Experiment: 30_an_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS24275 and AMB_RS07960 are separated by 236 nucleotides AMB_RS07960 and AMB_RS07965 are separated by 32 nucleotides AMB_RS07965 and AMB_RS07970 are separated by 5 nucleotides AMB_RS07970 and AMB_RS07975 overlap by 4 nucleotides AMB_RS07975 and AMB_RS07980 are separated by 80 nucleotides AMB_RS07980 and AMB_RS07985 are separated by 20 nucleotides
AMB_RS24275: AMB_RS24275 - methyl-accepting chemotaxis protein, at 1,707,396 to 1,709,417
_RS24275
AMB_RS07960: AMB_RS07960 - ferredoxin, at 1,709,654 to 1,710,037
_RS07960
AMB_RS07965: AMB_RS07965 - SIR2 family protein, at 1,710,070 to 1,710,942
_RS07965
AMB_RS07970: AMB_RS07970 - putative nitrogen fixation protein NifT, at 1,710,948 to 1,711,163
_RS07970
AMB_RS07975: AMB_RS07975 - nitrogen fixation protein NifZ, at 1,711,160 to 1,711,441
_RS07975
AMB_RS07980: AMB_RS07980 - 4Fe-4S ferredoxin, at 1,711,522 to 1,711,710
_RS07980
AMB_RS07985: AMB_RS07985 - nitrogenase cofactor biosynthesis protein NifB, at 1,711,731 to 1,713,227
_RS07985
Position (kb)
1710
1711 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1709.194 kb on + strand, within AMB_RS24275 at 1709.195 kb on - strand, within AMB_RS24275 at 1709.195 kb on - strand, within AMB_RS24275 at 1709.195 kb on - strand, within AMB_RS24275 at 1709.388 kb on - strand at 1709.388 kb on - strand at 1709.398 kb on - strand at 1709.464 kb on + strand at 1709.518 kb on - strand at 1709.523 kb on + strand at 1709.656 kb on - strand at 1709.727 kb on + strand, within AMB_RS07960 at 1709.728 kb on - strand, within AMB_RS07960 at 1709.985 kb on + strand, within AMB_RS07960 at 1709.985 kb on + strand, within AMB_RS07960 at 1710.194 kb on + strand, within AMB_RS07965 at 1710.254 kb on + strand, within AMB_RS07965 at 1710.254 kb on + strand, within AMB_RS07965 at 1710.254 kb on + strand, within AMB_RS07965 at 1710.254 kb on + strand, within AMB_RS07965 at 1710.254 kb on + strand, within AMB_RS07965 at 1710.254 kb on + strand, within AMB_RS07965 at 1710.255 kb on - strand, within AMB_RS07965 at 1710.255 kb on - strand, within AMB_RS07965 at 1710.255 kb on - strand, within AMB_RS07965 at 1710.255 kb on - strand, within AMB_RS07965 at 1710.255 kb on - strand, within AMB_RS07965 at 1710.356 kb on + strand, within AMB_RS07965 at 1710.356 kb on + strand, within AMB_RS07965 at 1710.356 kb on + strand, within AMB_RS07965 at 1710.356 kb on + strand, within AMB_RS07965 at 1710.356 kb on + strand, within AMB_RS07965 at 1710.357 kb on - strand, within AMB_RS07965 at 1710.413 kb on + strand, within AMB_RS07965 at 1710.413 kb on + strand, within AMB_RS07965 at 1710.413 kb on + strand, within