Experiment: Fusidic 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt flgA and flgB are separated by 154 nucleotides flgB and flgC are separated by 3 nucleotides flgC and flgD are separated by 11 nucleotides flgD and flgE are separated by 24 nucleotides
b1072: flgA - flagellar basal body P-ring biosynthesis protein A (NCBI), at 1,129,427 to 1,130,086
flgA
b1073: flgB - flagellar basal-body rod protein B (NCBI), at 1,130,241 to 1,130,657
flgB
b1074: flgC - flagellar basal-body rod protein C (NCBI), at 1,130,661 to 1,131,065
flgC
b1075: flgD - flagellar basal body rod modification protein D (NCBI), at 1,131,077 to 1,131,772
flgD
b1076: flgE - flagellar hook protein E (NCBI), at 1,131,797 to 1,133,005
flgE
Position (kb)
1130
1131
1132 Strain fitness (log2 ratio)
-1
0
1 at 1129.663 kb on + strand, within flgA at 1129.716 kb on + strand, within flgA at 1129.771 kb on + strand, within flgA at 1129.771 kb on + strand, within flgA at 1129.810 kb on + strand, within flgA at 1129.811 kb on - strand, within flgA at 1129.813 kb on + strand, within flgA at 1129.815 kb on + strand, within flgA at 1129.815 kb on + strand, within flgA at 1129.958 kb on + strand, within flgA at 1130.045 kb on + strand at 1130.097 kb on + strand at 1130.110 kb on + strand at 1130.161 kb on + strand at 1130.236 kb on - strand at 1130.238 kb on - strand at 1130.238 kb on - strand at 1130.270 kb on - strand at 1130.352 kb on - strand, within flgB at 1130.352 kb on - strand, within flgB at 1130.411 kb on + strand, within flgB at 1130.411 kb on + strand, within flgB at 1130.456 kb on - strand, within flgB at 1130.456 kb on - strand, within flgB at 1130.513 kb on - strand, within flgB at 1130.570 kb on - strand, within flgB at 1130.693 kb on - strand at 1130.701 kb on + strand at 1131.014 kb on + strand, within flgC at 1131.014 kb on + strand, within flgC at 1131.054 kb on - strand at 1131.111 kb on + strand at 1131.325 kb on + strand, within flgD at 1131.362 kb on - strand, within flgD at 1131.362 kb on - strand, within flgD at 1131.586 kb on + strand, within flgD at 1131.594 kb on - strand, within flgD at 1131.794 kb on + strand at 1131.794 kb on + strand at 1131.846 kb on - strand at 1131.996 kb on - strand, within flgE at 1131.996 kb on - strand, within flgE at 1132.002 kb on - strand, within flgE at 1132.010 kb on + strand, within flgE
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.1 mg/ml remove 1,129,663 + flgA b1072 0.36 -0.3 1,129,716 + flgA b1072 0.44 -0.3 1,129,771 + flgA b1072 0.52 -0.1 1,129,771 + flgA b1072 0.52 -0.9 1,129,810 + flgA b1072 0.58 +0.1 1,129,811 - flgA b1072 0.58 +0.3 1,129,813 + flgA b1072 0.58 +1.0 1,129,815 + flgA b1072 0.59 +1.5 1,129,815 + flgA b1072 0.59 -0.3 1,129,958 + flgA b1072 0.80 -0.9 1,130,045 + -0.1 1,130,097 + -0.4 1,130,110 + +0.1 1,130,161 + +0.1 1,130,236 - +1.4 1,130,238 - +0.7 1,130,238 - -0.9 1,130,270 - -0.4 1,130,352 - flgB b1073 0.27 -0.9 1,130,352 - flgB b1073 0.27 -0.4 1,130,411 + flgB b1073 0.41 +0.2 1,130,411 + flgB b1073 0.41 -0.3 1,130,456 - flgB b1073 0.52 +1.2 1,130,456 - flgB b1073 0.52 +0.4 1,130,513 - flgB b1073 0.65 +0.1 1,130,570 - flgB b1073 0.79 +0.4 1,130,693 - +0.7 1,130,701 + +0.7 1,131,014 + flgC b1074 0.87 -0.6 1,131,014 + flgC b1074 0.87 -0.9 1,131,054 - -0.3 1,131,111 + +0.7 1,131,325 + flgD b1075 0.36 +0.7 1,131,362 - flgD b1075 0.41 -1.3 1,131,362 - flgD b1075 0.41 -0.0 1,131,586 + flgD b1075 0.73 -0.9 1,131,594 - flgD b1075 0.74 +0.1 1,131,794 + +1.1 1,131,794 + -1.3 1,131,846 - +1.1 1,131,996 - flgE b1076 0.16 -0.3 1,131,996 - flgE b1076 0.16 +0.2 1,132,002 - flgE b1076 0.17 -0.5 1,132,010 + flgE b1076 0.18 +0.5
Or see this region's nucleotide sequence