Experiment: 30_an_mag_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS13060 and AMB_RS13065 are separated by 3 nucleotides AMB_RS13065 and AMB_RS13070 are separated by 2 nucleotides AMB_RS13070 and AMB_RS13075 are separated by 108 nucleotides
AMB_RS13060: AMB_RS13060 - acetyl-CoA C-acyltransferase, at 2,781,078 to 2,782,274
_RS13060
AMB_RS13065: AMB_RS13065 - 3-hydroxyacyl-CoA dehydrogenase, at 2,782,278 to 2,783,798
_RS13065
AMB_RS13070: AMB_RS13070 - enoyl-CoA hydratase, at 2,783,801 to 2,784,571
_RS13070
AMB_RS13075: AMB_RS13075 - IclR family transcriptional regulator, at 2,784,680 to 2,785,516
_RS13075
Position (kb)
2782
2783
2784 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2781.676 kb on + strand, within AMB_RS13060 at 2781.676 kb on + strand, within AMB_RS13060 at 2781.677 kb on - strand, within AMB_RS13060 at 2781.677 kb on - strand, within AMB_RS13060 at 2781.704 kb on - strand, within AMB_RS13060 at 2781.943 kb on + strand, within AMB_RS13060 at 2781.943 kb on + strand, within AMB_RS13060 at 2781.944 kb on - strand, within AMB_RS13060 at 2781.944 kb on - strand, within AMB_RS13060 at 2782.255 kb on + strand at 2782.255 kb on + strand at 2782.255 kb on + strand at 2782.255 kb on + strand at 2782.255 kb on + strand at 2782.256 kb on - strand at 2782.256 kb on - strand at 2782.256 kb on - strand at 2782.259 kb on + strand at 2782.259 kb on + strand at 2782.260 kb on - strand at 2782.260 kb on - strand at 2782.260 kb on - strand at 2782.261 kb on + strand at 2782.261 kb on + strand at 2782.262 kb on - strand at 2782.262 kb on - strand at 2782.262 kb on - strand at 2782.262 kb on - strand at 2782.262 kb on - strand at 2782.262 kb on - strand at 2782.309 kb on - strand at 2782.658 kb on + strand, within AMB_RS13065 at 2782.659 kb on - strand, within AMB_RS13065 at 2782.659 kb on - strand, within AMB_RS13065 at 2782.933 kb on + strand, within AMB_RS13065 at 2782.934 kb on - strand, within AMB_RS13065 at 2782.939 kb on + strand, within AMB_RS13065 at 2782.939 kb on + strand, within AMB_RS13065 at 2782.940 kb on - strand, within AMB_RS13065 at 2782.940 kb on - strand, within AMB_RS13065 at 2782.940 kb on - strand, within AMB_RS13065 at 2782.940 kb on - strand, within AMB_RS13065 at 2782.940 kb on - strand, within AMB_RS13065 at 2782.940 kb on - strand, within AMB_RS13065 at 2783.027 kb on - strand, within AMB_RS13065 at 2783.029 kb on + strand, within AMB_RS13065 at 2783.029 kb on + strand, within AMB_RS13065 at 2783.030 kb on - strand, within AMB_RS13065 at 2783.030 kb on - strand, within AMB_RS13065 at 2783.030 kb on - strand, within AMB_RS13065 at 2783.030 kb on - strand, within AMB_RS13065 at 2783.030 kb on - strand, within AMB_RS13065 at 2783.038 kb on + strand, within AMB_RS13065 at 2783.038 kb on + strand, within AMB_RS13065 at 2783.039 kb on - strand, within AMB_RS13065 at 2783.039 kb on - strand, within AMB_RS13065 at 2783.429 kb on + strand, within AMB_RS13065 at 2783.429 kb on + strand, within AMB_RS13065 at 2783.430 kb on - strand, within AMB_RS13065 at 2783.430 kb on - strand, within AMB_RS13065 at 2783.430 kb on - strand, within AMB_RS13065 at 2783.430 kb on - strand, within AMB_RS13065 at 2783.430 kb on - strand, within AMB_RS13065 at 2783.814 kb on + strand at 2783.814 kb on + strand at 2783.814 kb on + strand at 2783.814 kb on + strand at 2783.815 kb on - strand at 2783.815 kb on - strand at 2784.264 kb on + strand, within AMB_RS13070 at 2784.264 kb on + strand, within AMB_RS13070 at 2784.630 kb on + strand at 2784.630 kb on + strand at 2784.630 kb on + strand at 2784.631 kb on - strand at 2784.631 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_mag_3 remove 2,781,676 + AMB_RS13060 0.50 +0.6 2,781,676 + AMB_RS13060 0.50 +0.1 2,781,677 - AMB_RS13060 0.50 -0.7 2,781,677 - AMB_RS13060 0.50 -1.5 2,781,704 - AMB_RS13060 0.52 +0.3 2,781,943 + AMB_RS13060 0.72 -0.1 2,781,943 + AMB_RS13060 0.72 -0.5 2,781,944 - AMB_RS13060 0.72 -0.3 2,781,944 - AMB_RS13060 0.72 -1.7 2,782,255 + -0.9 2,782,255 + -0.9 2,782,255 + -2.4 2,782,255 + -1.3 2,782,255 + -2.4 2,782,256 - -1.0 2,782,256 - -0.5 2,782,256 - -0.4 2,782,259 + -0.7 2,782,259 + +0.1 2,782,260 - -1.9 2,782,260 - -3.3 2,782,260 - -0.2 2,782,261 + -1.5 2,782,261 + -0.4 2,782,262 - -0.1 2,782,262 - -1.4 2,782,262 - +0.9 2,782,262 - +0.1 2,782,262 - +0.3 2,782,262 - -0.0 2,782,309 - +0.1 2,782,658 + AMB_RS13065 0.25 -1.7 2,782,659 - AMB_RS13065 0.25 +0.2 2,782,659 - AMB_RS13065 0.25 +0.5 2,782,933 + AMB_RS13065 0.43 +0.5 2,782,934 - AMB_RS13065 0.43 -1.9 2,782,939 + AMB_RS13065 0.43 -1.0 2,782,939 + AMB_RS13065 0.43 -0.3 2,782,940 - AMB_RS13065 0.44 +0.1 2,782,940 - AMB_RS13065 0.44 -1.0 2,782,940 - AMB_RS13065 0.44 -2.4 2,782,940 - AMB_RS13065 0.44 -1.0 2,782,940 - AMB_RS13065 0.44 -0.7 2,782,940 - AMB_RS13065 0.44 +0.3 2,783,027 - AMB_RS13065 0.49 -2.6 2,783,029 + AMB_RS13065 0.49 -0.3 2,783,029 + AMB_RS13065 0.49 -0.7 2,783,030 - AMB_RS13065 0.49 -1.5 2,783,030 - AMB_RS13065 0.49 +0.3 2,783,030 - AMB_RS13065 0.49 -0.3 2,783,030 - AMB_RS13065 0.49 -0.3 2,783,030 - AMB_RS13065 0.49 -0.1 2,783,038 + AMB_RS13065 0.50 -0.3 2,783,038 + AMB_RS13065 0.50 -0.3 2,783,039 - AMB_RS13065 0.50 +0.7 2,783,039 - AMB_RS13065 0.50 +0.3 2,783,429 + AMB_RS13065 0.76 +0.1 2,783,429 + AMB_RS13065 0.76 +1.3 2,783,430 - AMB_RS13065 0.76 -3.0 2,783,430 - AMB_RS13065 0.76 +0.2 2,783,430 - AMB_RS13065 0.76 +0.3 2,783,430 - AMB_RS13065 0.76 -3.3 2,783,430 - AMB_RS13065 0.76 +0.8 2,783,814 + -1.9 2,783,814 + -0.4 2,783,814 + -0.7 2,783,814 + +0.4 2,783,815 - -0.7 2,783,815 - -1.7 2,784,264 + AMB_RS13070 0.60 -0.3 2,784,264 + AMB_RS13070 0.60 -1.2 2,784,630 + -0.5 2,784,630 + +0.2 2,784,630 + -1.7 2,784,631 - -0.3 2,784,631 - +0.3
Or see this region's nucleotide sequence