Experiment: 30_an_nonmag_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS14140 and AMB_RS14145 are separated by 250 nucleotides AMB_RS14145 and AMB_RS14150 are separated by 87 nucleotides AMB_RS14150 and AMB_RS14155 overlap by 1 nucleotides AMB_RS14155 and AMB_RS14160 overlap by 4 nucleotides
AMB_RS14140: AMB_RS14140 - hypothetical protein, at 3,003,063 to 3,004,106
_RS14140
AMB_RS14145: AMB_RS14145 - peptide chain release factor 2, at 3,004,357 to 3,005,472
_RS14145
AMB_RS14150: AMB_RS14150 - hypothetical protein, at 3,005,560 to 3,006,054
_RS14150
AMB_RS14155: AMB_RS14155 - hypothetical protein, at 3,006,054 to 3,006,362
_RS14155
AMB_RS14160: AMB_RS14160 - RNA polymerase sigma factor, at 3,006,359 to 3,007,024
_RS14160
Position (kb)
3004
3005
3006 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4
5 at 3003.547 kb on + strand, within AMB_RS14140 at 3004.137 kb on + strand at 3004.137 kb on + strand at 3004.137 kb on + strand at 3004.138 kb on - strand at 3004.138 kb on - strand at 3004.138 kb on - strand at 3004.138 kb on - strand at 3004.139 kb on + strand at 3004.139 kb on + strand at 3004.139 kb on + strand at 3004.140 kb on - strand at 3004.267 kb on + strand at 3005.510 kb on - strand at 3005.510 kb on - strand at 3005.691 kb on - strand, within AMB_RS14150 at 3005.691 kb on - strand, within AMB_RS14150 at 3005.804 kb on + strand, within AMB_RS14150 at 3005.805 kb on - strand, within AMB_RS14150 at 3006.073 kb on + strand at 3006.074 kb on - strand at 3006.074 kb on - strand at 3006.077 kb on - strand at 3006.077 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_nonmag_1 remove 3,003,547 + AMB_RS14140 0.46 -4.3 3,004,137 + -2.1 3,004,137 + -2.4 3,004,137 + +0.6 3,004,138 - +0.9 3,004,138 - +2.2 3,004,138 - -2.0 3,004,138 - -0.8 3,004,139 + -5.2 3,004,139 + -1.3 3,004,139 + -0.4 3,004,140 - +0.2 3,004,267 + -0.6 3,005,510 - +0.3 3,005,510 - -1.3 3,005,691 - AMB_RS14150 0.26 +0.6 3,005,691 - AMB_RS14150 0.26 -0.4 3,005,804 + AMB_RS14150 0.49 -0.8 3,005,805 - AMB_RS14150 0.49 -1.0 3,006,073 + -1.9 3,006,074 - -0.9 3,006,074 - +5.0 3,006,077 - -0.4 3,006,077 - -1.6
Or see this region's nucleotide sequence