AMB_RS07965 at 1710.413 kb on + strand, within AMB_RS07965 at 1710.413 kb on + strand, within AMB_RS07965 at 1710.413 kb on + strand, within AMB_RS07965 at 1710.413 kb on + strand, within AMB_RS07965 at 1710.414 kb on - strand, within AMB_RS07965 at 1710.414 kb on - strand, within AMB_RS07965 at 1710.719 kb on + strand, within AMB_RS07965 at 1710.742 kb on - strand, within AMB_RS07965 at 1711.120 kb on + strand, within AMB_RS07970 at 1711.120 kb on + strand, within AMB_RS07970 at 1711.120 kb on + strand, within AMB_RS07970 at 1711.120 kb on + strand, within AMB_RS07970 at 1711.120 kb on + strand, within AMB_RS07970 at 1711.120 kb on + strand, within AMB_RS07970 at 1711.121 kb on - strand, within AMB_RS07970 at 1711.121 kb on - strand, within AMB_RS07970 at 1711.251 kb on + strand, within AMB_RS07975 at 1711.251 kb on + strand, within AMB_RS07975 at 1711.252 kb on - strand, within AMB_RS07975 at 1711.257 kb on + strand, within AMB_RS07975 at 1711.257 kb on + strand, within AMB_RS07975 at 1711.257 kb on + strand, within AMB_RS07975 at 1711.257 kb on + strand, within AMB_RS07975 at 1711.257 kb on + strand, within AMB_RS07975 at 1711.257 kb on + strand, within AMB_RS07975 at 1711.257 kb on + strand, within AMB_RS07975 at 1711.258 kb on - strand, within AMB_RS07975 at 1711.258 kb on - strand, within AMB_RS07975 at 1711.258 kb on - strand, within AMB_RS07975 at 1711.258 kb on - strand, within AMB_RS07975 at 1711.272 kb on + strand, within AMB_RS07975 at 1711.272 kb on + strand, within AMB_RS07975 at 1711.273 kb on - strand, within AMB_RS07975 at 1711.273 kb on - strand, within AMB_RS07975 at 1711.273 kb on - strand, within AMB_RS07975 at 1711.293 kb on + strand, within AMB_RS07975 at 1711.293 kb on + strand, within AMB_RS07975 at 1711.294 kb on - strand, within AMB_RS07975 at 1711.399 kb on - strand, within AMB_RS07975 at 1711.399 kb on - strand, within AMB_RS07975 at 1711.399 kb on - strand, within AMB_RS07975 at 1711.616 kb on + strand, within AMB_RS07980 at 1711.616 kb on + strand, within AMB_RS07980 at 1711.616 kb on + strand, within AMB_RS07980 at 1711.617 kb on - strand, within AMB_RS07980 at 1711.617 kb on - strand, within AMB_RS07980 at 1711.777 kb on + strand at 1711.804 kb on + strand at 1711.804 kb on + strand at 1711.805 kb on - strand at 1711.917 kb on + strand, within AMB_RS07985 at 1711.917 kb on + strand, within AMB_RS07985 at 1711.918 kb on - strand, within AMB_RS07985
