Experiment: 30_an_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01850 and AMB_RS01855 are separated by 258 nucleotides AMB_RS01855 and AMB_RS01860 are separated by 90 nucleotides AMB_RS01860 and AMB_RS01865 are separated by 3 nucleotides AMB_RS01865 and AMB_RS01870 overlap by 25 nucleotides AMB_RS01870 and AMB_RS01875 are separated by 100 nucleotides AMB_RS01875 and AMB_RS01880 are separated by 42 nucleotides AMB_RS01880 and AMB_RS01885 are separated by 70 nucleotides
AMB_RS01850: AMB_RS01850 - hypothetical protein, at 399,006 to 399,221
_RS01850
AMB_RS01855: AMB_RS01855 - hypothetical protein, at 399,480 to 399,689
_RS01855
AMB_RS01860: AMB_RS01860 - HU family DNA-binding protein, at 399,780 to 400,034
_RS01860
AMB_RS01865: AMB_RS01865 - hypothetical protein, at 400,038 to 400,226
_RS01865
AMB_RS01870: AMB_RS01870 - DUF3489 domain-containing protein, at 400,202 to 400,792
_RS01870
AMB_RS01875: AMB_RS01875 - hypothetical protein, at 400,893 to 401,072
_RS01875
AMB_RS01880: AMB_RS01880 - hypothetical protein, at 401,115 to 401,507
_RS01880
AMB_RS01885: AMB_RS01885 - hypothetical protein, at 401,578 to 402,129
_RS01885
Position (kb) 400
401 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 399.426 kb on + strand at 399.427 kb on - strand at 399.469 kb on - strand at 399.537 kb on + strand, within AMB_RS01855 at 399.568 kb on + strand, within AMB_RS01855 at 399.568 kb on + strand, within AMB_RS01855 at 399.654 kb on + strand, within AMB_RS01855 at 399.654 kb on + strand, within AMB_RS01855 at 399.655 kb on - strand, within AMB_RS01855 at 399.655 kb on - strand, within AMB_RS01855 at 399.685 kb on + strand at 399.685 kb on + strand at 399.685 kb on + strand at 399.686 kb on - strand at 399.686 kb on - strand at 399.696 kb on + strand at 399.729 kb on + strand at 399.729 kb on + strand at 399.729 kb on + strand at 399.770 kb on + strand at 399.770 kb on + strand at 399.771 kb on - strand at 400.032 kb on + strand at 400.032 kb on + strand at 400.033 kb on - strand at 400.092 kb on + strand, within AMB_RS01865 at 400.139 kb on - strand, within AMB_RS01865 at 400.324 kb on + strand, within AMB_RS01870 at 400.324 kb on + strand, within AMB_RS01870 at 400.324 kb on + strand, within AMB_RS01870 at 400.430 kb on - strand, within AMB_RS01870 at 400.430 kb on - strand, within AMB_RS01870 at 400.554 kb on + strand, within AMB_RS01870 at 400.554 kb on + strand, within AMB_RS01870 at 400.554 kb on + strand, within AMB_RS01870 at 400.554 kb on + strand, within AMB_RS01870 at 400.554 kb on + strand, within AMB_RS01870 at 400.555 kb on - strand, within AMB_RS01870 at 400.555 kb on - strand, within AMB_RS01870 at 400.555 kb on - strand, within AMB_RS01870 at 400.555 kb on - strand, within AMB_RS01870 at 400.555 kb on - strand, within AMB_RS01870 at 400.837 kb on + strand at 400.838 kb on - strand at 400.931 kb on - strand, within AMB_RS01875 at 400.931 kb on - strand, within AMB_RS01875 at 400.931 kb on - strand, within AMB_RS01875 at 400.931 kb on - strand, within AMB_RS01875 at 400.980 kb on + strand, within AMB_RS01875 at 