Experiment: Fusidic 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purA and yjeB are separated by 204 nucleotides yjeB and vacB are separated by 38 nucleotides
b4177: purA - adenylosuccinate synthetase (NCBI), at 4,402,710 to 4,404,008
purA
b4178: yjeB - predicted DNA-binding transcriptional regulator (NCBI), at 4,404,213 to 4,404,638
yjeB
b4179: vacB - putative enzyme (VIMSS), at 4,404,677 to 4,407,118
vacB
Position (kb)
4404
4405 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4403.242 kb on - strand, within purA at 4403.472 kb on - strand, within purA at 4403.566 kb on - strand, within purA at 4403.574 kb on - strand, within purA at 4403.855 kb on - strand, within purA at 4403.965 kb on + strand at 4403.967 kb on - strand at 4404.050 kb on + strand at 4404.050 kb on + strand at 4404.050 kb on + strand at 4404.050 kb on + strand at 4404.070 kb on - strand at 4404.095 kb on - strand at 4404.095 kb on - strand at 4404.097 kb on + strand at 4404.097 kb on + strand at 4404.169 kb on - strand at 4404.254 kb on + strand at 4404.311 kb on + strand, within yjeB at 4404.311 kb on + strand, within yjeB at 4404.312 kb on + strand, within yjeB at 4404.312 kb on + strand, within yjeB at 4404.364 kb on - strand, within yjeB at 4404.364 kb on - strand, within yjeB at 4404.379 kb on - strand, within yjeB at 4404.379 kb on - strand, within yjeB at 4404.490 kb on - strand, within yjeB at 4404.536 kb on + strand, within yjeB at 4404.536 kb on + strand, within yjeB at 4404.536 kb on + strand, within yjeB at 4404.536 kb on + strand, within yjeB at 4404.536 kb on + strand, within yjeB at 4404.544 kb on - strand, within yjeB at 4404.544 kb on - strand, within yjeB at 4404.567 kb on + strand, within yjeB at 4404.583 kb on - strand, within yjeB at 4404.600 kb on - strand at 4404.620 kb on - strand at 4404.695 kb on + strand at 4404.695 kb on + strand at 4404.806 kb on - strand at 4404.819 kb on - strand at 4404.873 kb on - strand at 4404.898 kb on + strand at 4404.898 kb on + strand at 4404.898 kb on + strand at 4404.928 kb on + strand, within vacB at 4404.928 kb on + strand, within vacB at 4404.952 kb on + strand, within vacB at 4404.966 kb on + strand, within vacB at 4404.966 kb on + strand, within vacB at 4405.013 kb on - strand, within vacB at 4405.066 kb on - strand, within vacB at 4405.066 kb on - strand, within vacB at 4405.080 kb on - strand, within vacB at 4405.151 kb on - strand, within vacB at 4405.187 kb on + strand, within vacB at 4405.245 kb on + strand, within vacB at 4405.278 kb on - strand, within vacB at 4405.313 kb on + strand, within vacB at 4405.348 kb on + strand, within vacB at 4405.358 kb on - strand, within vacB at 4405.463 kb on - strand, within vacB at 4405.463 kb on - strand, within vacB at 4405.580 kb on + strand, within vacB at 4405.590 kb on + strand, within vacB at 4405.590 kb on + strand, within vacB at 4405.590 kb on + strand, within vacB
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.1 mg/ml remove 4,403,242 - purA b4177 0.41 -2.3 4,403,472 - purA b4177 0.59 -1.1 4,403,566 - purA b4177 0.66 +0.0 4,403,574 - purA b4177 0.67 +0.0 4,403,855 - purA b4177 0.88 +0.8 4,403,965 + +0.1 4,403,967 - -1.0 4,404,050 + +0.5 4,404,050 + +1.2 4,404,050 + -0.2 4,404,050 + +1.8 4,404,070 - -0.5 4,404,095 - +0.5 4,404,095 - +0.5 4,404,097 + -1.0 4,404,097 + +0.3 4,404,169 - -0.7 4,404,254 + -0.4 4,404,311 + yjeB b4178 0.23 -0.4 4,404,311 + yjeB b4178 0.23 -1.0 4,404,312 + yjeB b4178 0.23 +0.0 4,404,312 + yjeB b4178 0.23 -1.3 4,404,364 - yjeB b4178 0.35 +1.0 4,404,364 - yjeB b4178 0.35 -2.0 4,404,379 - yjeB b4178 0.39 -0.2 4,404,379 - yjeB b4178 0.39 -0.6 4,404,490 - yjeB b4178 0.65 +0.3 4,404,536 + yjeB b4178 0.76 -0.6 4,404,536 + yjeB b4178 0.76 +1.0 4,404,536 + yjeB b4178 0.76 -0.4 4,404,536 + yjeB b4178 0.76 -1.6 4,404,536 + yjeB b4178 0.76 +1.0 4,404,544 - yjeB b4178 0.78 +0.8 4,404,544 - yjeB b4178 0.78 +0.4 4,404,567 + yjeB b4178 0.83 -0.8 4,404,583 - yjeB b4178 0.87 -0.9 4,404,600 - +0.8 4,404,620 - +2.0 4,404,695 + -1.1 4,404,695 + +0.2 4,404,806 - +0.0 4,404,819 - +0.0 4,404,873 - -1.1 4,404,898 + +2.0 4,404,898 + +1.4 4,404,898 + +0.4 4,404,928 + vacB b4179 0.10 -0.6 4,404,928 + vacB b4179 0.10 +0.0 4,404,952 + vacB b4179 0.11 +3.3 4,404,966 + vacB b4179 0.12 -1.1 4,404,966 + vacB b4179 0.12 -1.8 4,405,013 - vacB b4179 0.14 -0.3 4,405,066 - vacB b4179 0.16 +1.0 4,405,066 - vacB b4179 0.16 -1.6 4,405,080 - vacB b4179 0.17 +1.0 4,405,151 - vacB b4179 0.19 +0.0 4,405,187 + vacB b4179 0.21 +0.0 4,405,245 + vacB b4179 0.23 -0.8 4,405,278 - vacB b4179 0.25 -2.6 4,405,313 + vacB b4179 0.26 +0.4 4,405,348 + vacB b4179 0.27 -0.3 4,405,358 - vacB b4179 0.28 -1.5 4,405,463 - vacB b4179 0.32 -0.3 4,405,463 - vacB b4179 0.32 -1.8 4,405,580 + vacB b4179 0.37 -1.0 4,405,590 + vacB b4179 0.37 +0.0 4,405,590 + vacB b4179 0.37 +1.0 4,405,590 + vacB b4179 0.37 +1.0
Or see this region's nucleotide sequence