Experiment: 30_an_nonmag_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS07180 and AMB_RS07185 are separated by 131 nucleotides AMB_RS07185 and AMB_RS07190 are separated by 75 nucleotides AMB_RS07190 and AMB_RS07195 are separated by 405 nucleotides
AMB_RS07180: AMB_RS07180 - membrane protein, at 1,544,916 to 1,547,816
_RS07180
AMB_RS07185: AMB_RS07185 - HIT family protein, at 1,547,948 to 1,548,349
_RS07185
AMB_RS07190: AMB_RS07190 - SGNH/GDSL hydrolase family protein, at 1,548,425 to 1,549,591
_RS07190
AMB_RS07195: AMB_RS07195 - acetate--CoA ligase, at 1,549,997 to 1,551,577
_RS07195
Position (kb)
1548
1549
1550 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1547.778 kb on + strand at 1547.778 kb on + strand at 1547.778 kb on + strand at 1547.778 kb on + strand at 1547.779 kb on - strand at 1547.779 kb on - strand at 1547.814 kb on + strand at 1547.814 kb on + strand at 1547.815 kb on - strand at 1548.001 kb on + strand, within AMB_RS07185 at 1548.001 kb on + strand, within AMB_RS07185 at 1548.002 kb on - strand, within AMB_RS07185 at 1548.003 kb on + strand, within AMB_RS07185 at 1548.004 kb on - strand, within AMB_RS07185 at 1548.004 kb on - strand, within AMB_RS07185 at 1548.121 kb on + strand, within AMB_RS07185 at 1548.122 kb on - strand, within AMB_RS07185 at 1548.258 kb on + strand, within AMB_RS07185 at 1548.259 kb on - strand, within AMB_RS07185 at 1548.259 kb on - strand, within AMB_RS07185 at 1548.259 kb on - strand, within AMB_RS07185 at 1548.259 kb on - strand, within AMB_RS07185 at 1548.378 kb on + strand at 1548.379 kb on - strand at 1548.379 kb on - strand at 1548.391 kb on - strand at 1548.463 kb on + strand at 1548.464 kb on - strand at 1548.605 kb on + strand, within AMB_RS07190 at 1548.605 kb on + strand, within AMB_RS07190 at 1548.605 kb on + strand, within AMB_RS07190 at 1548.605 kb on + strand, within AMB_RS07190 at 1548.606 kb on - strand, within AMB_RS07190 at 1548.606 kb on - strand, within AMB_RS07190 at 1548.674 kb on + strand, within AMB_RS07190 at 1548.675 kb on - strand, within AMB_RS07190 at 1548.719 kb on + strand, within AMB_RS07190 at 1548.719 kb on + strand, within AMB_RS07190 at 1548.719 kb on + strand, within AMB_RS07190 at 1548.720 kb on - strand, within AMB_RS07190 at 1548.720 kb on - strand, within AMB_RS07190 at 1548.720 kb on - strand, within AMB_RS07190 at 1548.720 kb on - strand, within AMB_RS07190 at 1548.720 kb on - strand, within AMB_RS07190 at 1548.861 kb on - strand, within AMB_RS07190 at 1548.929 kb on + strand, within AMB_RS07190 at 1549.025 kb on + strand, within AMB_RS07190 at 1549.376 kb on + strand, within AMB_RS07190 at 1549.401 kb on - strand, within AMB_RS07190 at 1549.493 kb on + strand at 1549.493 kb on + strand at 1549.493 kb on + strand at 1549.494 kb on - strand at 1549.496 kb on - strand at 1549.512 kb on - strand at 1549.609 kb on + strand at 1549.610 kb on - strand at 1549.610 kb on - strand at 1549.650 kb on + strand at 1549.650 kb on + strand at 1549.650 kb on + strand at 1549.651 kb on - strand at 1549.651 kb on - strand at 1549.651 kb on - strand at 1549.651 kb on - strand