Experiment: 30_an_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS00765 and AMB_RS00770 are separated by 12 nucleotides AMB_RS00770 and AMB_RS00775 are separated by 100 nucleotides AMB_RS00775 and AMB_RS00780 are separated by 38 nucleotides
AMB_RS00765: AMB_RS00765 - glycosyl transferase, at 173,857 to 175,008
_RS00765
AMB_RS00770: AMB_RS00770 - polysaccharide deacetylase family protein, at 175,021 to 175,716
_RS00770
AMB_RS00775: AMB_RS00775 - DUF2478 domain-containing protein, at 175,817 to 177,124
_RS00775
AMB_RS00780: AMB_RS00780 - GTP 3',8-cyclase MoaA, at 177,163 to 178,149
_RS00780
Position (kb)
175
176
177
178 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 175.787 kb on - strand at 175.950 kb on + strand, within AMB_RS00775 at 175.950 kb on + strand, within AMB_RS00775 at 175.950 kb on + strand, within AMB_RS00775 at 175.950 kb on + strand, within AMB_RS00775 at 175.950 kb on + strand, within AMB_RS00775 at 175.951 kb on - strand, within AMB_RS00775 at 175.951 kb on - strand, within AMB_RS00775 at 176.259 kb on + strand, within AMB_RS00775 at 176.260 kb on - strand, within AMB_RS00775 at 176.268 kb on + strand, within AMB_RS00775 at 176.268 kb on + strand, within AMB_RS00775 at 176.269 kb on - strand, within AMB_RS00775 at 176.269 kb on - strand, within AMB_RS00775 at 176.294 kb on + strand, within AMB_RS00775 at 176.295 kb on - strand, within AMB_RS00775 at 176.518 kb on - strand, within AMB_RS00775 at 176.997 kb on + strand at 176.997 kb on + strand at 176.998 kb on - strand at 177.058 kb on + strand at 177.059 kb on - strand at 177.059 kb on - strand at 177.115 kb on + strand at 177.115 kb on + strand at 177.115 kb on + strand at 177.115 kb on + strand at 177.115 kb on + strand at 177.115 kb on + strand at 177.115 kb on + strand at 177.115 kb on + strand at 177.115 kb on + strand at 177.116 kb on - strand at 177.116 kb on - strand at 177.116 kb on - strand at 177.116 kb on - strand at 177.116 kb on - strand at 177.116 kb on - strand at 177.116 kb on - strand at 177.117 kb on + strand at 177.117 kb on + strand at 177.117 kb on + strand at 177.117 kb on + strand at 177.117 kb on + strand at 177.117 kb on + strand at 177.117 kb on + strand at 177.117 kb on + strand at 177.117 kb on + strand at 177.117 kb on + strand at 177.118 kb on - strand at 177.118 kb on - strand at 177.118 kb on - strand at 177.118 kb on - strand at 177.118 kb on - strand at 177.118 kb on - strand at 177.118 kb on - strand at 177.119 kb on + strand at 177.119 kb on + strand at 177.119 kb on + strand at 177.119 kb on + strand at 177.119 kb on + strand at 177.119 kb on + strand at 177.120 kb on - strand at 177.120 kb on - strand at 177.120 kb on - strand at 177.120 kb on - strand at 177.120 kb on - strand at 177.120 kb on - strand at 177.120 kb on - strand at 177.120 kb on - strand at 177.120 kb on - strand at 177.120 kb on - strand at 177.120 kb on - strand at 177.125 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_nonmag_2 remove 175,787 - +0.3 175,950 + AMB_RS00775 0.10 +0.0 175,950 + AMB_RS00775 0.10 -0.8 175,950 + AMB_RS00775 0.10 +1.1 175,950 + AMB_RS00775 0.10 -1.8 175,950 + AMB_RS00775 0.10 +0.2 175,951 - AMB_RS00775 0.10 -1.0 175,951 - AMB_RS00775 0.10 -1.0 176,259 + AMB_RS00775 0.34 +0.3 176,260 - AMB_RS00775 0.34 +0.2 176,268 + AMB_RS00775 0.34 +0.4 176,268 + AMB_RS00775 0.34 +0.2 176,269 - AMB_RS00775 0.35 -3.3 176,269 - AMB_RS00775 0.35 -2.6 176,294 + AMB_RS00775 0.36 -0.2 176,295 - AMB_RS00775 0.37 +0.2 176,518 - AMB_RS00775 0.54 +0.6 176,997 + +0.8 176,997 + +0.1 176,998 - +0.8 177,058 + -0.2 177,059 - -1.2 177,059 - +1.8 177,115 + -0.4 177,115 + -0.9 177,115 + +0.6 177,115 + -0.7 177,115 + -1.9 177,115 + -0.2 177,115 + +0.1 177,115 + -0.5 177,115 + -0.5 177,116 - +0.3 177,116 - +0.4 177,116 - -1.5 177,116 - -1.2 177,116 - +0.2 177,116 - -0.6 177,116 - -0.8 177,117 + -1.9 177,117 + -4.2 177,117 + -0.5 177,117 + +1.8 177,117 + +0.8 177,117 + +0.7 177,117 + -0.6 177,117 + -0.8 177,117 + -0.8 177,117 + -1.9 177,118 - -1.7 177,118 - -1.4 177,118 - -0.0 177,118 - -1.4 177,118 - +0.8 177,118 - -0.1 177,118 - -0.5 177,119 + -2.5 177,119 + -0.8 177,119 + -3.0 177,119 + +1.6 177,119 + +0.2 177,119 + +0.5 177,120 - -0.7 177,120 - -0.7 177,120 - -2.4 177,120 - -1.8 177,120 - -1.3 177,120 - +0.4 177,120 - -1.0 177,120 - +1.0 177,120 - +0.1 177,120 - -2.7 177,120 - +1.7 177,125 + -0.3
Or see this region's nucleotide sequence