Experiment: 30_an_mag_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS09080 and AMB_RS09085 are separated by 90 nucleotides AMB_RS09085 and AMB_RS09090 are separated by 155 nucleotides AMB_RS09090 and AMB_RS09095 overlap by 4 nucleotides AMB_RS09095 and AMB_RS09100 are separated by 5 nucleotides
AMB_RS09080: AMB_RS09080 - peptide-modifying radical SAM enzyme CbpB, at 1,933,803 to 1,935,182
_RS09080
AMB_RS09085: AMB_RS09085 - DUF4189 domain-containing protein, at 1,935,273 to 1,935,632
_RS09085
AMB_RS09090: AMB_RS09090 - hypothetical protein, at 1,935,788 to 1,936,960
_RS09090
AMB_RS09095: AMB_RS09095 - hypothetical protein, at 1,936,957 to 1,937,424
_RS09095
AMB_RS09100: AMB_RS09100 - hypothetical protein, at 1,937,430 to 1,938,800
_RS09100
Position (kb)
1935
1936
1937 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1935.057 kb on + strand at 1935.057 kb on + strand at 1935.057 kb on + strand at 1935.058 kb on - strand at 1935.058 kb on - strand at 1935.069 kb on + strand at 1935.070 kb on - strand at 1935.070 kb on - strand at 1935.070 kb on - strand at 1935.087 kb on + strand at 1935.087 kb on + strand at 1935.088 kb on - strand at 1935.126 kb on + strand at 1935.126 kb on + strand at 1935.126 kb on + strand at 1935.126 kb on + strand at 1935.126 kb on + strand at 1935.126 kb on + strand at 1935.127 kb on - strand at 1935.127 kb on - strand at 1935.127 kb on - strand at 1935.127 kb on - strand at 1935.127 kb on - strand at 1935.127 kb on - strand at 1935.127 kb on - strand at 1935.272 kb on + strand at 1935.272 kb on + strand at 1935.272 kb on + strand at 1935.272 kb on + strand at 1935.272 kb on + strand at 1935.272 kb on + strand at 1935.273 kb on - strand at 1935.273 kb on - strand at 1935.273 kb on - strand at 1935.273 kb on - strand at 1935.273 kb on - strand at 1935.273 kb on - strand at 1935.397 kb on - strand, within AMB_RS09085 at 1935.514 kb on - strand, within AMB_RS09085 at 1935.514 kb on - strand, within AMB_RS09085 at 1935.531 kb on + strand, within AMB_RS09085 at 1935.531 kb on + strand, within AMB_RS09085 at 1935.532 kb on - strand, within AMB_RS09085 at 1935.532 kb on - strand, within AMB_RS09085 at 1935.630 kb on + strand at 1935.630 kb on + strand at 1935.631 kb on - strand at 1935.634 kb on - strand at 1935.634 kb on - strand at 1935.641 kb on + strand at 1935.641 kb on + strand at 1935.641 kb on + strand at 1935.641 kb on + strand at 1935.641 kb on + strand at 1935.642 kb on - strand at 1935.642 kb on - strand at 1935.778 kb on + strand at 1935.779 kb on - strand at 1935.779 kb on - strand at 1936.126 kb on - strand, within AMB_RS09090 at 1936.146 kb on + strand, within AMB_RS09090 at 1936.146 kb on + strand, within AMB_RS09090 at 1936.146 kb on + strand, within AMB_RS09090 at 1936.146 kb on + strand, within