Experiment: Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ilvI and ilvH are separated by 2 nucleotides ilvH and fruR are separated by 179 nucleotides fruR and mraZ are separated by 601 nucleotides
b0077: ilvI - acetolactate synthase III large subunit (NCBI), at 85,630 to 87,354
ilvI
b0078: ilvH - acetolactate synthase small subunit (NCBI), at 87,357 to 87,848
ilvH
b0080: fruR - DNA-binding transcriptional dual regulator (NCBI), at 88,028 to 89,032
fruR
b0081: mraZ - hypothetical protein (NCBI), at 89,634 to 90,092
mraZ
Position (kb)
88
89
90 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 87.034 kb on - strand, within ilvI at 87.062 kb on - strand, within ilvI at 87.070 kb on + strand, within ilvI at 87.070 kb on + strand, within ilvI at 87.092 kb on - strand, within ilvI at 87.098 kb on - strand, within ilvI at 87.098 kb on - strand, within ilvI at 87.130 kb on + strand, within ilvI at 87.165 kb on - strand, within ilvI at 87.166 kb on - strand, within ilvI at 87.250 kb on + strand at 87.285 kb on + strand at 87.443 kb on - strand, within ilvH at 87.480 kb on + strand, within ilvH at 87.497 kb on + strand, within ilvH at 87.571 kb on + strand, within ilvH at 87.571 kb on + strand, within ilvH at 87.588 kb on - strand, within ilvH at 87.617 kb on - strand, within ilvH at 87.617 kb on - strand, within ilvH at 87.640 kb on + strand, within ilvH at 87.665 kb on - strand, within ilvH at 87.698 kb on - strand, within ilvH at 87.705 kb on + strand, within ilvH at 87.710 kb on + strand, within ilvH at 87.710 kb on + strand, within ilvH at 87.718 kb on - strand, within ilvH at 87.718 kb on - strand, within ilvH at 87.718 kb on - strand, within ilvH at 87.718 kb on - strand, within ilvH at 87.740 kb on - strand, within ilvH at 87.744 kb on - strand, within ilvH at 87.785 kb on + strand, within ilvH at 87.802 kb on + strand at 87.815 kb on - strand at 87.918 kb on - strand at 87.951 kb on - strand at 87.955 kb on + strand at 88.009 kb on + strand at 88.074 kb on + strand at 88.085 kb on + strand at 88.111 kb on - strand at 88.111 kb on - strand at 88.111 kb on - strand at 88.111 kb on - strand at 88.113 kb on - strand at 88.187 kb on + strand, within fruR at 88.187 kb on + strand, within fruR at 88.259 kb on - strand, within fruR at 88.265 kb on + strand, within fruR at 88.365 kb on + strand, within fruR at 88.365 kb on + strand, within fruR at 88.432 kb on + strand, within fruR at 88.432 kb on + strand, within fruR at 88.471 kb on - strand, within fruR at 88.482 kb on + strand, within fruR at 88.490 kb on - strand, within fruR at 88.492 kb on + strand, within fruR at 88.492 kb on + strand, within fruR at 88.492 kb on + strand, within fruR at 88.500 kb on - strand, within fruR at 88.517 kb on - strand, within fruR at 88.517 kb on - strand, within fruR at 88.683 kb on + strand, within fruR at 88.753 kb on + strand, within fruR at 88.772 kb on + strand at 88.774 kb on + strand, within fruR at 88.821 kb on + strand, within fruR at 88.851 kb on + strand, within fruR at 88.851 kb on + strand, within fruR at 88.868 kb on + strand, within fruR at 88.894 kb on + strand, within fruR at 88.937 kb on + strand at 88.945 kb on - strand at 88.945 kb on - strand at 88.946 kb on + strand at 88.949 kb on - strand at 88.992 kb on + strand at 89.000 kb on + strand at 89.001 kb on - strand at 89.012 kb on + strand at 89.015 kb on + strand at 89.015 kb on + strand at 89.017 kb on + strand at 89.022 kb on + strand at 89.023 kb on - strand at 89.023 kb on - strand at 89.023 kb on - strand at 89.079 kb on + strand at 89.079 kb on + strand at 89.079 kb on + strand at 89.079 kb on + strand at 89.079 kb on + strand at 89.079 kb on + strand at 89.086 kb on + strand at 89.087 kb on - strand at 89.087 kb on - strand at 89.087 kb on - strand at 89.087 kb on - strand at 89.087 kb on - strand at 89.087 kb on - strand at 89.089 kb on - strand at 89.089 kb on - strand at 89.089 kb on - strand at 89.089 kb on - strand at 89.091 kb