Experiment: 30_an_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS00920 and AMB_RS00925 overlap by 4 nucleotides AMB_RS00925 and AMB_RS00930 overlap by 4 nucleotides AMB_RS00930 and AMB_RS00935 are separated by 154 nucleotides
AMB_RS00920: AMB_RS00920 - secretion protein HlyD, at 208,887 to 209,828
_RS00920
AMB_RS00925: AMB_RS00925 - ABC transporter ATP-binding protein, at 209,825 to 210,751
_RS00925
AMB_RS00930: AMB_RS00930 - ABC transporter permease, at 210,748 to 211,890
_RS00930
AMB_RS00935: AMB_RS00935 - methyl-accepting chemotaxis protein, at 212,045 to 213,376
_RS00935
Position (kb)
210
211
212 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 209.786 kb on + strand at 209.786 kb on + strand at 209.787 kb on - strand at 210.071 kb on + strand, within AMB_RS00925 at 210.072 kb on - strand, within AMB_RS00925 at 210.072 kb on - strand, within AMB_RS00925 at 210.072 kb on - strand, within AMB_RS00925 at 210.072 kb on - strand, within AMB_RS00925 at 210.072 kb on - strand, within AMB_RS00925 at 210.095 kb on + strand, within AMB_RS00925 at 210.095 kb on + strand, within AMB_RS00925 at 210.096 kb on - strand, within AMB_RS00925 at 210.143 kb on + strand, within AMB_RS00925 at 210.144 kb on - strand, within AMB_RS00925 at 210.404 kb on + strand, within AMB_RS00925 at 210.404 kb on + strand, within AMB_RS00925 at 210.404 kb on + strand, within AMB_RS00925 at 210.404 kb on + strand, within AMB_RS00925 at 210.404 kb on + strand, within AMB_RS00925 at 210.404 kb on + strand, within AMB_RS00925 at 210.404 kb on + strand, within AMB_RS00925 at 210.404 kb on + strand, within AMB_RS00925 at 210.405 kb on - strand, within AMB_RS00925 at 210.405 kb on - strand, within AMB_RS00925 at 210.405 kb on - strand, within AMB_RS00925 at 210.405 kb on - strand, within AMB_RS00925 at 210.405 kb on - strand, within AMB_RS00925 at 210.405 kb on - strand, within AMB_RS00925 at 210.405 kb on - strand, within AMB_RS00925 at 210.440 kb on + strand, within AMB_RS00925 at 210.440 kb on + strand, within AMB_RS00925 at 210.440 kb on + strand, within AMB_RS00925 at 210.440 kb on + strand, within AMB_RS00925 at 210.441 kb on - strand, within AMB_RS00925 at 210.450 kb on - strand, within AMB_RS00925 at 210.883 kb on + strand, within AMB_RS00930 at 211.276 kb on + strand, within AMB_RS00930 at 211.276 kb on + strand, within AMB_RS00930 at 211.300 kb on + strand, within AMB_RS00930 at 211.300 kb on + strand, within AMB_RS00930 at 211.300 kb on + strand, within AMB_RS00930 at 211.301 kb on - strand, within AMB_RS00930 at 211.301 kb on - strand, within AMB_RS00930 at 211.301 kb on - strand, within AMB_RS00930 at 211.459 kb on + strand, within AMB_RS00930 at 211.459 kb on + strand, within AMB_RS00930 at 211.459 kb on + strand, within AMB_RS00930 at 211.459 kb on + strand, within AMB_RS00930 at 211.460 kb on - strand, within AMB_RS00930 at 211.460 kb on - strand, within AMB_RS00930 at 211.460 kb on - strand, within AMB_RS00930 at 211.460 kb on - strand, within AMB_RS00930 at 211.460 kb on - strand, within AMB_RS00930 at 211.460 kb on - strand, within AMB_RS00930 at 211.460 kb on - strand, within AMB_RS00930 at 211.461 kb on + strand, within AMB_RS00930 at 211.474 kb on + strand, within AMB_RS00930 at 211.474 kb on + strand, within AMB_RS00930 at 211.475 kb on - strand, within AMB_RS00930 at 211.475 kb on - strand, within AMB_RS00930 at 211.475 kb on - strand, within AMB_RS00930 at 211.870 kb on + strand at 211.898 kb on - strand at 211.898 kb on - strand at 212.064 kb on + strand at 212.064 kb on + strand at 212.064 kb on + strand at 212.065 kb on - strand at 212.065 kb on - strand at 212.065 kb on - strand at 212.065 kb on - strand at 212.065 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_nonmag_2 remove 209,786 + +1.8 209,786 + -1.9 209,787 - -1.7 210,071 + AMB_RS00925 0.27 +1.8 210,072 - AMB_RS00925 0.27 -3.0 210,072 - AMB_RS00925 0.27 -1.7 210,072 - AMB_RS00925 0.27 -2.4 210,072 - AMB_RS00925 0.27 -2.5 210,072 - AMB_RS00925 0.27 -1.9 210,095 + AMB_RS00925 0.29 -2.9 210,095 + AMB_RS00925 0.29 -0.3 210,096 - AMB_RS00925 0.29 -1.5 210,143 + AMB_RS00925 0.34 -2.2 210,144 - AMB_RS00925 0.34 -0.7 210,404 + AMB_RS00925 0.62 -1.4 210,404 + AMB_RS00925 0.62 -1.6 210,404 + AMB_RS00925 0.62 -1.2 210,404 + AMB_RS00925 0.62 +0.2 210,404 + AMB_RS00925 0.62 -2.4 210,404 + AMB_RS00925 0.62 -0.5 210,404 + AMB_RS00925 0.62 -4.3 210,404 + AMB_RS00925 0.62 +0.8 210,405 - AMB_RS00925 0.63 -0.4 210,405 - AMB_RS00925 0.63 -0.8 210,405 - AMB_RS00925 0.63 -1.6 210,405 - AMB_RS00925 0.63 -2.8 210,405 - AMB_RS00925 0.63 -1.4 210,405 - AMB_RS00925 0.63 -1.1 210,405 - AMB_RS00925 0.63 -1.2 210,440 + AMB_RS00925 0.66 -1.1 210,440 + AMB_RS00925 0.66 -1.7 210,440 + AMB_RS00925 0.66 -1.2 210,440 + AMB_RS00925 0.66 -1.2 210,441 - AMB_RS00925 0.66 -1.8 210,450 - AMB_RS00925 0.67 -1.1 210,883 + AMB_RS00930 0.12 -1.4 211,276 + AMB_RS00930 0.46 +0.2 211,276 + AMB_RS00930 0.46 -3.8 211,300 + AMB_RS00930 0.48 -2.9 211,300 + AMB_RS00930 0.48 -2.5 211,300 + AMB_RS00930 0.48 -0.7 211,301 - AMB_RS00930 0.48 -1.5 211,301 - AMB_RS00930 0.48 -0.8 211,301 - AMB_RS00930 0.48 +0.2 211,459 + AMB_RS00930 0.62 -1.8 211,459 + AMB_RS00930 0.62 -2.4 211,459 + AMB_RS00930 0.62 -1.3 211,459 + AMB_RS00930 0.62 -1.5 211,460 - AMB_RS00930 0.62 -3.4 211,460 - AMB_RS00930 0.62 -1.8 211,460 - AMB_RS00930 0.62 -1.2 211,460 - AMB_RS00930 0.62 -1.4 211,460 - AMB_RS00930 0.62 -1.9 211,460 - AMB_RS00930 0.62 -1.4 211,460 - AMB_RS00930 0.62 -1.5 211,461 + AMB_RS00930 0.62 +0.8 211,474 + AMB_RS00930 0.64 -2.1 211,474 + AMB_RS00930 0.64 -1.0 211,475 - AMB_RS00930 0.64 +1.8 211,475 - AMB_RS00930 0.64 -0.5 211,475 - AMB_RS00930 0.64 +1.8 211,870 + -1.7 211,898 - -0.2 211,898 - -2.2 212,064 + +0.1 212,064 + -0.8 212,064 + +0.2 212,065 - -0.1 212,065 - -2.0 212,065 - -0.4 212,065 - -0.2 212,065 - -1.2
Or see this region's nucleotide sequence