and AMB_RS14500 overlap by 4 nucleotides AMB_RS14500 and AMB_RS14505 overlap by 4 nucleotides
AMB_RS14485: AMB_RS14485 - hypothetical protein, at 3,077,803 to 3,079,344
_RS14485
AMB_RS14490: AMB_RS14490 - iron-sulfur cluster assembly scaffold protein, at 3,079,345 to 3,079,746
_RS14490
AMB_RS14495: AMB_RS14495 - aminotransferase class V-fold PLP-dependent enzyme, at 3,079,743 to 3,080,945
_RS14495
AMB_RS14500: AMB_RS14500 - nucleotidyltransferase family protein, at 3,080,942 to 3,081,511
_RS14500
AMB_RS14505: AMB_RS14505 - carbon monoxide dehydrogenase, at 3,081,508 to 3,082,293
_RS14505
Position (kb)
3079
3080
3081 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3
4 at 3078.917 kb on - strand, within AMB_RS14485 at 3078.917 kb on - strand, within AMB_RS14485 at 3079.228 kb on + strand at 3079.228 kb on + strand at 3079.228 kb on + strand at 3079.228 kb on + strand at 3079.229 kb on - strand at 3079.229 kb on - strand at 3079.229 kb on - strand at 3079.259 kb on - strand at 3079.384 kb on + strand at 3079.385 kb on - strand at 3079.385 kb on - strand at 3079.810 kb on + strand at 3079.810 kb on + strand at 3080.290 kb on + strand, within AMB_RS14495 at 3080.290 kb on + strand, within AMB_RS14495 at 3080.291 kb on - strand, within AMB_RS14495 at 3080.314 kb on + strand, within AMB_RS14495 at 3080.314 kb on + strand, within AMB_RS14495 at 3080.315 kb on - strand, within AMB_RS14495 at 3080.315 kb on - strand, within AMB_RS14495 at 3080.761 kb on + strand, within AMB_RS14495 at 3080.890 kb on + strand at 3080.891 kb on - strand at 3081.557 kb on - strand at 3081.815 kb on + strand, within AMB_RS14505 at 3081.816 kb on - strand, within AMB_RS14505 at 3081.816 kb on - strand, within AMB_RS14505
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_nonmag_2 remove 3,078,917 - AMB_RS14485 0.72 +4.2 3,078,917 - AMB_RS14485 0.72 -2.6 3,079,228 + -0.9 3,079,228 + -1.8 3,079,228 + +0.7 3,079,228 + -0.5 3,079,229 - -1.4 3,079,229 - -1.4 3,079,229 - -1.5 3,079,259 - -0.3 3,079,384 + -1.5 3,079,385 - -5.3 3,079,385 - +1.5 3,079,810 + -4.1 3,079,810 + -0.5 3,080,290 + AMB_RS14495 0.45 -3.1 3,080,290 + AMB_RS14495 0.45 -3.8 3,080,291 - AMB_RS14495 0.46 -6.0 3,080,314 + AMB_RS14495 0.47 -2.1 3,080,314 + AMB_RS14495 0.47 -2.5 3,080,315 - AMB_RS14495 0.48 -4.0 3,080,315 - AMB_RS14495 0.48 -5.2 3,080,761 + AMB_RS14495 0.85 -0.9 3,080,890 + -4.9 3,080,891 - -2.9 3,081,557 - -2.2 3,081,815 + AMB_RS14505 0.39 -1.5 3,081,816 - AMB_RS14505 0.39 -3.0 3,081,816 - AMB_RS14505 0.39 -2.0
Or see this region's nucleotide sequence