Experiment: 30_an_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS11130 and AMB_RS11135 overlap by 7 nucleotides AMB_RS11135 and AMB_RS11140 are separated by 111 nucleotides AMB_RS11140 and AMB_RS11145 overlap by 4 nucleotides AMB_RS11145 and AMB_RS11150 are separated by 44 nucleotides
AMB_RS11130: AMB_RS11130 - murein transglycosylase, at 2,395,605 to 2,396,444
_RS11130
AMB_RS11135: AMB_RS11135 - DedA family protein, at 2,396,438 to 2,397,046
_RS11135
AMB_RS11140: AMB_RS11140 - 5-(carboxyamino)imidazole ribonucleotide synthase, at 2,397,158 to 2,398,267
_RS11140
AMB_RS11145: AMB_RS11145 - 5-(carboxyamino)imidazole ribonucleotide mutase, at 2,398,264 to 2,398,785
_RS11145
AMB_RS11150: AMB_RS11150 - GGDEF domain-containing protein, at 2,398,830 to 2,399,675
_RS11150
Position (kb)
2397
2398
2399 Strain fitness (log2 ratio)
-2
-1
0
1 at 2396.355 kb on + strand, within AMB_RS11130 at 2396.355 kb on + strand, within AMB_RS11130 at 2396.379 kb on + strand at 2396.379 kb on + strand at 2397.064 kb on - strand at 2397.064 kb on - strand at 2397.064 kb on - strand at 2397.064 kb on - strand at 2397.064 kb on - strand at 2397.071 kb on - strand at 2397.071 kb on - strand at 2397.071 kb on - strand at 2397.097 kb on - strand at 2397.131 kb on - strand at 2397.151 kb on - strand at 2397.151 kb on - strand at 2398.806 kb on - strand at 2398.896 kb on + strand at 2398.896 kb on + strand at 2398.897 kb on - strand at 2399.212 kb on - strand, within AMB_RS11150
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_nonmag_2 remove 2,396,355 + AMB_RS11130 0.89 -1.5 2,396,355 + AMB_RS11130 0.89 +0.4 2,396,379 + -0.1 2,396,379 + +0.4 2,397,064 - -1.4 2,397,064 - -0.2 2,397,064 - -0.8 2,397,064 - -1.4 2,397,064 - -0.7 2,397,071 - -1.7 2,397,071 - -2.1 2,397,071 - -0.2 2,397,097 - -1.6 2,397,131 - -1.0 2,397,151 - +0.4 2,397,151 - -2.0 2,398,806 - -1.6 2,398,896 + +1.2 2,398,896 + +0.2 2,398,897 - -1.4 2,399,212 - AMB_RS11150 0.45 -1.0
Or see this region's nucleotide sequence