Experiment: 30_an_nonmag_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS23635 and AMB_RS20480 are separated by 28 nucleotides AMB_RS20480 and AMB_RS20485 overlap by 14 nucleotides AMB_RS20485 and AMB_RS20490 are separated by 8 nucleotides AMB_RS20490 and AMB_RS20495 are separated by 119 nucleotides AMB_RS20495 and AMB_RS20500 are separated by 163 nucleotides AMB_RS20500 and AMB_RS23640 are separated by 123 nucleotides
AMB_RS23635: AMB_RS23635 - hypothetical protein, at 4,449,238 to 4,449,720
_RS23635
AMB_RS20480: AMB_RS20480 - hypothetical protein, at 4,449,749 to 4,450,054
_RS20480
AMB_RS20485: AMB_RS20485 - hypothetical protein, at 4,450,041 to 4,450,238
_RS20485
AMB_RS20490: AMB_RS20490 - hypothetical protein, at 4,450,247 to 4,450,510
_RS20490
AMB_RS20495: AMB_RS20495 - helix-turn-helix domain-containing protein, at 4,450,630 to 4,450,812
_RS20495
AMB_RS20500: AMB_RS20500 - tRNA-Gly, at 4,450,976 to 4,451,049
_RS20500
AMB_RS23640: AMB_RS23640 - hypothetical protein, at 4,451,173 to 4,454,229
_RS23640
Position (kb)
4450
4451 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4449.794 kb on + strand, within AMB_RS20480 at 4449.794 kb on + strand, within AMB_RS20480 at 4449.794 kb on + strand, within AMB_RS20480 at 4449.794 kb on + strand, within AMB_RS20480 at 4449.795 kb on - strand, within AMB_RS20480 at 4449.795 kb on - strand, within AMB_RS20480 at 4449.795 kb on - strand, within AMB_RS20480 at 4449.795 kb on - strand, within AMB_RS20480 at 4449.857 kb on + strand, within AMB_RS20480 at 4449.858 kb on - strand, within AMB_RS20480 at 4449.914 kb on + strand, within AMB_RS20480 at 4450.475 kb on + strand, within AMB_RS20490 at 4450.475 kb on + strand, within AMB_RS20490 at 4450.476 kb on - strand, within AMB_RS20490 at 4450.559 kb on + strand at 4450.559 kb on + strand at 4450.559 kb on + strand at 4450.648 kb on + strand at 4450.648 kb on + strand at 4450.648 kb on + strand at 4450.648 kb on + strand at 4450.648 kb on + strand at 4450.648 kb on + strand at 4450.648 kb on + strand at 4450.648 kb on + strand at 4450.649 kb on - strand, within AMB_RS20495 at 4450.649 kb on - strand, within AMB_RS20495 at 4450.649 kb on - strand, within AMB_RS20495 at 4450.649 kb on - strand, within AMB_RS20495 at 4450.843 kb on + strand at 4450.843 kb on + strand at 4450.844 kb on - strand at 4450.844 kb on - strand at 4450.844 kb on - strand at 4450.884 kb on + strand at 4450.884 kb on + strand at 4450.885 kb on - strand at 4450.893 kb on - strand at 4451.041 kb on + strand, within AMB_RS20500 at 4451.182 kb on + strand at 4451.182 kb on + strand at 4451.182 kb on + strand at 4451.182 kb on + strand at 4451.253 kb on + strand at 4451.253 kb on + strand at 4451.254 kb on - strand at 4451.254 kb on - strand at 4451.384 kb on + strand at 4451.384 kb on + strand at 4451.385 kb on - strand at 4451.385 kb on - strand at 4451.385 kb on - strand at 4451.385 kb on - strand at 4451.457 kb on + strand at 4451.457 kb on + strand at 4451.457 kb on + strand at 4451.457 kb on + strand at 4451.458 kb on - strand at 4451.458 kb on - strand at 4451.458 kb on - strand at 4451.547 kb on + strand, within AMB_RS23640 at 4451.547 kb on + strand, within AMB_RS23640 at 4451.547 kb on + strand, within AMB_RS23640 at 4451.547 kb on + strand, within AMB_RS23640 at 4451.548 kb on - strand, within AMB_RS23640 at 4451.548 kb on - strand, within AMB_RS23640 at 4451.548 kb on - strand, within AMB_RS23640 at 4451.548 kb on - strand, within AMB_RS23640 at 4451.548 kb on - strand, within AMB_RS23640 at 4451.625 kb on + strand, within AMB_RS23640 at 4451.625 kb on + strand, within AMB_RS23640 at 4451.626 kb on - strand, within AMB_RS23640 at 4451.626 kb on - strand, within AMB_RS23640 at 4451.626 kb on - strand, within AMB_RS23640 at 4451.675 kb on + strand, within AMB_RS23640 at 4451.676 kb on - strand, within AMB_RS23640 at 4451.676 kb on - strand, within AMB_RS23640 at 4451.676 kb on - strand, within AMB_RS23640 at 4451.676 kb on - strand, within