Experiment: 30_an_mag_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS05445 and AMB_RS23200 are separated by 16 nucleotides AMB_RS23200 and AMB_RS05455 are separated by 180 nucleotides AMB_RS05455 and AMB_RS05460 overlap by 49 nucleotides AMB_RS05460 and AMB_RS05465 overlap by 80 nucleotides
AMB_RS05445: AMB_RS05445 - MBOAT family protein, at 1,128,101 to 1,129,525
_RS05445
AMB_RS23200: AMB_RS23200 - NUDIX hydrolase, at 1,129,542 to 1,130,246
_RS23200
AMB_RS05455: AMB_RS05455 - glycosyltransferase family 2 protein, at 1,130,427 to 1,131,452
_RS05455
AMB_RS05460: AMB_RS05460 - UDP-N-acetylmuramyl pentapeptide phosphotransferase, at 1,131,404 to 1,132,267
_RS05460
AMB_RS05465: AMB_RS05465 - nucleoside-diphosphate sugar epimerase, at 1,132,188 to 1,133,195
_RS05465
Position (kb)
1130
1131
1132 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1129.442 kb on - strand at 1129.739 kb on - strand, within AMB_RS23200 at 1129.799 kb on + strand, within AMB_RS23200 at 1130.089 kb on + strand, within AMB_RS23200 at 1130.225 kb on + strand at 1130.226 kb on - strand at 1130.246 kb on + strand at 1130.246 kb on + strand at 1130.260 kb on + strand at 1130.260 kb on + strand at 1130.260 kb on + strand at 1130.261 kb on - strand at 1130.262 kb on + strand at 1130.262 kb on + strand at 1130.262 kb on + strand at 1130.262 kb on + strand at 1130.263 kb on - strand at 1130.285 kb on + strand at 1130.286 kb on - strand at 1130.286 kb on - strand at 1130.286 kb on - strand at 1130.316 kb on + strand at 1130.316 kb on + strand at 1130.413 kb on + strand at 1130.459 kb on + strand at 1130.459 kb on + strand at 1130.459 kb on + strand at 1130.460 kb on - strand at 1130.496 kb on + strand at 1130.496 kb on + strand at 1130.497 kb on - strand at 1130.547 kb on + strand, within AMB_RS05455 at 1130.547 kb on + strand, within AMB_RS05455 at 1130.548 kb on - strand, within AMB_RS05455 at 1130.548 kb on - strand, within AMB_RS05455 at 1130.634 kb on - strand, within AMB_RS05455 at 1130.879 kb on + strand, within AMB_RS05455 at 1130.947 kb on + strand, within AMB_RS05455 at 1130.983 kb on - strand, within AMB_RS05455 at 1130.983 kb on - strand, within AMB_RS05455 at 1131.081 kb on + strand, within AMB_RS05455 at 1131.081 kb on + strand, within AMB_RS05455 at 1131.081 kb on + strand, within AMB_RS05455 at 1131.082 kb on - strand, within AMB_RS05455 at 1131.082 kb on - strand, within AMB_RS05455 at 1131.139 kb on + strand, within AMB_RS05455 at 1131.139 kb on + strand, within AMB_RS05455 at 1131.140 kb on - strand, within AMB_RS05455 at 1131.140 kb on - strand, within AMB_RS05455 at 1131.140 kb on - strand, within AMB_RS05455 at 1131.143 kb on + strand, within AMB_RS05455 at 1131.177 kb on + strand, within AMB_RS05455 at 1131.197 kb on - strand, within AMB_RS05455 at 1131.198 kb on + strand, within AMB_RS05455 at 1131.199 kb on - strand, within AMB_RS05455 at 1131.204 kb on + strand, within AMB_RS05455 at 1131.205 kb on - strand, within AMB_RS05455 at 1131.231 kb on - strand, within AMB_RS05455 at 1131.267 kb on + strand, within AMB_RS05455 at 1131.271 kb on - strand, within AMB_RS05455 at 1131.278 kb on + strand, within AMB_RS05455 at 1131.383 kb on + strand at 1131.383 kb on + strand at 1131.384 kb on - strand at 1131.406 kb on - strand at 1131.450 kb on + strand at 1131.521 kb on + strand, within AMB_RS05460 at 1131.637 kb on + strand, within AMB_RS05460 at 1131.638 kb on - strand, within AMB_RS05460 at 1132.189 kb on + strand at 1132.189 kb on + strand at 1132.190 kb on - strand at 1132.304 kb on + strand, within AMB_RS05465 at 1132.305 kb on - strand, within AMB_RS05465 at 1132.344 kb on - strand, within AMB_RS05465 at 1132.347 kb on + strand, within AMB_RS05465 at 1132.347 kb on + strand, within AMB_RS05465 at 1132.348 kb on - strand, within AMB_RS05465 at 1132.348 kb on - strand, within AMB_RS05465 at 1132.348 kb on - strand, within AMB_RS05465 at 1132.348 kb on - strand, within AMB_RS05465 at 1132.385 kb on + strand, within AMB_RS05465
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_mag_3 remove 1,129,442 - -0.6 1,129,739 - AMB_RS23200 0.28 +0.0 1,129,799 + AMB_RS23200 0.36 +0.3 1,130,089 + AMB_RS23200 0.78 -0.1 1,130,225 + -0.6 1,130,226 - +0.4 1,130,246 + -0.1 1,130,246 + -1.2 1,130,260 + +0.4 1,130,260 + -1.8 1,130,260 + +0.1 1,130,261 - -0.2 1,130,262 + -0.2 1,130,262 + -0.2 1,130,262 + -0.4 1,130,262 + -2.3 1,130,263 - -1.8 1,130,285 + -3.2 1,130,286 - +0.2 1,130,286 - +0.9 1,130,286 - -0.6 1,130,316 + -2.9 1,130,316 + +0.4 1,130,413 + -0.7 1,130,459 + -1.2 1,130,459 + +0.2 1,130,459 + -1.2 1,130,460 - -1.0 1,130,496 + -0.1 1,130,496 + -0.1 1,130,497 - -0.8 1,130,547 + AMB_RS05455 0.12 -0.0 1,130,547 + AMB_RS05455 0.12 +0.7 1,130,548 - AMB_RS05455 0.12 -1.9 1,130,548 - AMB_RS05455 0.12 -2.7 1,130,634 - AMB_RS05455 0.20 -1.0 1,130,879 + AMB_RS05455 0.44 -2.0 1,130,947 + AMB_RS05455 0.51 -0.6 1,130,983 - AMB_RS05455 0.54 -0.6 1,130,983 - AMB_RS05455 0.54 -0.2 1,131,081 + AMB_RS05455 0.64 -1.2 1,131,081 + AMB_RS05455 0.64 -0.5 1,131,081 + AMB_RS05455 0.64 -0.8 1,131,082 - AMB_RS05455 0.64 +0.0 1,131,082 - AMB_RS05455 0.64 +0.3 1,131,139 + AMB_RS05455 0.69 -1.6 1,131,139 + AMB_RS05455 0.69 -0.2 1,131,140 - AMB_RS05455 0.69 -0.2 1,131,140 - AMB_RS05455 0.69 -2.7 1,131,140 - AMB_RS05455 0.69 -0.9 1,131,143 + AMB_RS05455 0.70 +0.0 1,131,177 + AMB_RS05455 0.73 -2.2 1,131,197 - AMB_RS05455 0.75 -1.0 1,131,198 + AMB_RS05455 0.75 -0.1 1,131,199 - AMB_RS05455 0.75 -1.1 1,131,204 + AMB_RS05455 0.76 +0.2 1,131,205 - AMB_RS05455 0.76 +0.3 1,131,231 - AMB_RS05455 0.78 -2.0 1,131,267 + AMB_RS05455 0.82 -1.0 1,131,271 - AMB_RS05455 0.82 +0.3 1,131,278 + AMB_RS05455 0.83 +0.2 1,131,383 + +0.2 1,131,383 + -0.9 1,131,384 - -0.8 1,131,406 - -1.0 1,131,450 + +0.8 1,131,521 + AMB_RS05460 0.14 -2.4 1,131,637 + AMB_RS05460 0.27 -0.1 1,131,638 - AMB_RS05460 0.27 +0.2 1,132,189 + -0.2 1,132,189 + -0.0 1,132,190 - -1.2 1,132,304 + AMB_RS05465 0.12 -2.7 1,132,305 - AMB_RS05465 0.12 -1.6 1,132,344 - AMB_RS05465 0.15 +0.0 1,132,347 + AMB_RS05465 0.16 -2.2 1,132,347 + AMB_RS05465 0.16 -0.2 1,132,348 - AMB_RS05465 0.16 +0.5 1,132,348 - AMB_RS05465 0.16 -0.6 1,132,348 - AMB_RS05465 0.16 +0.1 1,132,348 - AMB_RS05465 0.16 +0.4 1,132,385 + AMB_RS05465 0.20 +0.5
Or see this region's nucleotide sequence