Experiment: Fusidic 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybiJ and ybiI are separated by 264 nucleotides ybiI and ybiX are separated by 73 nucleotides ybiX and fiu are separated by 41 nucleotides
b0802: ybiJ - hypothetical protein (NCBI), at 836,888 to 837,148
ybiJ
b0803: ybiI - hypothetical protein (NCBI), at 837,413 to 837,679
ybiI
b0804: ybiX - putative enzyme (VIMSS), at 837,753 to 838,430
ybiX
b0805: fiu - predicted iron outer membrane transporter (NCBI), at 838,472 to 840,754
fiu
Position (kb) 837
838
839 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 836.768 kb on - strand at 836.787 kb on - strand at 836.938 kb on + strand, within ybiJ at 837.079 kb on - strand, within ybiJ at 837.104 kb on + strand, within ybiJ at 837.196 kb on - strand at 837.320 kb on + strand at 837.320 kb on + strand at 837.320 kb on + strand at 837.320 kb on + strand at 837.389 kb on + strand at 837.406 kb on + strand at 837.438 kb on - strand at 837.438 kb on - strand at 837.446 kb on + strand, within ybiI at 837.550 kb on - strand, within ybiI at 837.567 kb on - strand, within ybiI at 837.576 kb on - strand, within ybiI at 837.630 kb on + strand, within ybiI at 837.638 kb on - strand, within ybiI at 837.723 kb on + strand at 837.800 kb on + strand at 837.853 kb on - strand, within ybiX at 838.096 kb on + strand, within ybiX at 838.096 kb on + strand, within ybiX at 838.140 kb on - strand, within ybiX at 838.140 kb on - strand, within ybiX at 838.195 kb on - strand, within ybiX at 838.195 kb on - strand, within ybiX at 838.195 kb on - strand, within ybiX at 838.253 kb on - strand, within ybiX at 838.511 kb on - strand at 838.511 kb on - strand at 838.519 kb on + strand at 838.563 kb on + strand at 838.596 kb on - strand at 838.790 kb on - strand, within fiu at 838.790 kb on - strand, within fiu at 838.790 kb on - strand, within fiu at 838.797 kb on + strand, within fiu at 838.800 kb on - strand, within fiu at 838.800 kb on - strand, within fiu at 838.983 kb on + strand, within fiu at 839.091 kb on + strand, within fiu at 839.110 kb on + strand, within fiu at 839.230 kb on + strand, within fiu
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.1 mg/ml remove 836,768 - -0.6 836,787 - -0.2 836,938 + ybiJ b0802 0.19 -0.3 837,079 - ybiJ b0802 0.73 -0.2 837,104 + ybiJ b0802 0.83 -0.2 837,196 - +1.3 837,320 + -0.4 837,320 + +0.8 837,320 + -0.3 837,320 + +0.4 837,389 + +0.4 837,406 + +0.8 837,438 - -0.2 837,438 - +0.9 837,446 + ybiI b0803 0.12 +0.4 837,550 - ybiI b0803 0.51 +0.7 837,567 - ybiI b0803 0.58 -0.4 837,576 - ybiI b0803 0.61 -0.4 837,630 + ybiI b0803 0.81 -1.2 837,638 - ybiI b0803 0.84 +0.8 837,723 + -0.2 837,800 + -0.0 837,853 - ybiX b0804 0.15 +0.4 838,096 + ybiX b0804 0.51 -0.2 838,096 + ybiX b0804 0.51 -0.9 838,140 - ybiX b0804 0.57 -1.2 838,140 - ybiX b0804 0.57 -1.6 838,195 - ybiX b0804 0.65 -0.3 838,195 - ybiX b0804 0.65 -2.9 838,195 - ybiX b0804 0.65 +3.1 838,253 - ybiX b0804 0.74 +0.1 838,511 - -0.7 838,511 - -0.5 838,519 + +0.7 838,563 + -0.5 838,596 - +0.1 838,790 - fiu b0805 0.14 +0.4 838,790 - fiu b0805 0.14 -0.8 838,790 - fiu b0805 0.14 -0.5 838,797 + fiu b0805 0.14 -0.2 838,800 - fiu b0805 0.14 -0.4 838,800 - fiu b0805 0.14 -0.4 838,983 + fiu b0805 0.22 +0.7 839,091 + fiu b0805 0.27 +0.4 839,110 + fiu b0805 0.28 -0.6 839,230 + fiu b0805 0.33 -0.5
Or see this region's nucleotide sequence