Experiment: Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt paaE and paaF overlap by 4 nucleotides paaF and paaG overlap by 1 nucleotides paaG and paaH are separated by 1 nucleotides
b1392: paaE - predicted multicomponent oxygenase/reductase subunit for phenylacetic acid degradation (NCBI), at 1,454,454 to 1,455,524
paaE
b1393: paaF - enoyl-CoA hydratase-isomerase (NCBI), at 1,455,521 to 1,456,288
paaF
b1394: paaG - enoyl-CoA hydratase (NCBI), at 1,456,288 to 1,457,076
paaG
b1395: paaH - 3-hydroxybutyryl-CoA dehydrogenase (NCBI), at 1,457,078 to 1,458,505
paaH
Position (kb)
1456
1457
1458 Strain fitness (log2 ratio)
-2
-1
0
1 at 1455.315 kb on + strand, within paaE at 1455.392 kb on - strand, within paaE at 1455.429 kb on - strand at 1455.610 kb on + strand, within paaF at 1456.073 kb on + strand, within paaF at 1456.216 kb on - strand at 1456.216 kb on - strand at 1456.490 kb on + strand, within paaG at 1456.637 kb on + strand, within paaG at 1456.780 kb on - strand, within paaG at 1456.910 kb on + strand, within paaG at 1457.029 kb on + strand at 1457.029 kb on + strand at 1457.192 kb on + strand at 1457.264 kb on + strand, within paaH at 1457.625 kb on + strand, within paaH at 1457.625 kb on + strand, within paaH at 1457.668 kb on + strand, within paaH at 1457.750 kb on + strand, within paaH at 1457.822 kb on + strand, within paaH at 1457.921 kb on - strand, within paaH at 1457.921 kb on - strand, within paaH at 1457.941 kb on + strand, within paaH at 1457.979 kb on + strand, within paaH at 1458.061 kb on - strand, within paaH at 1458.061 kb on - strand, within paaH
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 7.5 vol% remove 1,455,315 + paaE b1392 0.80 -1.5 1,455,392 - paaE b1392 0.88 +0.4 1,455,429 - +0.7 1,455,610 + paaF b1393 0.12 -0.3 1,456,073 + paaF b1393 0.72 -0.6 1,456,216 - +1.7 1,456,216 - +1.1 1,456,490 + paaG b1394 0.26 -0.5 1,456,637 + paaG b1394 0.44 -0.3 1,456,780 - paaG b1394 0.62 +0.2 1,456,910 + paaG b1394 0.79 -2.3 1,457,029 + -0.1 1,457,029 + -0.3 1,457,192 + +1.0 1,457,264 + paaH b1395 0.13 +0.1 1,457,625 + paaH b1395 0.38 -0.2 1,457,625 + paaH b1395 0.38 +0.5 1,457,668 + paaH b1395 0.41 +0.7 1,457,750 + paaH b1395 0.47 -0.0 1,457,822 + paaH b1395 0.52 -0.1 1,457,921 - paaH b1395 0.59 +0.3 1,457,921 - paaH b1395 0.59 -0.0 1,457,941 + paaH b1395 0.60 -0.6 1,457,979 + paaH b1395 0.63 +1.7 1,458,061 - paaH b1395 0.69 +0.9 1,458,061 - paaH b1395 0.69 -0.6
Or see this region's nucleotide sequence