Experiment: Fusidic 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aaeA and yhcR are separated by 7 nucleotides yhcR and aaeR are separated by 182 nucleotides aaeR and tldD are separated by 133 nucleotides
b3241: aaeA - p-hydroxybenzoic acid efflux system component (NCBI), at 3,386,216 to 3,387,148
aaeA
b3242: yhcR - orf, hypothetical protein (VIMSS), at 3,387,156 to 3,387,359
yhcR
b3243: aaeR - predicted DNA-binding transcriptional regulator, efflux system (NCBI), at 3,387,542 to 3,388,471
aaeR
b3244: tldD - predicted peptidase (NCBI), at 3,388,605 to 3,390,050
tldD
Position (kb)
3387
3388
3389 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3386.569 kb on + strand, within aaeA at 3386.633 kb on + strand, within aaeA at 3386.634 kb on + strand, within aaeA at 3386.637 kb on - strand, within aaeA at 3386.642 kb on - strand, within aaeA at 3386.642 kb on - strand, within aaeA at 3386.655 kb on + strand, within aaeA at 3386.675 kb on + strand, within aaeA at 3386.763 kb on + strand, within aaeA at 3386.763 kb on + strand, within aaeA at 3386.803 kb on - strand, within aaeA at 3386.803 kb on - strand, within aaeA at 3386.803 kb on - strand, within aaeA at 3386.803 kb on - strand, within aaeA at 3386.805 kb on - strand, within aaeA at 3386.858 kb on + strand, within aaeA at 3386.882 kb on - strand, within aaeA at 3386.918 kb on + strand, within aaeA at 3386.958 kb on - strand, within aaeA at 3387.015 kb on + strand, within aaeA at 3387.015 kb on + strand, within aaeA at 3387.023 kb on - strand, within aaeA at 3387.045 kb on - strand, within aaeA at 3387.051 kb on + strand, within aaeA at 3387.051 kb on + strand, within aaeA at 3387.107 kb on + strand at 3387.200 kb on - strand, within yhcR at 3387.200 kb on - strand, within yhcR at 3387.213 kb on - strand, within yhcR at 3387.213 kb on - strand, within yhcR at 3387.213 kb on - strand, within yhcR at 3387.215 kb on - strand, within yhcR at 3387.237 kb on + strand, within yhcR at 3387.237 kb on + strand, within yhcR at 3387.242 kb on - strand, within yhcR at 3387.242 kb on - strand, within yhcR at 3387.245 kb on + strand, within yhcR at 3387.264 kb on + strand, within yhcR at 3387.264 kb on + strand, within yhcR at 3387.334 kb on - strand, within yhcR at 3387.365 kb on - strand at 3387.370 kb on + strand at 3387.372 kb on + strand at 3387.390 kb on - strand at 3387.431 kb on - strand at 3387.493 kb on - strand at 3387.495 kb on - strand at 3387.505 kb on - strand at 3387.571 kb on + strand at 3387.571 kb on + strand at 3387.614 kb on - strand at 3387.705 kb on + strand, within aaeR at 3387.775 kb on - strand, within aaeR at 3387.784 kb on - strand, within aaeR at 3387.784 kb on - strand, within aaeR at 3387.811 kb on + strand, within aaeR at 3387.827 kb on - strand, within aaeR at 3387.843 kb on - strand, within aaeR at 3387.843 kb on - strand, within aaeR at 3388.115 kb on - strand, within aaeR at 3388.115 kb on - strand, within aaeR at 3388.423 kb on + strand at 3388.478 kb on - strand at 3388.483 kb on + strand at 3388.610 kb on - strand at 3388.702 kb on + strand at 3388.702 kb on + strand at 3388.750 kb on - strand, within tldD at 3388.765 kb on + strand, within tldD at 3388.765 kb on + strand, within tldD at 3388.858 kb on + strand, within tldD at 3388.866 kb on - strand, within tldD at 3388.896 kb on + strand, within tldD at 3388.946 kb on + strand, within tldD at 3388.946 kb on + strand, within tldD at 3388.946 kb on + strand, within tldD at 3388.946 kb on + strand, within tldD at 3388.948 kb on + strand, within tldD at 3388.948 kb on + strand, within tldD at 3388.954 kb on - strand, within tldD at 3388.954 kb on - strand, within tldD at 3388.962 kb on - strand, within tldD at 3388.962 kb on - strand, within tldD at 3388.998 kb on - strand, within tldD at 3389.049 kb on - strand, within tldD at 3389.049 kb on - strand, within tldD at 3389.051 kb on + strand, within tldD at 3389.090 kb on - strand, within tldD at 3389.109 kb on - strand, within tldD at 3389.167 kb on + strand, within tldD at 3389.167 kb on + strand, within tldD at 3389.172 kb on - strand, within tldD at 3389.174 kb on + strand, within tldD at 3389.174 kb on + strand, within tldD at 3389.174 kb on + strand, within tldD at 3389.182 kb on - strand, within tldD at 3389.182 kb on - strand, within tldD at 3389.242 kb on + strand, within tldD at 3389.332 kb on + strand, within tldD at 3389.332 kb on + strand, within tldD at 3389.350 kb on + strand, within tldD
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.1 mg/ml remove 3,386,569 + aaeA b3241 0.38 +0.1 3,386,633 + aaeA b3241 0.45 +0.3 3,386,634 + aaeA b3241 0.45 -1.1 3,386,637 - aaeA b3241 0.45 -1.9 3,386,642 - aaeA b3241 0.46 -0.6 3,386,642 - aaeA b3241 0.46 +0.0 3,386,655 + aaeA b3241 0.47 +0.2 3,386,675 + aaeA b3241 0.49 +0.1 3,386,763 + aaeA b3241 0.59 +0.2 3,386,763 + aaeA b3241 0.59 +0.4 3,386,803 - aaeA b3241 0.63 -0.3 3,386,803 - aaeA b3241 0.63 +0.6 3,386,803 - aaeA b3241 0.63 +2.0 3,386,803 - aaeA b3241 0.63 +1.3 3,386,805 - aaeA b3241 0.63 +0.3 3,386,858 + aaeA b3241 0.69 +0.8 3,386,882 - aaeA b3241 0.71 -0.4 3,386,918 + aaeA b3241 0.75 -0.0 3,386,958 - aaeA b3241 0.80 +0.1 3,387,015 + aaeA b3241 0.86 -1.3 3,387,015 + aaeA b3241 0.86 -1.3 3,387,023 - aaeA b3241 0.86 -0.3 3,387,045 - aaeA b3241 0.89 +0.2 3,387,051 + aaeA b3241 0.89 +0.0 3,387,051 + aaeA b3241 0.89 -1.3 3,387,107 + -0.3 3,387,200 - yhcR b3242 0.22 +0.4 3,387,200 - yhcR b3242 0.22 +0.3 3,387,213 - yhcR b3242 0.28 -1.5 3,387,213 - yhcR b3242 0.28 +0.0 3,387,213 - yhcR b3242 0.28 +0.2 3,387,215 - yhcR b3242 0.29 -2.8 3,387,237 + yhcR b3242 0.40 -1.3 3,387,237 + yhcR b3242 0.40 +2.0 3,387,242 - yhcR b3242 0.42 -0.6 3,387,242 - yhcR b3242 0.42 -0.3 3,387,245 + yhcR b3242 0.44 -0.6 3,387,264 + yhcR b3242 0.53 -0.2 3,387,264 + yhcR b3242 0.53 -1.3 3,387,334 - yhcR b3242 0.87 -1.2 3,387,365 - -1.3 3,387,370 + +0.2 3,387,372 + +0.0 3,387,390 - -1.0 3,387,431 - -0.3 3,387,493 - -1.3 3,387,495 - +0.1 3,387,505 - -0.6 3,387,571 + +1.6 3,387,571 + +0.5 3,387,614 - -0.8 3,387,705 + aaeR b3243 0.18 -0.4 3,387,775 - aaeR b3243 0.25 -0.4 3,387,784 - aaeR b3243 0.26 -1.3 3,387,784 - aaeR b3243 0.26 +0.3 3,387,811 + aaeR b3243 0.29 -0.5 3,387,827 - aaeR b3243 0.31 +0.3 3,387,843 - aaeR b3243 0.32 -0.4 3,387,843 - aaeR b3243 0.32 -1.3 3,388,115 - aaeR b3243 0.62 +1.0 3,388,115 - aaeR b3243 0.62 -1.8 3,388,423 + +0.3 3,388,478 - +0.6 3,388,483 + +0.1 3,388,610 - +0.8 3,388,702 + -0.4 3,388,702 + -0.2 3,388,750 - tldD b3244 0.10 +0.2 3,388,765 + tldD b3244 0.11 +1.0 3,388,765 + tldD b3244 0.11 +0.4 3,388,858 + tldD b3244 0.17 +0.0 3,388,866 - tldD b3244 0.18 -0.1 3,388,896 + tldD b3244 0.20 +0.2 3,388,946 + tldD b3244 0.24 +2.0 3,388,946 + tldD b3244 0.24 +0.0 3,388,946 + tldD b3244 0.24 -0.3 3,388,946 + tldD b3244 0.24 -0.2 3,388,948 + tldD b3244 0.24 +1.0 3,388,948 + tldD b3244 0.24 -0.6 3,388,954 - tldD b3244 0.24 -0.2 3,388,954 - tldD b3244 0.24 +0.4 3,388,962 - tldD b3244 0.25 -0.2 3,388,962 - tldD b3244 0.25 +1.0 3,388,998 - tldD b3244 0.27 +0.2 3,389,049 - tldD b3244 0.31 -0.3 3,389,049 - tldD b3244 0.31 -0.8 3,389,051 + tldD b3244 0.31 -1.6 3,389,090 - tldD b3244 0.34 +0.8 3,389,109 - tldD b3244 0.35 -0.4 3,389,167 + tldD b3244 0.39 -0.2 3,389,167 + tldD b3244 0.39 -0.7 3,389,172 - tldD b3244 0.39 +0.3 3,389,174 + tldD b3244 0.39 -1.4 3,389,174 + tldD b3244 0.39 +0.3 3,389,174 + tldD b3244 0.39 +0.5 3,389,182 - tldD b3244 0.40 -0.4 3,389,182 - tldD b3244 0.40 -0.7 3,389,242 + tldD b3244 0.44 -0.4 3,389,332 + tldD b3244 0.50 +0.0 3,389,332 + tldD b3244 0.50 +0.6 3,389,350 + tldD b3244 0.52 +2.0
Or see this region's nucleotide sequence