Experiment: 30_an_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS06920 and AMB_RS06925 are separated by 210 nucleotides AMB_RS06925 and AMB_RS06930 overlap by 54 nucleotides AMB_RS06930 and AMB_RS06935 are separated by 237 nucleotides AMB_RS06935 and AMB_RS06940 overlap by 1 nucleotides
AMB_RS06920: AMB_RS06920 - squalene--hopene cyclase, at 1,482,658 to 1,484,612
_RS06920
AMB_RS06925: AMB_RS06925 - nucleoside phosphorylase, at 1,484,823 to 1,485,473
_RS06925
AMB_RS06930: AMB_RS06930 - hopanoid biosynthesis associated radical SAM protein HpnH, at 1,485,420 to 1,486,571
_RS06930
AMB_RS06935: AMB_RS06935 - phosphate regulon transcriptional regulatory protein PhoB, at 1,486,809 to 1,487,531
_RS06935
AMB_RS06940: AMB_RS06940 - phosphate transport system regulatory protein PhoU, at 1,487,531 to 1,488,226
_RS06940
Position (kb)
1485
1486
1487 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1484.807 kb on + strand at 1485.506 kb on - strand at 1486.675 kb on + strand at 1486.693 kb on + strand at 1486.939 kb on + strand, within AMB_RS06935 at 1486.939 kb on + strand, within AMB_RS06935 at 1486.939 kb on + strand, within AMB_RS06935 at 1486.939 kb on + strand, within AMB_RS06935 at 1486.939 kb on + strand, within AMB_RS06935 at 1486.939 kb on + strand, within AMB_RS06935 at 1486.939 kb on + strand, within AMB_RS06935 at 1486.940 kb on - strand, within AMB_RS06935 at 1486.940 kb on - strand, within AMB_RS06935 at 1486.940 kb on - strand, within AMB_RS06935 at 1486.940 kb on - strand, within AMB_RS06935 at 1487.201 kb on - strand, within AMB_RS06935 at 1487.446 kb on + strand, within AMB_RS06935 at 1487.447 kb on - strand, within AMB_RS06935 at 1487.447 kb on - strand, within AMB_RS06935 at 1487.532 kb on + strand at 1487.533 kb on - strand at 1487.533 kb on - strand at 1487.533 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_nonmag_2 remove 1,484,807 + -1.2 1,485,506 - -2.0 1,486,675 + +0.4 1,486,693 + +1.7 1,486,939 + AMB_RS06935 0.18 -1.6 1,486,939 + AMB_RS06935 0.18 -0.9 1,486,939 + AMB_RS06935 0.18 -0.3 1,486,939 + AMB_RS06935 0.18 -0.0 1,486,939 + AMB_RS06935 0.18 -0.5 1,486,939 + AMB_RS06935 0.18 -0.2 1,486,939 + AMB_RS06935 0.18 +0.0 1,486,940 - AMB_RS06935 0.18 -0.3 1,486,940 - AMB_RS06935 0.18 +1.4 1,486,940 - AMB_RS06935 0.18 +1.2 1,486,940 - AMB_RS06935 0.18 -0.2 1,487,201 - AMB_RS06935 0.54 -3.2 1,487,446 + AMB_RS06935 0.88 -1.7 1,487,447 - AMB_RS06935 0.88 -0.2 1,487,447 - AMB_RS06935 0.88 -0.8 1,487,532 + -1.6 1,487,533 - -0.3 1,487,533 - -1.4 1,487,533 - +0.2
Or see this region's nucleotide sequence