Experiment: 30_an_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS13460 and AMB_RS13465 overlap by 4 nucleotides AMB_RS13465 and AMB_RS13470 overlap by 4 nucleotides AMB_RS13470 and AMB_RS13475 are separated by 58 nucleotides AMB_RS13475 and AMB_RS13480 are separated by 76 nucleotides
AMB_RS13460: AMB_RS13460 - transferase hexapeptide repeat family protein, at 2,866,727 to 2,867,344
_RS13460
AMB_RS13465: AMB_RS13465 - acyl-CoA thioesterase, at 2,867,341 to 2,867,784
_RS13465
AMB_RS13470: AMB_RS13470 - 3-hydroxybutyrate dehydrogenase, at 2,867,781 to 2,868,563
_RS13470
AMB_RS13475: AMB_RS13475 - DUF1192 domain-containing protein, at 2,868,622 to 2,868,804
_RS13475
AMB_RS13480: AMB_RS13480 - NAD(P)H quinone oxidoreductase, at 2,868,881 to 2,869,873
_RS13480
Position (kb)
2867
2868
2869 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2866.864 kb on - strand, within AMB_RS13460 at 2867.028 kb on + strand, within AMB_RS13460 at 2867.028 kb on + strand, within AMB_RS13460 at 2867.029 kb on - strand, within AMB_RS13460 at 2867.029 kb on - strand, within AMB_RS13460 at 2867.029 kb on - strand, within AMB_RS13460 at 2867.109 kb on + strand, within AMB_RS13460 at 2867.110 kb on - strand, within AMB_RS13460 at 2867.110 kb on - strand, within AMB_RS13460 at 2867.158 kb on + strand, within AMB_RS13460 at 2867.159 kb on - strand, within AMB_RS13460 at 2867.167 kb on + strand, within AMB_RS13460 at 2867.168 kb on - strand, within AMB_RS13460 at 2867.286 kb on + strand at 2867.286 kb on + strand at 2867.286 kb on + strand at 2867.286 kb on + strand at 2867.328 kb on + strand at 2867.328 kb on + strand at 2867.329 kb on - strand at 2867.511 kb on + strand, within AMB_RS13465 at 2867.511 kb on + strand, within AMB_RS13465 at 2867.511 kb on + strand, within AMB_RS13465 at 2867.512 kb on - strand, within AMB_RS13465 at 2867.550 kb on - strand, within AMB_RS13465 at 2867.655 kb on + strand, within AMB_RS13465 at 2867.655 kb on + strand, within AMB_RS13465 at 2867.655 kb on + strand, within AMB_RS13465 at 2867.655 kb on + strand, within AMB_RS13465 at 2867.656 kb on - strand, within AMB_RS13465 at 2867.656 kb on - strand, within AMB_RS13465 at 2867.656 kb on - strand, within AMB_RS13465 at 2867.656 kb on - strand, within AMB_RS13465 at 2867.675 kb on + strand, within AMB_RS13465 at 2867.676 kb on - strand, within AMB_RS13465 at 2867.676 kb on - strand, within AMB_RS13465 at 2867.775 kb on + strand at 2867.775 kb on + strand at 2867.775 kb on + strand at 2867.775 kb on + strand at 2867.775 kb on + strand at 2867.776 kb on - strand at 2868.100 kb on + strand, within AMB_RS13470 at 2868.100 kb on + strand, within AMB_RS13470 at 2868.101 kb on - strand, within AMB_RS13470 at 2868.388 kb on + strand, within AMB_RS13470 at 2868.388 kb on + strand, within AMB_RS13470 at 2868.388 kb on + strand, within AMB_RS13470 at 2868.388 kb on + strand, within AMB_RS13470 at 2868.389 kb on - strand, within AMB_RS13470 at 2868.389 kb on - strand, within AMB_RS13470 at 2868.389 kb on - strand, within AMB_RS13470 