Experiment: Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yebY and yebZ are separated by 12 nucleotides yebZ and yobA are separated by 3 nucleotides yobA and holE are separated by 138 nucleotides holE and yobB are separated by 101 nucleotides
b1839: yebY - hypothetical protein (NCBI), at 1,921,389 to 1,921,730
yebY
b1840: yebZ - predicted inner membrane protein (NCBI), at 1,921,743 to 1,922,615
yebZ
b1841: yobA - hypothetical protein (NCBI), at 1,922,619 to 1,922,993
yobA
b1842: holE - DNA polymerase III, theta subunit (NCBI), at 1,923,132 to 1,923,362
holE
b1843: yobB - hypothetical protein (NCBI), at 1,923,464 to 1,924,120
yobB
Position (kb)
1922
1923 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1921.621 kb on + strand, within yebY at 1921.670 kb on + strand, within yebY at 1921.670 kb on + strand, within yebY at 1921.682 kb on + strand, within yebY at 1921.828 kb on + strand at 1921.828 kb on + strand at 1921.828 kb on + strand at 1921.828 kb on + strand at 1921.846 kb on + strand, within yebZ at 1922.093 kb on - strand, within yebZ at 1922.107 kb on - strand, within yebZ at 1922.363 kb on - strand, within yebZ at 1922.363 kb on - strand, within yebZ at 1922.430 kb on + strand, within yebZ at 1922.430 kb on + strand, within yebZ at 1922.438 kb on + strand, within yebZ at 1922.446 kb on - strand, within yebZ at 1922.482 kb on - strand, within yebZ at 1922.528 kb on + strand at 1922.536 kb on - strand at 1922.642 kb on - strand at 1922.681 kb on + strand, within yobA at 1922.681 kb on + strand, within yobA at 1923.028 kb on + strand at 1923.059 kb on - strand at 1923.246 kb on - strand, within holE at 1923.246 kb on - strand, within holE at 1923.296 kb on + strand, within holE at 1923.364 kb on + strand at 1923.364 kb on + strand at 1923.493 kb on + strand at 1923.493 kb on + strand at 1923.666 kb on - strand, within yobB at 1923.831 kb on - strand, within yobB at 1923.840 kb on - strand, within yobB at 1923.840 kb on - strand, within yobB at 1923.846 kb on + strand, within yobB
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 7.5 vol% remove 1,921,621 + yebY b1839 0.68 +0.7 1,921,670 + yebY b1839 0.82 +1.6 1,921,670 + yebY b1839 0.82 -0.3 1,921,682 + yebY b1839 0.86 -0.6 1,921,828 + +0.7 1,921,828 + +0.7 1,921,828 + -2.3 1,921,828 + +0.7 1,921,846 + yebZ b1840 0.12 -1.1 1,922,093 - yebZ b1840 0.40 -0.3 1,922,107 - yebZ b1840 0.42 -0.3 1,922,363 - yebZ b1840 0.71 +0.6 1,922,363 - yebZ b1840 0.71 +0.4 1,922,430 + yebZ b1840 0.79 +0.7 1,922,430 + yebZ b1840 0.79 +0.5 1,922,438 + yebZ b1840 0.80 -0.1 1,922,446 - yebZ b1840 0.81 -0.8 1,922,482 - yebZ b1840 0.85 -0.5 1,922,528 + +0.8 1,922,536 - -0.7 1,922,642 - -0.1 1,922,681 + yobA b1841 0.17 +0.3 1,922,681 + yobA b1841 0.17 -0.8 1,923,028 + +0.5 1,923,059 - +0.2 1,923,246 - holE b1842 0.49 -0.2 1,923,246 - holE b1842 0.49 -0.8 1,923,296 + holE b1842 0.71 +0.3 1,923,364 + -0.2 1,923,364 + +0.3 1,923,493 + +2.1 1,923,493 + +1.0 1,923,666 - yobB b1843 0.31 +0.8 1,923,831 - yobB b1843 0.56 -0.7 1,923,840 - yobB b1843 0.57 +1.2 1,923,840 - yobB b1843 0.57 +1.8 1,923,846 + yobB b1843 0.58 +0.3
Or see this region's nucleotide sequence