Experiment: 30_an_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS08275 and AMB_RS08280 overlap by 1 nucleotides AMB_RS08280 and AMB_RS08285 are separated by 0 nucleotides AMB_RS08285 and AMB_RS08290 are separated by 3 nucleotides AMB_RS08290 and AMB_RS08295 overlap by 8 nucleotides
AMB_RS08275: AMB_RS08275 - hydrogenase maturation protein, at 1,774,823 to 1,776,475
_RS08275
AMB_RS08280: AMB_RS08280 - hydrogenase expression/formation protein HypE, at 1,776,475 to 1,777,524
_RS08280
AMB_RS08285: AMB_RS08285 - DUF2442 domain-containing protein, at 1,777,525 to 1,777,788
_RS08285
AMB_RS08290: AMB_RS08290 - DUF4160 domain-containing protein, at 1,777,792 to 1,778,052
_RS08290
AMB_RS08295: AMB_RS08295 - hydrogenase formation protein HypD, at 1,778,045 to 1,779,184
_RS08295
Position (kb) 1776
1777
1778 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1775.619 kb on + strand, within AMB_RS08275 at 1775.620 kb on - strand, within AMB_RS08275 at 1775.620 kb on - strand, within AMB_RS08275 at 1775.878 kb on + strand, within AMB_RS08275 at 1775.878 kb on + strand, within AMB_RS08275 at 1776.170 kb on + strand, within AMB_RS08275 at 1776.170 kb on + strand, within AMB_RS08275 at 1776.171 kb on - strand, within AMB_RS08275 at 1776.171 kb on - strand, within AMB_RS08275 at 1776.462 kb on - strand at 1776.462 kb on - strand at 1776.660 kb on + strand, within AMB_RS08280 at 1776.661 kb on - strand, within AMB_RS08280 at 1776.661 kb on - strand, within AMB_RS08280 at 1776.689 kb on + strand, within AMB_RS08280 at 1776.689 kb on + strand, within AMB_RS08280 at 1776.882 kb on + strand, within AMB_RS08280 at 1776.882 kb on + strand, within AMB_RS08280 at 1776.882 kb on + strand, within AMB_RS08280 at 1776.882 kb on + strand, within AMB_RS08280 at 1776.882 kb on + strand, within AMB_RS08280 at 1776.883 kb on - strand, within AMB_RS08280 at 1776.883 kb on - strand, within AMB_RS08280 at 1776.883 kb on - strand, within AMB_RS08280 at 1776.883 kb on - strand, within AMB_RS08280 at 1776.883 kb on - strand, within AMB_RS08280 at 1776.883 kb on - strand, within AMB_RS08280 at 1776.883 kb on - strand, within AMB_RS08280 at 1776.883 kb on - strand, within AMB_RS08280 at 1776.883 kb on - strand, within AMB_RS08280 at 1777.316 kb on + strand, within AMB_RS08280 at 1777.316 kb on + strand, within AMB_RS08280 at 1777.316 kb on + strand, within AMB_RS08280 at 1777.317 kb on - strand, within AMB_RS08280 at 1777.317 kb on - strand, within AMB_RS08280 at 1777.794 kb on - strand at 1777.794 kb on - strand at 1777.885 kb on + strand, within AMB_RS08290 at 1777.885 kb on + strand, within AMB_RS08290 at 1777.885 kb on + strand, within AMB_RS08290 at 1777.885 kb on + strand, within AMB_RS08290 at 1777.885 kb on + strand, within AMB_RS08290 at 1777.886 kb on - strand, within AMB_RS08290 at 1777.886 kb on - strand, within AMB_RS08290 at 1777.971 kb