Experiment: 30_an_mag_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS06905 and AMB_RS06910 overlap by 4 nucleotides AMB_RS06910 and AMB_RS06915 are separated by 5 nucleotides AMB_RS06915 and AMB_RS06920 are separated by 26 nucleotides
AMB_RS06905: AMB_RS06905 - NAD-dependent dehydratase, at 1,479,613 to 1,480,605
_RS06905
AMB_RS06910: AMB_RS06910 - glycosyl transferase, at 1,480,602 to 1,481,765
_RS06910
AMB_RS06915: AMB_RS06915 - squalene synthase HpnD, at 1,481,771 to 1,482,631
_RS06915
AMB_RS06920: AMB_RS06920 - squalene--hopene cyclase, at 1,482,658 to 1,484,612
_RS06920
Position (kb)
1480
1481
1482 Strain fitness (log2 ratio)
-2
-1
0
1 at 1481.241 kb on + strand, within AMB_RS06910 at 1481.397 kb on + strand, within AMB_RS06910 at 1481.442 kb on + strand, within AMB_RS06910 at 1481.589 kb on + strand, within AMB_RS06910 at 1481.694 kb on + strand at 1481.694 kb on + strand at 1481.694 kb on + strand at 1481.694 kb on + strand at 1481.694 kb on + strand at 1481.694 kb on + strand at 1481.694 kb on + strand at 1481.694 kb on + strand at 1481.697 kb on + strand at 1482.629 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_mag_3 remove 1,481,241 + AMB_RS06910 0.55 -0.7 1,481,397 + AMB_RS06910 0.68 -1.2 1,481,442 + AMB_RS06910 0.72 +0.3 1,481,589 + AMB_RS06910 0.85 +1.3 1,481,694 + -0.4 1,481,694 + -0.2 1,481,694 + -1.0 1,481,694 + +0.2 1,481,694 + -2.6 1,481,694 + -2.2 1,481,694 + -2.0 1,481,694 + -0.2 1,481,697 + -1.5 1,482,629 + -1.0
Or see this region's nucleotide sequence