at 2868.389 kb on - strand, within AMB_RS13470 at 2868.589 kb on + strand at 2868.590 kb on - strand at 2868.719 kb on + strand, within AMB_RS13475 at 2868.719 kb on + strand, within AMB_RS13475 at 2868.720 kb on - strand, within AMB_RS13475 at 2868.720 kb on - strand, within AMB_RS13475 at 2868.839 kb on + strand at 2868.839 kb on + strand at 2868.840 kb on - strand at 2868.840 kb on - strand at 2868.864 kb on + strand at 2868.864 kb on + strand at 2868.864 kb on + strand at 2868.864 kb on + strand at 2868.864 kb on + strand at 2869.047 kb on - strand, within AMB_RS13480 at 2869.184 kb on + strand, within AMB_RS13480 at 2869.229 kb on + strand, within AMB_RS13480 at 2869.229 kb on + strand, within AMB_RS13480 at 2869.467 kb on - strand, within AMB_RS13480 at 2869.556 kb on - strand, within AMB_RS13480
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_nonmag_2 remove 2,866,864 - AMB_RS13460 0.22 -0.2 2,867,028 + AMB_RS13460 0.49 -0.6 2,867,028 + AMB_RS13460 0.49 -1.3 2,867,029 - AMB_RS13460 0.49 -1.2 2,867,029 - AMB_RS13460 0.49 -0.6 2,867,029 - AMB_RS13460 0.49 -0.0 2,867,109 + AMB_RS13460 0.62 -0.4 2,867,110 - AMB_RS13460 0.62 +0.2 2,867,110 - AMB_RS13460 0.62 -0.4 2,867,158 + AMB_RS13460 0.70 -0.3 2,867,159 - AMB_RS13460 0.70 +1.0 2,867,167 + AMB_RS13460 0.71 -0.9 2,867,168 - AMB_RS13460 0.71 -0.7 2,867,286 + -1.2 2,867,286 + -1.1 2,867,286 + -0.6 2,867,286 + -0.9 2,867,328 + -2.2 2,867,328 + +0.6 2,867,329 - -0.6 2,867,511 + AMB_RS13465 0.38 -0.7 2,867,511 + AMB_RS13465 0.38 +1.9 2,867,511 + AMB_RS13465 0.38 -2.4 2,867,512 - AMB_RS13465 0.39 -0.8 2,867,550 - AMB_RS13465 0.47 -1.4 2,867,655 + AMB_RS13465 0.71 +0.4 2,867,655 + AMB_RS13465 0.71 -4.0 2,867,655 + AMB_RS13465 0.71 -0.6 2,867,655 + AMB_RS13465 0.71 -0.3 2,867,656 - AMB_RS13465 0.71 -1.2 2,867,656 - AMB_RS13465 0.71 +0.2 2,867,656 - AMB_RS13465 0.71 -0.7 2,867,656 - AMB_RS13465 0.71 +0.1 2,867,675 + AMB_RS13465 0.75 -1.2 2,867,676 - AMB_RS13465 0.75 +1.0 2,867,676 - AMB_RS13465 0.75 -0.6 2,867,775 + -2.5 2,867,775 + -1.7 2,867,775 + -0.0 2,867,775 + -1.7 2,867,775 + -2.2 2,867,776 - -0.8 2,868,100 + AMB_RS13470 0.41 -1.6 2,868,100 + AMB_RS13470 0.41 -0.2 2,868,101 - AMB_RS13470 0.41 +0.6 2,868,388 + AMB_RS13470 0.78 +0.5 2,868,388 + AMB_RS13470 0.78 -0.6 2,868,388 + AMB_RS13470 0.78 -0.2 2,868,388 + AMB_RS13470 0.78 -0.3 2,868,389 - AMB_RS13470 0.78 -0.9 2,868,389 - AMB_RS13470 0.78 -3.8 2,868,389 - AMB_RS13470 0.78 -0.6 2,868,389 - AMB_RS13470 0.78 -1.1 2,868,589 + -1.7 2,868,590 - -0.7 2,868,719 + AMB_RS13475 0.53 -0.3 2,868,719 + AMB_RS13475 0.53 -1.9 2,868,720 - AMB_RS13475 0.54 -2.4 2,868,720 - AMB_RS13475 0.54 -1.0 2,868,839 + +0.3 2,868,839 + -0.9 2,868,840 - +1.4 2,868,840 - +0.4 2,868,864 + -1.7 2,868,864 + +0.0 2,868,864 + -1.9 2,868,864 + -0.9 2,868,864 + -1.6 2,869,047 - AMB_RS13480 0.17 -0.9 2,869,184 + AMB_RS13480 0.31 +0.1 2,869,229 + AMB_RS13480 0.35 +0.5 2,869,229 + AMB_RS13480 0.35 -0.9 2,869,467 - AMB_RS13480 0.59 -1.9 2,869,556 - AMB_RS13480 0.68 +0.2
Or see this region's nucleotide sequence