Experiment: Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tauA and tauB are separated by 12 nucleotides tauB and tauC overlap by 4 nucleotides tauC and tauD overlap by 4 nucleotides tauD and hemB are separated by 106 nucleotides
b0365: tauA - taurine transport system periplasmic protein (VIMSS), at 384,456 to 385,418
tauA
b0366: tauB - taurine transporter subunit (NCBI), at 385,431 to 386,198
tauB
b0367: tauC - taurine transporter subunit (NCBI), at 386,195 to 387,022
tauC
b0368: tauD - taurine dioxygenase (NCBI), at 387,019 to 387,870
tauD
b0369: hemB - 5-aminolevulinate dehydratase = porphobilinogen synthase (VIMSS), at 387,977 to 388,951
hemB
Position (kb) 386
387
388 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 385.340 kb on + strand at 385.340 kb on + strand at 385.362 kb on + strand at 385.514 kb on + strand, within tauB at 385.514 kb on + strand, within tauB at 385.523 kb on - strand, within tauB at 385.523 kb on - strand, within tauB at 385.571 kb on + strand, within tauB at 385.571 kb on + strand, within tauB at 385.609 kb on - strand, within tauB at 385.687 kb on + strand, within tauB at 385.692 kb on + strand, within tauB at 385.817 kb on + strand, within tauB at 385.817 kb on + strand, within tauB at 385.868 kb on - strand, within tauB at 385.875 kb on - strand, within tauB at 385.875 kb on - strand, within tauB at 385.926 kb on - strand, within tauB at 385.974 kb on + strand, within tauB at 385.993 kb on + strand, within tauB at 385.999 kb on - strand, within tauB at 386.055 kb on + strand, within tauB at 386.094 kb on + strand, within tauB at 386.245 kb on - strand at 386.275 kb on + strand at 386.297 kb on - strand, within tauC at 386.297 kb on - strand, within tauC at 386.532 kb on + strand, within tauC at 386.532 kb on + strand, within tauC at 386.562 kb on + strand, within tauC at 386.586 kb on - strand, within tauC at 386.729 kb on + strand, within tauC at 386.829 kb on - strand, within tauC at 386.958 kb on + strand at 387.043 kb on - strand at 387.266 kb on + strand, within tauD at 387.266 kb on + strand, within tauD at 387.410 kb on + strand, within tauD at 387.622 kb on + strand, within tauD at 387.649 kb on + strand, within tauD at 387.649 kb on + strand, within tauD at 387.669 kb on - strand, within tauD at 387.678 kb on + strand, within tauD at 387.775 kb on + strand, within tauD at 387.775 kb on + strand, within tauD at 387.783 kb on - strand, within tauD at 387.896 kb on + strand at 387.908 kb on + strand at 387.908 kb on + strand at 387.916 kb on - strand at 387.916 kb on - strand at 387.916 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 7.5 vol% remove 385,340 + +1.0 385,340 + -1.8 385,362 + +0.8 385,514 + tauB b0366 0.11 -0.6 385,514 + tauB b0366 0.11 +1.0 385,523 - tauB b0366 0.12 +0.4 385,523 - tauB b0366 0.12 +1.0 385,571 + tauB b0366 0.18 +0.6 385,571 + tauB b0366 0.18 -3.0 385,609 - tauB b0366 0.23 +1.0 385,687 + tauB b0366 0.33 +0.6 385,692 + tauB b0366 0.34 +0.3 385,817 + tauB b0366 0.50 +1.6 385,817 + tauB b0366 0.50 +0.4 385,868 - tauB b0366 0.57 +0.6 385,875 - tauB b0366 0.58 +1.2 385,875 - tauB b0366 0.58 -1.6 385,926 - tauB b0366 0.64 +0.8 385,974 + tauB b0366 0.71 -0.4 385,993 + tauB b0366 0.73 +1.0 385,999 - tauB b0366 0.74 +0.4 386,055 + tauB b0366 0.81 +0.3 386,094 + tauB b0366 0.86 -0.2 386,245 - +0.4 386,275 + +0.5 386,297 - tauC b0367 0.12 -0.6 386,297 - tauC b0367 0.12 +1.0 386,532 + tauC b0367 0.41 -0.4 386,532 + tauC b0367 0.41 -1.1 386,562 + tauC b0367 0.44 -0.4 386,586 - tauC b0367 0.47 -1.8 386,729 + tauC b0367 0.64 +0.6 386,829 - tauC b0367 0.77 -0.3 386,958 + +0.2 387,043 - +2.0 387,266 + tauD b0368 0.29 -0.4 387,266 + tauD b0368 0.29 +2.3 387,410 + tauD b0368 0.46 +0.9 387,622 + tauD b0368 0.71 +0.4 387,649 + tauD b0368 0.74 +2.0 387,649 + tauD b0368 0.74 +1.0 387,669 - tauD b0368 0.76 +1.7 387,678 + tauD b0368 0.77 +1.0 387,775 + tauD b0368 0.89 +1.4 387,775 + tauD b0368 0.89 -0.4 387,783 - tauD b0368 0.90 -0.0 387,896 + -0.0 387,908 + -0.0 387,908 + -0.3 387,916 - -0.0 387,916 - -0.0 387,916 - +0.3
Or see this region's nucleotide sequence