Experiment: 30_an_mag_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS12955 and AMB_RS12960 are separated by 65 nucleotides AMB_RS12960 and AMB_RS12965 are separated by 151 nucleotides AMB_RS12965 and AMB_RS12970 are separated by 39 nucleotides AMB_RS12970 and AMB_RS12975 are separated by 209 nucleotides
AMB_RS12955: AMB_RS12955 - DNA-3-methyladenine glycosylase I, at 2,759,273 to 2,759,827
_RS12955
AMB_RS12960: AMB_RS12960 - methylmalonyl-CoA epimerase, at 2,759,893 to 2,760,297
_RS12960
AMB_RS12965: AMB_RS12965 - penicillin acylase family protein, at 2,760,449 to 2,762,860
_RS12965
AMB_RS12970: AMB_RS12970 - flavin reductase, at 2,762,900 to 2,763,403
_RS12970
AMB_RS12975: AMB_RS12975 - flagellar motor stator protein MotA, at 2,763,613 to 2,764,473
_RS12975
Position (kb)
2760
2761
2762
2763 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2759.614 kb on - strand, within AMB_RS12955 at 2759.763 kb on + strand, within AMB_RS12955 at 2759.818 kb on - strand at 2760.053 kb on + strand, within AMB_RS12960 at 2760.054 kb on - strand, within AMB_RS12960 at 2760.054 kb on - strand, within AMB_RS12960 at 2760.288 kb on + strand at 2760.288 kb on + strand at 2760.288 kb on + strand at 2760.288 kb on + strand at 2760.328 kb on + strand at 2760.328 kb on + strand at 2760.328 kb on + strand at 2760.329 kb on - strand at 2760.329 kb on - strand at 2760.329 kb on - strand at 2760.329 kb on - strand at 2760.329 kb on - strand at 2760.339 kb on + strand at 2760.339 kb on + strand at 2760.339 kb on + strand at 2760.339 kb on + strand at 2760.339 kb on + strand at 2760.340 kb on - strand at 2760.340 kb on - strand at 2760.340 kb on - strand at 2760.340 kb on - strand at 2760.341 kb on + strand at 2760.342 kb on - strand at 2760.342 kb on - strand at 2760.342 kb on - strand at 2760.342 kb on - strand at 2760.405 kb on + strand at 2760.734 kb on + strand, within AMB_RS12965 at 2760.735 kb on - strand, within AMB_RS12965 at 2760.735 kb on - strand, within AMB_RS12965 at 2760.735 kb on - strand, within AMB_RS12965 at 2760.735 kb on - strand, within AMB_RS12965 at 2760.854 kb on + strand, within AMB_RS12965 at 2760.854 kb on + strand, within AMB_RS12965 at 2760.855 kb on - strand, within AMB_RS12965 at 2760.855 kb on - strand, within AMB_RS12965 at 2760.875 kb on + strand, within AMB_RS12965 at 2760.875 kb on + strand, within AMB_RS12965 at 2760.876 kb on - strand, within AMB_RS12965 at 2760.876 kb on - strand, within AMB_RS12965 at 2760.939 kb on - strand, within AMB_RS12965 at 2760.968 kb on + strand, within AMB_RS12965 at 2760.969 kb on - strand, within AMB_RS12965 at 2760.969 kb on - strand, within AMB_RS12965 at 2760.973 kb on + strand, within AMB_RS12965 at 2760.973 kb on + strand, within AMB_RS12965 at 2761.346 kb on + strand, within AMB_RS12965 at 2761.347 kb on - strand, within AMB_RS12965 at 2761.347 kb on - strand, within AMB_RS12965 at 2761.347 kb on - strand, within AMB_RS12965 at 2762.006 kb on + strand, within AMB_RS12965 at 2762.006 kb on + strand, within AMB_RS12965 at 2762.006 kb on + strand, within AMB_RS12965 at 2762.006 kb on + strand, within AMB_RS12965 at 2762.006 kb on + strand, within AMB_RS12965 at 2762.007 kb on - strand, within AMB_RS12965 at 2762.627 kb on + strand at 2762.628 kb on - strand at 2762.628 kb on - strand at 2762.765 kb on + strand at 2762.804 kb on + strand at 2762.817 kb on + strand at 2762.817 kb on + strand at 2762.940 kb on + strand at 2762.940 kb on + strand at 2762.940 kb on + strand at 