Strain Fitness in Escherichia coli BW25113 around b1413

Experiment: Fusidic 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntynbD and azoR are separated by 53 nucleotidesazoR and hrpA are separated by 200 nucleotideshrpA and ydcF are separated by 271 nucleotides b1411: ynbD - predicted phosphatase, inner membrane protein (NCBI), at 1,478,933 to 1,480,225 ynbD b1412: azoR - acyl carrier protein phosphodiesterase (NCBI), at 1,480,279 to 1,480,884 azoR b1413: hrpA - ATP-dependent helicase (RefSeq), at 1,481,085 to 1,484,987 hrpA b1414: ydcF - hypothetical protein (NCBI), at 1,485,259 to 1,486,059 ydcF Position (kb) 1481 1482 1483 1484 1485Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1480.154 kb on - strandat 1480.167 kb on + strandat 1480.271 kb on + strandat 1480.271 kb on + strandat 1480.667 kb on - strand, within azoRat 1480.729 kb on + strand, within azoRat 1480.729 kb on + strand, within azoRat 1481.461 kb on + strandat 1481.461 kb on + strandat 1481.608 kb on + strand, within hrpAat 1481.635 kb on - strand, within hrpAat 1481.646 kb on + strand, within hrpAat 1481.646 kb on + strand, within hrpAat 1481.804 kb on + strand, within hrpAat 1481.804 kb on + strand, within hrpAat 1481.916 kb on + strand, within hrpAat 1481.956 kb on + strand, within hrpAat 1481.956 kb on + strand, within hrpAat 1482.025 kb on + strand, within hrpAat 1482.062 kb on + strand, within hrpAat 1482.181 kb on + strand, within hrpAat 1482.187 kb on - strand, within hrpAat 1482.187 kb on - strand, within hrpAat 1482.187 kb on - strand, within hrpAat 1482.187 kb on - strand, within hrpAat 1482.314 kb on + strand, within hrpAat 1482.314 kb on + strand, within hrpAat 1482.417 kb on - strand, within hrpAat 1482.553 kb on + strand, within hrpAat 1483.054 kb on + strand, within hrpAat 1483.877 kb on + strand, within hrpAat 1483.921 kb on + strand, within hrpAat 1484.433 kb on + strand, within hrpAat 1484.566 kb on - strand, within hrpAat 1484.615 kb on - strandat 1484.883 kb on + strandat 1484.883 kb on + strandat 1485.446 kb on + strand, within ydcFat 1485.502 kb on + strand, within ydcFat 1485.553 kb on - strand, within ydcFat 1485.833 kb on + strand, within ydcFat 1485.869 kb on - strand, within ydcFat 1485.881 kb on + strand, within ydcFat 1485.881 kb on + strand, within ydcFat 1485.887 kb on - strand, within ydcFat 1485.899 kb on - strand, within ydcFat 1485.899 kb on - strand, within ydcF

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Per-strain Table

Position Strand Gene LocusTag Fraction Fusidic 0.1 mg/ml
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1,480,154 - +0.9
1,480,167 + +2.7
1,480,271 + -0.3
1,480,271 + +0.8
1,480,667 - azoR b1412 0.64 +1.0
1,480,729 + azoR b1412 0.74 +2.8
1,480,729 + azoR b1412 0.74 +3.1
1,481,461 + -1.2
1,481,461 + +0.2
1,481,608 + hrpA b1413 0.13 -0.2
1,481,635 - hrpA b1413 0.14 +0.3
1,481,646 + hrpA b1413 0.14 -0.6
1,481,646 + hrpA b1413 0.14 -1.1
1,481,804 + hrpA b1413 0.18 -0.7
1,481,804 + hrpA b1413 0.18 -0.4
1,481,916 + hrpA b1413 0.21 +1.8
1,481,956 + hrpA b1413 0.22 -1.5
1,481,956 + hrpA b1413 0.22 -1.3
1,482,025 + hrpA b1413 0.24 -1.2
1,482,062 + hrpA b1413 0.25 -2.1
1,482,181 + hrpA b1413 0.28 -0.7
1,482,187 - hrpA b1413 0.28 +0.1
1,482,187 - hrpA b1413 0.28 -0.4
1,482,187 - hrpA b1413 0.28 -0.2
1,482,187 - hrpA b1413 0.28 +0.2
1,482,314 + hrpA b1413 0.31 -0.0
1,482,314 + hrpA b1413 0.31 -2.0
1,482,417 - hrpA b1413 0.34 -0.0
1,482,553 + hrpA b1413 0.38 -1.2
1,483,054 + hrpA b1413 0.50 -0.6
1,483,877 + hrpA b1413 0.72 -0.1
1,483,921 + hrpA b1413 0.73 -2.2
1,484,433 + hrpA b1413 0.86 +0.3
1,484,566 - hrpA b1413 0.89 -1.1
1,484,615 - +0.3
1,484,883 + -0.9
1,484,883 + -1.6
1,485,446 + ydcF b1414 0.23 +0.4
1,485,502 + ydcF b1414 0.30 +0.8
1,485,553 - ydcF b1414 0.37 +1.1
1,485,833 + ydcF b1414 0.72 -0.0
1,485,869 - ydcF b1414 0.76 -2.0
1,485,881 + ydcF b1414 0.78 +0.3
1,485,881 + ydcF b1414 0.78 +0.8
1,485,887 - ydcF b1414 0.78 -0.3
1,485,899 - ydcF b1414 0.80 +0.2
1,485,899 - ydcF b1414 0.80 -0.1

Or see this region's nucleotide sequence