AMB_RS23640 at 4451.717 kb on + strand, within AMB_RS23640 at 4451.717 kb on + strand, within AMB_RS23640 at 4451.718 kb on - strand, within AMB_RS23640 at 4451.718 kb on - strand, within AMB_RS23640 at 4451.723 kb on + strand, within AMB_RS23640 at 4451.723 kb on + strand, within AMB_RS23640 at 4451.723 kb on + strand, within AMB_RS23640 at 4451.723 kb on + strand, within AMB_RS23640 at 4451.723 kb on + strand, within AMB_RS23640 at 4451.724 kb on - strand, within AMB_RS23640 at 4451.724 kb on - strand, within AMB_RS23640 at 4451.724 kb on - strand, within AMB_RS23640 at 4451.724 kb on - strand, within AMB_RS23640 at 4451.724 kb on - strand, within AMB_RS23640 at 4451.724 kb on - strand, within AMB_RS23640 at 4451.724 kb on - strand, within AMB_RS23640 at 4451.747 kb on + strand, within AMB_RS23640 at 4451.747 kb on + strand, within AMB_RS23640 at 4451.747 kb on + strand, within AMB_RS23640 at 4451.748 kb on - strand, within AMB_RS23640 at 4451.748 kb on - strand, within AMB_RS23640 at 4451.748 kb on - strand, within AMB_RS23640 at 4451.748 kb on - strand, within AMB_RS23640
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_nonmag_1 remove 4,449,794 + AMB_RS20480 0.15 -0.6 4,449,794 + AMB_RS20480 0.15 -0.1 4,449,794 + AMB_RS20480 0.15 -0.2 4,449,794 + AMB_RS20480 0.15 -4.0 4,449,795 - AMB_RS20480 0.15 -0.3 4,449,795 - AMB_RS20480 0.15 -3.0 4,449,795 - AMB_RS20480 0.15 -0.2 4,449,795 - AMB_RS20480 0.15 -0.6 4,449,857 + AMB_RS20480 0.35 -2.4 4,449,858 - AMB_RS20480 0.36 +1.5 4,449,914 + AMB_RS20480 0.54 -0.6 4,450,475 + AMB_RS20490 0.86 -0.3 4,450,475 + AMB_RS20490 0.86 -0.9 4,450,476 - AMB_RS20490 0.87 +0.1 4,450,559 + -0.6 4,450,559 + +0.1 4,450,559 + -1.3 4,450,648 + -1.3 4,450,648 + +3.4 4,450,648 + -1.1 4,450,648 + +0.1 4,450,648 + -0.2 4,450,648 + +0.2 4,450,648 + +0.7 4,450,648 + +0.4 4,450,649 - AMB_RS20495 0.10 -1.1 4,450,649 - AMB_RS20495 0.10 +0.1 4,450,649 - AMB_RS20495 0.10 -0.9 4,450,649 - AMB_RS20495 0.10 -0.9 4,450,843 + +0.1 4,450,843 + -1.1 4,450,844 - -0.4 4,450,844 - -0.6 4,450,844 - -1.9 4,450,884 + -3.0 4,450,884 + +1.8 4,450,885 - +0.0 4,450,893 - -1.9 4,451,041 + AMB_RS20500 0.88 -2.2 4,451,182 + -3.9 4,451,182 + -3.4 4,451,182 + -1.3 4,451,182 + +0.3 4,451,253 + +0.8 4,451,253 + -0.3 4,451,254 - -1.6 4,451,254 - -1.4 4,451,384 + -1.6 4,451,384 + -2.2 4,451,385 - -2.4 4,451,385 - -0.4 4,451,385 - -0.7 4,451,385 - -0.3 4,451,457 + -1.1 4,451,457 + +0.8 4,451,457 + -0.9 4,451,457 + +0.1 4,451,458 - -2.5 4,451,458 - -0.7 4,451,458 - -1.3 4,451,547 + AMB_RS23640 0.12 -0.3 4,451,547 + AMB_RS23640 0.12 -1.6 4,451,547 + AMB_RS23640 0.12 -1.7 4,451,547 + AMB_RS23640 0.12 +0.3 4,451,548 - AMB_RS23640 0.12 -1.4 4,451,548 - AMB_RS23640 0.12 -0.9 4,451,548 - AMB_RS23640 0.12 -0.6 4,451,548 - AMB_RS23640 0.12 -0.6 4,451,548 - AMB_RS23640 0.12 -0.0 4,451,625 + AMB_RS23640 0.15 -1.4 4,451,625 + AMB_RS23640 0.15 +0.5 4,451,626 - AMB_RS23640 0.15 -1.1 4,451,626 - AMB_RS23640 0.15 -4.6 4,451,626 - AMB_RS23640 0.15 -0.3 4,451,675 + AMB_RS23640 0.16 -0.6 4,451,676 - AMB_RS23640 0.16 -0.7 4,451,676 - AMB_RS23640 0.16 -0.3 4,451,676 - AMB_RS23640 0.16 -2.5 4,451,676 - AMB_RS23640 0.16 -3.9 4,451,717 + AMB_RS23640 0.18 -1.5 4,451,717 + AMB_RS23640 0.18 +0.1 4,451,718 - AMB_RS23640 0.18 -1.3 4,451,718 - AMB_RS23640 0.18 -0.1 4,451,723 + AMB_RS23640 0.18 +0.1 4,451,723 + AMB_RS23640 0.18 -3.0 4,451,723 + AMB_RS23640 0.18 -1.1 4,451,723 + AMB_RS23640 0.18 -1.5 4,451,723 + AMB_RS23640 0.18 +0.7 4,451,724 - AMB_RS23640 0.18 +1.1 4,451,724 - AMB_RS23640 0.18 +0.7 4,451,724 - AMB_RS23640 0.18 -1.9 4,451,724 - AMB_RS23640 0.18 -0.7 4,451,724 - AMB_RS23640 0.18 -0.4 4,451,724 - AMB_RS23640 0.18 -0.3 4,451,724 - AMB_RS23640 0.18 +0.1 4,451,747 + AMB_RS23640 0.19 -0.4 4,451,747 + AMB_RS23640 0.19 -1.7 4,451,747 + AMB_RS23640 0.19 +0.1 4,451,748 - AMB_RS23640 0.19 -3.1 4,451,748 - AMB_RS23640 0.19 +1.7 4,451,748 - AMB_RS23640 0.19 -0.9 4,451,748 - AMB_RS23640 0.19 +0.7
Or see this region's nucleotide sequence