2762.941 kb on - strand at 2762.941 kb on - strand at 2762.941 kb on - strand at 2762.941 kb on - strand at 2762.955 kb on + strand, within AMB_RS12970 at 2762.956 kb on - strand, within AMB_RS12970 at 2762.956 kb on - strand, within AMB_RS12970 at 2762.956 kb on - strand, within AMB_RS12970 at 2762.956 kb on - strand, within AMB_RS12970 at 2763.155 kb on - strand, within AMB_RS12970 at 2763.155 kb on - strand, within AMB_RS12970 at 2763.155 kb on - strand, within AMB_RS12970 at 2763.435 kb on - strand at 2763.441 kb on - strand at 2763.441 kb on - strand at 2763.441 kb on - strand at 2763.546 kb on + strand at 2763.548 kb on + strand at 2763.664 kb on + strand at 2763.665 kb on - strand at 2763.835 kb on + strand, within AMB_RS12975 at 2763.835 kb on + strand, within AMB_RS12975 at 2763.836 kb on - strand, within AMB_RS12975 at 2763.860 kb on - strand, within AMB_RS12975 at 2763.860 kb on - strand, within AMB_RS12975
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_mag_3 remove 2,759,614 - AMB_RS12955 0.61 +0.3 2,759,763 + AMB_RS12955 0.88 +0.2 2,759,818 - -1.1 2,760,053 + AMB_RS12960 0.40 +1.6 2,760,054 - AMB_RS12960 0.40 +0.6 2,760,054 - AMB_RS12960 0.40 -0.7 2,760,288 + +2.2 2,760,288 + +1.3 2,760,288 + +1.7 2,760,288 + -0.3 2,760,328 + +0.6 2,760,328 + +0.3 2,760,328 + -1.3 2,760,329 - +0.4 2,760,329 - +0.0 2,760,329 - -0.0 2,760,329 - -0.7 2,760,329 - -1.8 2,760,339 + -0.7 2,760,339 + -1.1 2,760,339 + -3.1 2,760,339 + -1.7 2,760,339 + -0.3 2,760,340 - +0.3 2,760,340 - +0.2 2,760,340 - +0.9 2,760,340 - +0.4 2,760,341 + -0.3 2,760,342 - -0.4 2,760,342 - +0.8 2,760,342 - -3.5 2,760,342 - -0.3 2,760,405 + +0.4 2,760,734 + AMB_RS12965 0.12 -1.7 2,760,735 - AMB_RS12965 0.12 -0.3 2,760,735 - AMB_RS12965 0.12 -1.7 2,760,735 - AMB_RS12965 0.12 +0.3 2,760,735 - AMB_RS12965 0.12 +0.4 2,760,854 + AMB_RS12965 0.17 -0.6 2,760,854 + AMB_RS12965 0.17 +0.7 2,760,855 - AMB_RS12965 0.17 -1.3 2,760,855 - AMB_RS12965 0.17 +1.4 2,760,875 + AMB_RS12965 0.18 +0.2 2,760,875 + AMB_RS12965 0.18 +0.0 2,760,876 - AMB_RS12965 0.18 -0.2 2,760,876 - AMB_RS12965 0.18 +1.3 2,760,939 - AMB_RS12965 0.20 +0.2 2,760,968 + AMB_RS12965 0.22 -0.1 2,760,969 - AMB_RS12965 0.22 -1.1 2,760,969 - AMB_RS12965 0.22 -0.4 2,760,973 + AMB_RS12965 0.22 +0.4 2,760,973 + AMB_RS12965 0.22 -0.3 2,761,346 + AMB_RS12965 0.37 -0.4 2,761,347 - AMB_RS12965 0.37 -1.3 2,761,347 - AMB_RS12965 0.37 -0.3 2,761,347 - AMB_RS12965 0.37 +0.1 2,762,006 + AMB_RS12965 0.65 +0.0 2,762,006 + AMB_RS12965 0.65 -2.1 2,762,006 + AMB_RS12965 0.65 +0.2 2,762,006 + AMB_RS12965 0.65 -1.1 2,762,006 + AMB_RS12965 0.65 -2.3 2,762,007 - AMB_RS12965 0.65 -0.7 2,762,627 + +0.3 2,762,628 - +0.7 2,762,628 - -0.4 2,762,765 + +0.0 2,762,804 + +0.7 2,762,817 + +0.7 2,762,817 + -1.2 2,762,940 + -0.1 2,762,940 + -0.7 2,762,940 + +0.6 2,762,941 - -1.1 2,762,941 - +0.2 2,762,941 - +0.1 2,762,941 - -1.3 2,762,955 + AMB_RS12970 0.11 -0.1 2,762,956 - AMB_RS12970 0.11 -0.7 2,762,956 - AMB_RS12970 0.11 +0.1 2,762,956 - AMB_RS12970 0.11 -1.7 2,762,956 - AMB_RS12970 0.11 -1.3 2,763,155 - AMB_RS12970 0.51 -0.5 2,763,155 - AMB_RS12970 0.51 +0.2 2,763,155 - AMB_RS12970 0.51 -1.7 2,763,435 - +0.3 2,763,441 - +0.5 2,763,441 - +0.4 2,763,441 - -0.5 2,763,546 + -0.1 2,763,548 + -0.1 2,763,664 + -2.2 2,763,665 - -1.7 2,763,835 + AMB_RS12975 0.26 +0.2 2,763,835 + AMB_RS12975 0.26 -0.1 2,763,836 - AMB_RS12975 0.26 -0.7 2,763,860 - AMB_RS12975 0.29 +0.3 2,763,860 - AMB_RS12975 0.29 -0.3
Or see this region's nucleotide sequence