Experiment: Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt b1481 and osmC are separated by 344 nucleotides osmC and ddpF are separated by 55 nucleotides ddpF and ddpD overlap by 8 nucleotides ddpD and ddpC overlap by 4 nucleotides
b1481: b1481 - orf, hypothetical protein (VIMSS), at 1,554,089 to 1,554,304
b1481
b1482: osmC - osmotically inducible, stress-inducible membrane protein (NCBI), at 1,554,649 to 1,555,080
osmC
b1483: ddpF - D-ala-D-ala transporter subunit (NCBI), at 1,555,136 to 1,556,062
ddpF
b1484: ddpD - D-ala-D-ala transporter subunit (NCBI), at 1,556,055 to 1,557,041
ddpD
b1485: ddpC - D-ala-D-ala transporter subunit (NCBI), at 1,557,038 to 1,557,934
ddpC
Position (kb)
1555
1556
1557 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1554.144 kb on - strand, within b1481 at 1554.218 kb on + strand, within b1481 at 1554.224 kb on - strand, within b1481 at 1554.224 kb on - strand, within b1481 at 1554.244 kb on - strand, within b1481 at 1554.249 kb on + strand, within b1481 at 1554.257 kb on - strand, within b1481 at 1554.273 kb on + strand, within b1481 at 1554.365 kb on + strand at 1554.407 kb on + strand at 1554.599 kb on + strand at 1554.599 kb on + strand at 1554.601 kb on + strand at 1554.639 kb on - strand at 1554.678 kb on + strand at 1554.678 kb on - strand at 1554.684 kb on - strand at 1554.697 kb on + strand, within osmC at 1554.707 kb on + strand, within osmC at 1554.718 kb on + strand, within osmC at 1554.723 kb on - strand, within osmC at 1555.296 kb on + strand, within ddpF at 1555.384 kb on - strand, within ddpF at 1555.384 kb on - strand, within ddpF at 1555.388 kb on + strand, within ddpF at 1555.388 kb on + strand, within ddpF at 1555.544 kb on - strand, within ddpF at 1555.722 kb on + strand, within ddpF at 1555.722 kb on + strand, within ddpF at 1555.874 kb on - strand, within ddpF at 1555.874 kb on - strand, within ddpF at 1556.155 kb on - strand, within ddpD at 1556.213 kb on - strand, within ddpD at 1556.230 kb on + strand, within ddpD at 1556.230 kb on + strand, within ddpD at 1556.320 kb on - strand, within ddpD at 1556.320 kb on - strand, within ddpD at 1556.375 kb on + strand, within ddpD at 1556.389 kb on + strand, within ddpD at 1556.723 kb on + strand, within ddpD at 1556.725 kb on - strand, within ddpD
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 7.5 vol% remove 1,554,144 - b1481 b1481 0.25 +1.2 1,554,218 + b1481 b1481 0.60 +1.8 1,554,224 - b1481 b1481 0.62 -1.1 1,554,224 - b1481 b1481 0.62 +1.1 1,554,244 - b1481 b1481 0.72 +0.2 1,554,249 + b1481 b1481 0.74 -0.1 1,554,257 - b1481 b1481 0.78 -0.8 1,554,273 + b1481 b1481 0.85 +0.4 1,554,365 + -0.1 1,554,407 + -0.3 1,554,599 + +0.7 1,554,599 + +0.2 1,554,601 + +2.0 1,554,639 - +0.7 1,554,678 + +0.1 1,554,678 - +0.1 1,554,684 - +0.3 1,554,697 + osmC b1482 0.11 +1.0 1,554,707 + osmC b1482 0.13 -2.5 1,554,718 + osmC b1482 0.16 +0.1 1,554,723 - osmC b1482 0.17 +0.2 1,555,296 + ddpF b1483 0.17 +1.4 1,555,384 - ddpF b1483 0.27 +0.5 1,555,384 - ddpF b1483 0.27 -0.2 1,555,388 + ddpF b1483 0.27 -0.6 1,555,388 + ddpF b1483 0.27 +0.2 1,555,544 - ddpF b1483 0.44 +1.1 1,555,722 + ddpF b1483 0.63 +0.4 1,555,722 + ddpF b1483 0.63 -0.2 1,555,874 - ddpF b1483 0.80 +0.4 1,555,874 - ddpF b1483 0.80 +1.7 1,556,155 - ddpD b1484 0.10 +1.1 1,556,213 - ddpD b1484 0.16 -0.1 1,556,230 + ddpD b1484 0.18 +0.7 1,556,230 + ddpD b1484 0.18 -0.9 1,556,320 - ddpD b1484 0.27 +0.2 1,556,320 - ddpD b1484 0.27 +0.2 1,556,375 + ddpD b1484 0.32 +0.4 1,556,389 + ddpD b1484 0.34 -0.8 1,556,723 + ddpD b1484 0.68 +0.1 1,556,725 - ddpD b1484 0.68 -0.3
Or see this region's nucleotide sequence