on + strand at 89.122 kb on + strand at 89.132 kb on + strand at 89.225 kb on + strand at 89.294 kb on - strand at 89.307 kb on + strand at 89.307 kb on + strand at 89.312 kb on + strand at 89.312 kb on + strand at 89.312 kb on + strand at 89.313 kb on - strand at 89.315 kb on - strand at 89.325 kb on + strand at 89.325 kb on - strand at 89.330 kb on - strand at 89.391 kb on + strand at 89.393 kb on + strand at 89.401 kb on - strand at 89.411 kb on + strand at 89.411 kb on + strand at 89.416 kb on - strand at 89.508 kb on + strand at 89.508 kb on + strand at 89.542 kb on - strand at 89.542 kb on - strand at 89.550 kb on + strand at 89.558 kb on - strand at 89.649 kb on + strand at 89.649 kb on + strand at 89.658 kb on + strand at 89.680 kb on + strand, within mraZ at 89.685 kb on + strand, within mraZ at 89.917 kb on + strand, within mraZ at 89.917 kb on + strand, within mraZ at 89.981 kb on + strand, within mraZ
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 7.5 vol% remove 87,034 - ilvI b0077 0.81 -0.7 87,062 - ilvI b0077 0.83 -0.1 87,070 + ilvI b0077 0.83 +0.1 87,070 + ilvI b0077 0.83 +0.2 87,092 - ilvI b0077 0.85 -0.5 87,098 - ilvI b0077 0.85 +0.5 87,098 - ilvI b0077 0.85 +0.2 87,130 + ilvI b0077 0.87 -0.6 87,165 - ilvI b0077 0.89 +1.0 87,166 - ilvI b0077 0.89 -0.6 87,250 + +1.0 87,285 + +0.4 87,443 - ilvH b0078 0.17 +1.0 87,480 + ilvH b0078 0.25 +0.3 87,497 + ilvH b0078 0.28 -0.2 87,571 + ilvH b0078 0.43 +0.0 87,571 + ilvH b0078 0.43 +0.3 87,588 - ilvH b0078 0.47 +0.3 87,617 - ilvH b0078 0.53 +0.9 87,617 - ilvH b0078 0.53 +1.0 87,640 + ilvH b0078 0.58 +0.1 87,665 - ilvH b0078 0.63 +0.2 87,698 - ilvH b0078 0.69 -1.6 87,705 + ilvH b0078 0.71 -0.4 87,710 + ilvH b0078 0.72 +0.8 87,710 + ilvH b0078 0.72 +0.3 87,718 - ilvH b0078 0.73 -0.1 87,718 - ilvH b0078 0.73 +0.9 87,718 - ilvH b0078 0.73 +0.6 87,718 - ilvH b0078 0.73 -0.6 87,740 - ilvH b0078 0.78 +0.2 87,744 - ilvH b0078 0.79 -2.7 87,785 + ilvH b0078 0.87 +0.4 87,802 + -0.7 87,815 - -1.6 87,918 - -0.9 87,951 - +0.4 87,955 + +0.0 88,009 + -0.1 88,074 + +0.6 88,085 + +0.0 88,111 - -1.0 88,111 - +2.0 88,111 - -0.3 88,111 - +0.2 88,113 - +0.0 88,187 + fruR b0080 0.16 -0.3 88,187 + fruR b0080 0.16 +0.0 88,259 - fruR b0080 0.23 +0.2 88,265 + fruR b0080 0.24 -2.0 88,365 + fruR b0080 0.34 -1.3 88,365 + fruR b0080 0.34 +0.0 88,432 + fruR b0080 0.40 +1.4 88,432 + fruR b0080 0.40 -1.2 88,471 - fruR b0080 0.44 -0.6 88,482 + fruR b0080 0.45 -0.6 88,490 - fruR b0080 0.46 -0.4 88,492 + fruR b0080 0.46 -0.6 88,492 + fruR b0080 0.46 -0.6 88,492 + fruR b0080 0.46 +0.7 88,500 - fruR b0080 0.47 +0.2 88,517 - fruR b0080 0.49 +3.3 88,517 - fruR b0080 0.49 -1.0 88,683 + fruR b0080 0.65 -1.1 88,753 + fruR b0080 0.72 +0.4 88,772 + +1.0 88,774 + fruR b0080 0.74 +0.4 88,821 + fruR b0080 0.79 -2.2 88,851 + fruR b0080 0.82 +0.0 88,851 + fruR b0080 0.82 +1.0 88,868 + fruR b0080 0.84 -1.0 88,894 + fruR b0080 0.86 -1.3 88,937 + +0.6 88,945 - -2.3 88,945 - -1.3 88,946 + -0.2 88,949 - -1.4 88,992 + +0.1 89,000 + -0.5 89,001 - +0.6 89,012 + +0.0 89,015 + -0.4 89,015 + -0.4 89,017 + -0.6 89,022 + +0.8 89,023 - -0.4 89,023 - -0.3 89,023 - -0.1 89,079 + +0.8 89,079 + +0.9 89,079 + -0.5 89,079 + +0.4 89,079 + +0.4 89,079 + +0.7 89,086 + -0.6 89,087 - -1.8 89,087 - +0.3 89,087 - +0.5 89,087 - -1.3 89,087 - -1.0 89,087 - +0.4 89,089 - -1.3 89,089 - +1.2 89,089 - +0.0 89,089 - -1.0 89,091 + -0.2 89,122 + +0.6 89,132 + +0.1 89,225 + -0.4 89,294 - -0.1 89,307 + +0.0 89,307 + +1.0 89,312 + +0.0 89,312 + +0.1 89,312 + -0.2 89,313 - +1.3 89,315 - +0.9 89,325 + -0.0 89,325 - +0.2 89,330 - +1.0 89,391 + +0.0 89,393 + +0.2 89,401 - -0.0 89,411 + +0.3 89,411 + +1.4 89,416 - +0.0 89,508 + -0.6 89,508 + +0.7 89,542 - -0.8 89,542 - +0.0 89,550 + +0.0 89,558 - +0.3 89,649 + -0.8 89,649 + -0.1 89,658 + +1.6 89,680 + mraZ b0081 0.10 +0.0 89,685 + mraZ b0081 0.11 +0.6 89,917 + mraZ b0081 0.62 +2.6 89,917 + mraZ b0081 0.62 -1.0 89,981 + mraZ b0081 0.76 -0.6
Or see this region's nucleotide sequence