on - strand, within AMB_RS08290 at 1778.007 kb on - strand, within AMB_RS08290 at 1778.007 kb on - strand, within AMB_RS08290 at 1778.009 kb on + strand, within AMB_RS08290 at 1778.009 kb on + strand, within AMB_RS08290 at 1778.009 kb on + strand, within AMB_RS08290 at 1778.009 kb on + strand, within AMB_RS08290 at 1778.010 kb on - strand, within AMB_RS08290 at 1778.010 kb on - strand, within AMB_RS08290 at 1778.010 kb on - strand, within AMB_RS08290 at 1778.010 kb on - strand, within AMB_RS08290 at 1778.010 kb on - strand, within AMB_RS08290 at 1778.082 kb on + strand at 1778.082 kb on + strand at 1778.083 kb on - strand at 1778.283 kb on + strand, within AMB_RS08295 at 1778.284 kb on - strand, within AMB_RS08295 at 1778.311 kb on - strand, within AMB_RS08295
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_nonmag_2 remove 1,775,619 + AMB_RS08275 0.48 +1.8 1,775,620 - AMB_RS08275 0.48 -0.2 1,775,620 - AMB_RS08275 0.48 -0.5 1,775,878 + AMB_RS08275 0.64 -0.6 1,775,878 + AMB_RS08275 0.64 -0.7 1,776,170 + AMB_RS08275 0.81 -0.2 1,776,170 + AMB_RS08275 0.81 -1.5 1,776,171 - AMB_RS08275 0.82 -0.6 1,776,171 - AMB_RS08275 0.82 -1.4 1,776,462 - -0.5 1,776,462 - -0.9 1,776,660 + AMB_RS08280 0.18 -0.6 1,776,661 - AMB_RS08280 0.18 -2.1 1,776,661 - AMB_RS08280 0.18 -0.9 1,776,689 + AMB_RS08280 0.20 +0.8 1,776,689 + AMB_RS08280 0.20 -0.9 1,776,882 + AMB_RS08280 0.39 +0.4 1,776,882 + AMB_RS08280 0.39 +0.4 1,776,882 + AMB_RS08280 0.39 -0.7 1,776,882 + AMB_RS08280 0.39 -0.0 1,776,882 + AMB_RS08280 0.39 +0.5 1,776,883 - AMB_RS08280 0.39 -1.8 1,776,883 - AMB_RS08280 0.39 -1.6 1,776,883 - AMB_RS08280 0.39 -1.4 1,776,883 - AMB_RS08280 0.39 -1.8 1,776,883 - AMB_RS08280 0.39 -1.9 1,776,883 - AMB_RS08280 0.39 -1.0 1,776,883 - AMB_RS08280 0.39 -0.4 1,776,883 - AMB_RS08280 0.39 -1.3 1,776,883 - AMB_RS08280 0.39 -0.9 1,777,316 + AMB_RS08280 0.80 -0.4 1,777,316 + AMB_RS08280 0.80 -0.1 1,777,316 + AMB_RS08280 0.80 -1.0 1,777,317 - AMB_RS08280 0.80 -0.6 1,777,317 - AMB_RS08280 0.80 -0.3 1,777,794 - -0.2 1,777,794 - -1.9 1,777,885 + AMB_RS08290 0.36 +0.4 1,777,885 + AMB_RS08290 0.36 +0.4 1,777,885 + AMB_RS08290 0.36 -0.2 1,777,885 + AMB_RS08290 0.36 -0.6 1,777,885 + AMB_RS08290 0.36 -2.4 1,777,886 - AMB_RS08290 0.36 -1.7 1,777,886 - AMB_RS08290 0.36 -3.6 1,777,971 - AMB_RS08290 0.69 -1.1 1,778,007 - AMB_RS08290 0.82 -1.6 1,778,007 - AMB_RS08290 0.82 +0.4 1,778,009 + AMB_RS08290 0.83 -2.5 1,778,009 + AMB_RS08290 0.83 -0.9 1,778,009 + AMB_RS08290 0.83 -0.9 1,778,009 + AMB_RS08290 0.83 -2.3 1,778,010 - AMB_RS08290 0.84 -2.3 1,778,010 - AMB_RS08290 0.84 -0.9 1,778,010 - AMB_RS08290 0.84 -0.3 1,778,010 - AMB_RS08290 0.84 -0.1 1,778,010 - AMB_RS08290 0.84 -0.5 1,778,082 + +0.3 1,778,082 + +1.4 1,778,083 - -0.1 1,778,283 + AMB_RS08295 0.21 -0.5 1,778,284 - AMB_RS08295 0.21 -0.6 1,778,311 - AMB_RS08295 0.23 -1.2
Or see this region's nucleotide sequence