Experiment: Fusidic 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt prpR and prpB are separated by 238 nucleotides prpB and prpC are separated by 439 nucleotides
b0330: prpR - DNA-binding transcriptional activator (NCBI), at 346,081 to 347,667
prpR
b0331: prpB - 2-methylisocitrate lyase (NCBI), at 347,906 to 348,796
prpB
b0333: prpC - 2-methylcitrate synthase (NCBI), at 349,236 to 350,405
prpC
Position (kb) 347
348
349 Strain fitness (log2 ratio) -2
-1
0
1
2 at 346.945 kb on - strand, within prpR at 347.006 kb on - strand, within prpR at 347.006 kb on - strand, within prpR at 347.098 kb on - strand, within prpR at 347.190 kb on - strand, within prpR at 347.190 kb on - strand, within prpR at 347.321 kb on - strand, within prpR at 347.328 kb on + strand, within prpR at 347.346 kb on + strand, within prpR at 347.346 kb on + strand, within prpR at 347.391 kb on - strand, within prpR at 347.399 kb on - strand, within prpR at 347.650 kb on - strand at 347.650 kb on - strand at 347.896 kb on - strand at 347.896 kb on - strand at 348.109 kb on + strand, within prpB at 348.217 kb on + strand, within prpB at 348.294 kb on - strand, within prpB at 348.493 kb on - strand, within prpB at 348.511 kb on + strand, within prpB at 348.554 kb on + strand, within prpB at 348.653 kb on + strand, within prpB at 348.655 kb on + strand, within prpB at 348.655 kb on + strand, within prpB at 348.702 kb on + strand, within prpB at 348.702 kb on + strand, within prpB at 348.774 kb on - strand at 348.858 kb on + strand at 348.858 kb on + strand at 348.883 kb on - strand at 348.932 kb on + strand at 348.932 kb on + strand at 348.953 kb on + strand at 348.958 kb on + strand at 349.167 kb on + strand at 349.167 kb on + strand at 349.232 kb on + strand at 349.328 kb on + strand at 349.336 kb on - strand at 349.427 kb on + strand, within prpC at 349.502 kb on + strand, within prpC at 349.502 kb on + strand, within prpC at 349.642 kb on - strand, within prpC at 349.691 kb on + strand, within prpC at 349.691 kb on + strand, within prpC at 349.744 kb on - strand, within prpC
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.1 mg/ml remove 346,945 - prpR b0330 0.54 +0.1 347,006 - prpR b0330 0.58 +0.5 347,006 - prpR b0330 0.58 -0.2 347,098 - prpR b0330 0.64 +0.5 347,190 - prpR b0330 0.70 +0.1 347,190 - prpR b0330 0.70 +1.8 347,321 - prpR b0330 0.78 +0.3 347,328 + prpR b0330 0.79 +1.2 347,346 + prpR b0330 0.80 +1.2 347,346 + prpR b0330 0.80 -0.1 347,391 - prpR b0330 0.83 +1.4 347,399 - prpR b0330 0.83 +0.2 347,650 - +1.0 347,650 - +0.7 347,896 - +0.8 347,896 - -0.8 348,109 + prpB b0331 0.23 -0.2 348,217 + prpB b0331 0.35 -0.6 348,294 - prpB b0331 0.44 -1.8 348,493 - prpB b0331 0.66 -0.1 348,511 + prpB b0331 0.68 +0.2 348,554 + prpB b0331 0.73 -0.2 348,653 + prpB b0331 0.84 -1.1 348,655 + prpB b0331 0.84 +0.7 348,655 + prpB b0331 0.84 -0.9 348,702 + prpB b0331 0.89 +0.8 348,702 + prpB b0331 0.89 -1.2 348,774 - +0.2 348,858 + -0.2 348,858 + +1.0 348,883 - +2.1 348,932 + -0.5 348,932 + +0.8 348,953 + +0.4 348,958 + -0.7 349,167 + -0.2 349,167 + -0.2 349,232 + +0.4 349,328 + -0.8 349,336 - +0.1 349,427 + prpC b0333 0.16 -0.8 349,502 + prpC b0333 0.23 -1.3 349,502 + prpC b0333 0.23 +0.1 349,642 - prpC b0333 0.35 +1.1 349,691 + prpC b0333 0.39 +0.4 349,691 + prpC b0333 0.39 +0.2 349,744 - prpC b0333 0.43 -0.4
Or see this region's nucleotide sequence