Experiment: 30_an_nonmag_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01085 and AMB_RS01090 overlap by 4 nucleotides AMB_RS01090 and AMB_RS01095 overlap by 4 nucleotides AMB_RS01095 and AMB_RS01100 are separated by 92 nucleotides
AMB_RS01085: AMB_RS01085 - signal recognition particle-docking protein FtsY, at 241,552 to 242,550
_RS01085
AMB_RS01090: AMB_RS01090 - tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))- methylthiotransferase MtaB, at 242,547 to 243,905
_RS01090
AMB_RS01095: AMB_RS01095 - diaminopimelate epimerase, at 243,902 to 244,756
_RS01095
AMB_RS01100: AMB_RS01100 - potassium/proton antiporter, at 244,849 to 246,642
_RS01100
Position (kb)
242
243
244 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 241.550 kb on - strand at 241.553 kb on + strand at 241.553 kb on + strand at 241.554 kb on - strand at 241.554 kb on - strand at 242.651 kb on - strand at 244.766 kb on - strand at 244.766 kb on - strand at 244.766 kb on - strand at 244.766 kb on - strand at 244.766 kb on - strand at 244.766 kb on - strand at 244.766 kb on - strand at 244.766 kb on - strand at 244.766 kb on - strand at 244.766 kb on - strand at 244.766 kb on - strand at 244.766 kb on - strand at 244.768 kb on - strand at 244.768 kb on - strand at 244.905 kb on + strand at 244.905 kb on + strand at 244.905 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_nonmag_1 remove 241,550 - +0.9 241,553 + +1.9 241,553 + -1.5 241,554 - +1.9 241,554 - -0.5 242,651 - -2.5 244,766 - -1.9 244,766 - -0.5 244,766 - -0.1 244,766 - -0.1 244,766 - +0.2 244,766 - -1.0 244,766 - +0.3 244,766 - -1.8 244,766 - +0.9 244,766 - -0.3 244,766 - -0.3 244,766 - -2.0 244,768 - -0.1 244,768 - -0.8 244,905 + -1.2 244,905 + +0.7 244,905 + -3.8
Or see this region's nucleotide sequence