AMB_RS09090 at 1936.146 kb on + strand, within AMB_RS09090 at 1936.147 kb on - strand, within AMB_RS09090 at 1936.147 kb on - strand, within AMB_RS09090 at 1936.147 kb on - strand, within AMB_RS09090 at 1936.674 kb on + strand, within AMB_RS09090 at 1936.747 kb on - strand, within AMB_RS09090 at 1936.747 kb on - strand, within AMB_RS09090 at 1936.869 kb on + strand at 1936.985 kb on + strand at 1937.024 kb on + strand, within AMB_RS09095 at 1937.102 kb on + strand, within AMB_RS09095 at 1937.103 kb on - strand, within AMB_RS09095 at 1937.171 kb on - strand, within AMB_RS09095 at 1937.213 kb on + strand, within AMB_RS09095 at 1937.213 kb on + strand, within AMB_RS09095 at 1937.214 kb on - strand, within AMB_RS09095 at 1937.944 kb on + strand, within AMB_RS09100 at 1937.945 kb on - strand, within AMB_RS09100
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_mag_3 remove 1,935,057 + -2.9 1,935,057 + +0.7 1,935,057 + +0.4 1,935,058 - -0.6 1,935,058 - +0.2 1,935,069 + -0.7 1,935,070 - -0.9 1,935,070 - -2.7 1,935,070 - -0.3 1,935,087 + +0.7 1,935,087 + -2.4 1,935,088 - +0.1 1,935,126 + +1.3 1,935,126 + -0.4 1,935,126 + +0.3 1,935,126 + +0.0 1,935,126 + -1.1 1,935,126 + -2.4 1,935,127 - +0.3 1,935,127 - -0.4 1,935,127 - -2.2 1,935,127 - -2.1 1,935,127 - -0.7 1,935,127 - +0.4 1,935,127 - -1.3 1,935,272 + -0.7 1,935,272 + -2.1 1,935,272 + +0.2 1,935,272 + +1.3 1,935,272 + -1.6 1,935,272 + -0.3 1,935,273 - -0.2 1,935,273 - -1.1 1,935,273 - -0.1 1,935,273 - -0.3 1,935,273 - -1.6 1,935,273 - -2.4 1,935,397 - AMB_RS09085 0.34 +0.3 1,935,514 - AMB_RS09085 0.67 -2.3 1,935,514 - AMB_RS09085 0.67 -0.2 1,935,531 + AMB_RS09085 0.72 +0.8 1,935,531 + AMB_RS09085 0.72 +0.0 1,935,532 - AMB_RS09085 0.72 +0.3 1,935,532 - AMB_RS09085 0.72 -0.1 1,935,630 + +0.8 1,935,630 + -0.6 1,935,631 - +0.5 1,935,634 - +0.4 1,935,634 - -2.3 1,935,641 + +1.4 1,935,641 + +0.3 1,935,641 + +0.2 1,935,641 + -0.7 1,935,641 + +1.3 1,935,642 - -0.6 1,935,642 - -1.3 1,935,778 + +0.9 1,935,779 - -1.7 1,935,779 - -1.6 1,936,126 - AMB_RS09090 0.29 +0.0 1,936,146 + AMB_RS09090 0.31 +0.2 1,936,146 + AMB_RS09090 0.31 -0.4 1,936,146 + AMB_RS09090 0.31 -2.8 1,936,146 + AMB_RS09090 0.31 -1.9 1,936,146 + AMB_RS09090 0.31 +0.3 1,936,147 - AMB_RS09090 0.31 -3.0 1,936,147 - AMB_RS09090 0.31 -0.7 1,936,147 - AMB_RS09090 0.31 -0.1 1,936,674 + AMB_RS09090 0.76 -0.0 1,936,747 - AMB_RS09090 0.82 -1.1 1,936,747 - AMB_RS09090 0.82 -3.7 1,936,869 + -0.1 1,936,985 + +0.6 1,937,024 + AMB_RS09095 0.14 +0.3 1,937,102 + AMB_RS09095 0.31 -2.6 1,937,103 - AMB_RS09095 0.31 -1.6 1,937,171 - AMB_RS09095 0.46 -2.3 1,937,213 + AMB_RS09095 0.55 -2.4 1,937,213 + AMB_RS09095 0.55 +0.7 1,937,214 - AMB_RS09095 0.55 -1.9 1,937,944 + AMB_RS09100 0.37 +0.5 1,937,945 - AMB_RS09100 0.38 +0.4
Or see this region's nucleotide sequence