Experiment: Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rluC and yceF are separated by 111 nucleotides yceF and sraB overlap by 7 nucleotides sraB and yceD are separated by 36 nucleotides yceD and rpmF are separated by 51 nucleotides rpmF and plsX are separated by 80 nucleotides
b1086: rluC - 23S rRNA pseudouridylate synthase (NCBI), at 1,144,163 to 1,145,122
rluC
b1087: yceF - orf, hypothetical protein (VIMSS), at 1,145,234 to 1,145,818
yceF
b4418: sraB - unknown RNA (NCBI), at 1,145,812 to 1,145,980
sraB
b1088: yceD - hypothetical protein (NCBI), at 1,146,017 to 1,146,538
yceD
b1089: rpmF - 50S ribosomal protein L32 (NCBI), at 1,146,590 to 1,146,763
rpmF
b1090: plsX - glycerolphosphate auxotrophy in plsB background (VIMSS), at 1,146,844 to 1,147,914
plsX
Position (kb)
1146
1147 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1145.035 kb on + strand at 1145.058 kb on - strand at 1145.145 kb on - strand at 1145.279 kb on - strand at 1145.381 kb on + strand, within yceF at 1145.381 kb on + strand, within yceF at 1145.381 kb on + strand, within yceF at 1145.381 kb on + strand, within yceF at 1145.389 kb on - strand, within yceF at 1145.389 kb on - strand, within yceF at 1145.389 kb on - strand, within yceF at 1145.395 kb on + strand, within yceF at 1145.511 kb on - strand, within yceF at 1145.516 kb on - strand, within yceF at 1145.516 kb on - strand, within yceF at 1145.576 kb on - strand, within yceF at 1145.610 kb on - strand, within yceF at 1145.614 kb on - strand, within yceF at 1145.673 kb on - strand, within yceF at 1145.724 kb on - strand, within yceF at 1145.734 kb on - strand, within yceF at 1145.734 kb on - strand, within yceF at 1145.841 kb on + strand, within sraB at 1145.847 kb on - strand, within sraB at 1146.042 kb on + strand at 1146.052 kb on + strand at 1146.096 kb on + strand, within yceD at 1146.096 kb on + strand, within yceD at 1146.117 kb on + strand, within yceD at 1146.265 kb on + strand, within yceD at 1146.265 kb on + strand, within yceD at 1146.265 kb on + strand, within yceD at 1146.265 kb on + strand, within yceD at 1146.277 kb on + strand, within yceD at 1146.277 kb on + strand, within yceD at 1146.310 kb on + strand, within yceD at 1146.373 kb on + strand, within yceD at 1146.373 kb on + strand, within yceD at 1146.987 kb on + strand, within plsX at 1147.009 kb on - strand, within plsX at 1147.019 kb on - strand, within plsX at 1147.019 kb on - strand, within plsX at 1147.059 kb on + strand, within plsX at 1147.130 kb on - strand, within plsX at 1147.298 kb on + strand, within plsX at 1147.298 kb on + strand, within plsX at 1147.385 kb on - strand, within plsX at 1147.385 kb on - strand, within plsX at 1147.429 kb on + strand, within plsX
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 7.5 vol% remove 1,145,035 + +0.5 1,145,058 - -0.2 1,145,145 - +0.3 1,145,279 - +0.1 1,145,381 + yceF b1087 0.25 +0.3 1,145,381 + yceF b1087 0.25 -0.4 1,145,381 + yceF b1087 0.25 -0.9 1,145,381 + yceF b1087 0.25 +0.6 1,145,389 - yceF b1087 0.26 +2.4 1,145,389 - yceF b1087 0.26 +1.8 1,145,389 - yceF b1087 0.26 -0.8 1,145,395 + yceF b1087 0.28 +0.5 1,145,511 - yceF b1087 0.47 +0.6 1,145,516 - yceF b1087 0.48 +2.4 1,145,516 - yceF b1087 0.48 -0.2 1,145,576 - yceF b1087 0.58 -0.1 1,145,610 - yceF b1087 0.64 +0.2 1,145,614 - yceF b1087 0.65 -0.1 1,145,673 - yceF b1087 0.75 +2.4 1,145,724 - yceF b1087 0.84 +1.2 1,145,734 - yceF b1087 0.85 -0.4 1,145,734 - yceF b1087 0.85 -0.2 1,145,841 + sraB b4418 0.17 +0.9 1,145,847 - sraB b4418 0.21 -0.8 1,146,042 + +0.1 1,146,052 + +2.4 1,146,096 + yceD b1088 0.15 -0.2 1,146,096 + yceD b1088 0.15 -0.8 1,146,117 + yceD b1088 0.19 -1.2 1,146,265 + yceD b1088 0.48 -1.4 1,146,265 + yceD b1088 0.48 +0.8 1,146,265 + yceD b1088 0.48 +0.1 1,146,265 + yceD b1088 0.48 -1.7 1,146,277 + yceD b1088 0.50 +0.8 1,146,277 + yceD b1088 0.50 +0.1 1,146,310 + yceD b1088 0.56 -2.0 1,146,373 + yceD b1088 0.68 -0.2 1,146,373 + yceD b1088 0.68 +3.4 1,146,987 + plsX b1090 0.13 +0.8 1,147,009 - plsX b1090 0.15 +0.9 1,147,019 - plsX b1090 0.16 +1.2 1,147,019 - plsX b1090 0.16 -0.2 1,147,059 + plsX b1090 0.20 -1.5 1,147,130 - plsX b1090 0.27 -0.8 1,147,298 + plsX b1090 0.42 +1.0 1,147,298 + plsX b1090 0.42 +0.8 1,147,385 - plsX b1090 0.51 -0.1 1,147,385 - plsX b1090 0.51 +0.2 1,147,429 + plsX b1090 0.55 +1.1
Or see this region's nucleotide sequence