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_nonmag_2 remove 1,709,194 + AMB_RS24275 0.89 +0.5 1,709,195 - AMB_RS24275 0.89 -1.0 1,709,195 - AMB_RS24275 0.89 +1.4 1,709,195 - AMB_RS24275 0.89 -2.2 1,709,388 - +0.4 1,709,388 - +1.4 1,709,398 - -0.3 1,709,464 + -0.9 1,709,518 - -1.9 1,709,523 + -0.9 1,709,656 - -2.3 1,709,727 + AMB_RS07960 0.19 -1.4 1,709,728 - AMB_RS07960 0.19 +0.4 1,709,985 + AMB_RS07960 0.86 +2.3 1,709,985 + AMB_RS07960 0.86 -0.2 1,710,194 + AMB_RS07965 0.14 -1.9 1,710,254 + AMB_RS07965 0.21 +1.4 1,710,254 + AMB_RS07965 0.21 -2.0 1,710,254 + AMB_RS07965 0.21 -1.9 1,710,254 + AMB_RS07965 0.21 -1.4 1,710,254 + AMB_RS07965 0.21 -0.9 1,710,254 + AMB_RS07965 0.21 -2.6 1,710,255 - AMB_RS07965 0.21 +0.3 1,710,255 - AMB_RS07965 0.21 -2.5 1,710,255 - AMB_RS07965 0.21 -2.4 1,710,255 - AMB_RS07965 0.21 -2.4 1,710,255 - AMB_RS07965 0.21 -0.1 1,710,356 + AMB_RS07965 0.33 +0.0 1,710,356 + AMB_RS07965 0.33 +0.5 1,710,356 + AMB_RS07965 0.33 +0.0 1,710,356 + AMB_RS07965 0.33 -1.7 1,710,356 + AMB_RS07965 0.33 -1.4 1,710,357 - AMB_RS07965 0.33 -0.2 1,710,413 + AMB_RS07965 0.39 -1.6 1,710,413 + AMB_RS07965 0.39 -0.4 1,710,413 + AMB_RS07965 0.39 -0.2 1,710,413 + AMB_RS07965 0.39 -2.2 1,710,413 + AMB_RS07965 0.39 -1.4 1,710,413 + AMB_RS07965 0.39 -0.2 1,710,413 + AMB_RS07965 0.39 -0.0 1,710,414 - AMB_RS07965 0.39 -1.1 1,710,414 - AMB_RS07965 0.39 -1.3 1,710,719 + AMB_RS07965 0.74 -1.9 1,710,742 - AMB_RS07965 0.77 +0.1 1,711,120 + AMB_RS07970 0.80 +0.5 1,711,120 + AMB_RS07970 0.80 -1.1 1,711,120 + AMB_RS07970 0.80 -0.9 1,711,120 + AMB_RS07970 0.80 -3.5 1,711,120 + AMB_RS07970 0.80 -0.4 1,711,120 + AMB_RS07970 0.80 +0.4 1,711,121 - AMB_RS07970 0.80 -0.4 1,711,121 - AMB_RS07970 0.80 -0.1 1,711,251 + AMB_RS07975 0.32 +0.3 1,711,251 + AMB_RS07975 0.32 -0.2 1,711,252 - AMB_RS07975 0.33 -0.3 1,711,257 + AMB_RS07975 0.34 -1.3 1,711,257 + AMB_RS07975 0.34 +0.9 1,711,257 + AMB_RS07975 0.34 -0.8 1,711,257 + AMB_RS07975 0.34 -0.9 1,711,257 + AMB_RS07975 0.34 +0.4 1,711,257 + AMB_RS07975 0.34 -0.6 1,711,257 + AMB_RS07975 0.34 -0.2 1,711,258 - AMB_RS07975 0.35 -1.2 1,711,258 - AMB_RS07975 0.35 -0.6 1,711,258 - AMB_RS07975 0.35 -2.5 1,711,258 - AMB_RS07975 0.35 -0.5 1,711,272 + AMB_RS07975 0.40 -1.9 1,711,272 + AMB_RS07975 0.40 -2.8 1,711,273 - AMB_RS07975 0.40 -1.9 1,711,273 - AMB_RS07975 0.40 -0.7 1,711,273 - AMB_RS07975 0.40 -2.8 1,711,293 + AMB_RS07975 0.47 -3.4 1,711,293 + AMB_RS07975 0.47 -2.0 1,711,294 - AMB_RS07975 0.48 -1.2 1,711,399 - AMB_RS07975 0.85 +0.2 1,711,399 - AMB_RS07975 0.85 -0.4 1,711,399 - AMB_RS07975 0.85 -1.2 1,711,616 + AMB_RS07980 0.50 +0.4 1,711,616 + AMB_RS07980 0.50 -1.6 1,711,616 + AMB_RS07980 0.50 +0.0 1,711,617 - AMB_RS07980 0.50 -2.2 1,711,617 - AMB_RS07980 0.50 +0.0 1,711,777 + -2.2 1,711,804 + -1.1 1,711,804 + -0.6 1,711,805 - -1.2 1,711,917 + AMB_RS07985 0.12 -1.8 1,711,917 + AMB_RS07985 0.12 -1.9 1,711,918 - AMB_RS07985 0.12 -0.5
Or see this region's nucleotide sequence