at 1549.651 kb on - strand at 1549.651 kb on - strand at 1549.787 kb on + strand at 1549.788 kb on - strand at 1549.788 kb on - strand at 1549.788 kb on - strand at 1550.039 kb on + strand at 1550.039 kb on + strand at 1550.039 kb on + strand at 1550.039 kb on + strand at 1550.040 kb on - strand at 1550.040 kb on - strand at 1550.040 kb on - strand at 1550.040 kb on - strand at 1550.147 kb on + strand at 1550.147 kb on + strand at 1550.148 kb on - strand at 1550.148 kb on - strand at 1550.148 kb on - strand at 1550.186 kb on + strand, within AMB_RS07195 at 1550.186 kb on + strand, within AMB_RS07195 at 1550.187 kb on - strand, within AMB_RS07195 at 1550.279 kb on + strand, within AMB_RS07195 at 1550.280 kb on - strand, within AMB_RS07195 at 1550.280 kb on - strand, within AMB_RS07195
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_nonmag_1 remove 1,547,778 + -1.1 1,547,778 + +0.4 1,547,778 + -0.8 1,547,778 + -1.8 1,547,779 - +0.5 1,547,779 - -1.1 1,547,814 + +0.3 1,547,814 + -0.1 1,547,815 - +0.5 1,548,001 + AMB_RS07185 0.13 -0.8 1,548,001 + AMB_RS07185 0.13 -1.6 1,548,002 - AMB_RS07185 0.13 -1.1 1,548,003 + AMB_RS07185 0.14 -0.5 1,548,004 - AMB_RS07185 0.14 -0.8 1,548,004 - AMB_RS07185 0.14 -0.8 1,548,121 + AMB_RS07185 0.43 -1.5 1,548,122 - AMB_RS07185 0.43 -0.4 1,548,258 + AMB_RS07185 0.77 +1.6 1,548,259 - AMB_RS07185 0.77 -0.8 1,548,259 - AMB_RS07185 0.77 -0.8 1,548,259 - AMB_RS07185 0.77 +0.3 1,548,259 - AMB_RS07185 0.77 -0.5 1,548,378 + -1.1 1,548,379 - -2.8 1,548,379 - -0.1 1,548,391 - -0.6 1,548,463 + -0.5 1,548,464 - -1.6 1,548,605 + AMB_RS07190 0.15 -0.7 1,548,605 + AMB_RS07190 0.15 -0.1 1,548,605 + AMB_RS07190 0.15 +0.0 1,548,605 + AMB_RS07190 0.15 -3.4 1,548,606 - AMB_RS07190 0.16 -1.1 1,548,606 - AMB_RS07190 0.16 +0.1 1,548,674 + AMB_RS07190 0.21 -1.0 1,548,675 - AMB_RS07190 0.21 -1.3 1,548,719 + AMB_RS07190 0.25 -1.1 1,548,719 + AMB_RS07190 0.25 -3.5 1,548,719 + AMB_RS07190 0.25 -0.5 1,548,720 - AMB_RS07190 0.25 -1.0 1,548,720 - AMB_RS07190 0.25 -3.1 1,548,720 - AMB_RS07190 0.25 -1.7 1,548,720 - AMB_RS07190 0.25 -0.2 1,548,720 - AMB_RS07190 0.25 -3.4 1,548,861 - AMB_RS07190 0.37 +0.4 1,548,929 + AMB_RS07190 0.43 -1.3 1,549,025 + AMB_RS07190 0.51 -0.2 1,549,376 + AMB_RS07190 0.81 -0.4 1,549,401 - AMB_RS07190 0.84 +0.5 1,549,493 + -3.7 1,549,493 + -3.7 1,549,493 + +0.4 1,549,494 - -2.8 1,549,496 - -0.9 1,549,512 - +0.1 1,549,609 + -1.7 1,549,610 - -0.5 1,549,610 - -0.5 1,549,650 + -0.8 1,549,650 + -3.4 1,549,650 + -1.5 1,549,651 - -0.3 1,549,651 - -0.5 1,549,651 - +0.0 1,549,651 - +0.2 1,549,651 - -0.1 1,549,651 - -2.0 1,549,787 + -0.9 1,549,788 - -1.1 1,549,788 - +1.6 1,549,788 - -0.5 1,550,039 + -0.5 1,550,039 + -0.2 1,550,039 + -1.7 1,550,039 + -1.1 1,550,040 - -2.5 1,550,040 - -0.4 1,550,040 - -2.5 1,550,040 - -0.1 1,550,147 + -3.1 1,550,147 + +1.5 1,550,148 - -0.0 1,550,148 - -4.9 1,550,148 - -1.1 1,550,186 + AMB_RS07195 0.12 +0.0 1,550,186 + AMB_RS07195 0.12 -1.6 1,550,187 - AMB_RS07195 0.12 -0.8 1,550,279 + AMB_RS07195 0.18 -1.1 1,550,280 - AMB_RS07195 0.18 +0.1 1,550,280 - AMB_RS07195 0.18 -0.3
Or see this region's nucleotide sequence