400.980 kb on + strand, within AMB_RS01875 at 400.981 kb on - strand, within AMB_RS01875 at 400.981 kb on - strand, within AMB_RS01875 at 401.076 kb on + strand at 401.077 kb on - strand at 401.077 kb on - strand at 401.077 kb on - strand at 401.167 kb on + strand, within AMB_RS01880 at 401.168 kb on - strand, within AMB_RS01880 at 401.168 kb on - strand, within AMB_RS01880 at 401.543 kb on - strand at 401.566 kb on - strand at 401.599 kb on + strand at 401.599 kb on + strand at 401.599 kb on + strand at 401.599 kb on + strand at 401.600 kb on - strand at 401.600 kb on - strand at 401.600 kb on - strand at 401.600 kb on - strand at 401.743 kb on - strand, within AMB_RS01885 at 401.790 kb on + strand, within AMB_RS01885 at 401.790 kb on + strand, within AMB_RS01885 at 401.790 kb on + strand, within AMB_RS01885 at 401.791 kb on - strand, within AMB_RS01885 at 401.791 kb on - strand, within AMB_RS01885 at 401.791 kb on - strand, within AMB_RS01885 at 401.791 kb on - strand, within AMB_RS01885 at 401.791 kb on - strand, within AMB_RS01885
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_nonmag_2 remove 399,426 + -1.1 399,427 - -0.6 399,469 - -0.3 399,537 + AMB_RS01855 0.27 -0.6 399,568 + AMB_RS01855 0.42 -0.3 399,568 + AMB_RS01855 0.42 -1.3 399,654 + AMB_RS01855 0.83 -1.6 399,654 + AMB_RS01855 0.83 -0.6 399,655 - AMB_RS01855 0.83 +0.1 399,655 - AMB_RS01855 0.83 +0.7 399,685 + -0.9 399,685 + +0.7 399,685 + -1.1 399,686 - +0.0 399,686 - -0.4 399,696 + +4.4 399,729 + -0.6 399,729 + -0.9 399,729 + +0.7 399,770 + +0.7 399,770 + -1.8 399,771 - +0.5 400,032 + -0.6 400,032 + -0.6 400,033 - -0.4 400,092 + AMB_RS01865 0.29 -0.8 400,139 - AMB_RS01865 0.53 -0.5 400,324 + AMB_RS01870 0.21 -0.4 400,324 + AMB_RS01870 0.21 -0.1 400,324 + AMB_RS01870 0.21 -0.7 400,430 - AMB_RS01870 0.39 -0.3 400,430 - AMB_RS01870 0.39 -0.9 400,554 + AMB_RS01870 0.60 -2.0 400,554 + AMB_RS01870 0.60 -0.3 400,554 + AMB_RS01870 0.60 -0.4 400,554 + AMB_RS01870 0.60 -0.6 400,554 + AMB_RS01870 0.60 -0.6 400,555 - AMB_RS01870 0.60 -1.3 400,555 - AMB_RS01870 0.60 +1.7 400,555 - AMB_RS01870 0.60 -0.5 400,555 - AMB_RS01870 0.60 -1.1 400,555 - AMB_RS01870 0.60 -0.3 400,837 + +0.1 400,838 - +0.9 400,931 - AMB_RS01875 0.21 +0.7 400,931 - AMB_RS01875 0.21 -0.6 400,931 - AMB_RS01875 0.21 -0.9 400,931 - AMB_RS01875 0.21 -0.6 400,980 + AMB_RS01875 0.48 -0.8 400,980 + AMB_RS01875 0.48 -0.7 400,981 - AMB_RS01875 0.49 -0.6 400,981 - AMB_RS01875 0.49 -2.6 401,076 + -0.2 401,077 - +0.1 401,077 - -2.4 401,077 - +0.1 401,167 + AMB_RS01880 0.13 -1.7 401,168 - AMB_RS01880 0.13 -1.8 401,168 - AMB_RS01880 0.13 +0.1 401,543 - -0.6 401,566 - -0.2 401,599 + -0.7 401,599 + +0.2 401,599 + -0.8 401,599 + -0.7 401,600 - -0.5 401,600 - -0.6 401,600 - -2.9 401,600 - -0.3 401,743 - AMB_RS01885 0.30 +0.1 401,790 + AMB_RS01885 0.38 +0.2 401,790 + AMB_RS01885 0.38 -0.5 401,790 + AMB_RS01885 0.38 -0.2 401,791 - AMB_RS01885 0.39 +1.1 401,791 - AMB_RS01885 0.39 -0.8 401,791 - AMB_RS01885 0.39 -0.5 401,791 - AMB_RS01885 0.39 -0.0 401,791 - AMB_RS01885 0.39 -0.9
Or see this region's nucleotide sequence