Experiment: 30_an_mag_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01175 and AMB_RS01180 overlap by 16 nucleotides AMB_RS01180 and AMB_RS01185 are separated by 3 nucleotides AMB_RS01185 and AMB_RS01190 are separated by 192 nucleotides
AMB_RS01175: AMB_RS01175 - C4-dicarboxylate ABC transporter, at 260,935 to 262,242
_RS01175
AMB_RS01180: AMB_RS01180 - dihydrofolate reductase, at 262,227 to 262,748
_RS01180
AMB_RS01185: AMB_RS01185 - HD domain-containing protein, at 262,752 to 264,527
_RS01185
AMB_RS01190: AMB_RS01190 - UbiD family decarboxylase, at 264,720 to 266,234
_RS01190
Position (kb) 262
263
264
265 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 261.871 kb on + strand, within AMB_RS01175 at 261.871 kb on + strand, within AMB_RS01175 at 261.871 kb on + strand, within AMB_RS01175 at 261.871 kb on + strand, within AMB_RS01175 at 261.871 kb on + strand, within AMB_RS01175 at 261.871 kb on + strand, within AMB_RS01175 at 261.872 kb on - strand, within AMB_RS01175 at 261.872 kb on - strand, within AMB_RS01175 at 261.872 kb on - strand, within AMB_RS01175 at 261.935 kb on - strand, within AMB_RS01175 at 261.973 kb on + strand, within AMB_RS01175 at 261.973 kb on + strand, within AMB_RS01175 at 261.974 kb on - strand, within AMB_RS01175 at 262.012 kb on - strand, within AMB_RS01175 at 262.181 kb on - strand at 262.181 kb on - strand at 262.240 kb on + strand at 262.241 kb on - strand at 262.241 kb on - strand at 262.241 kb on - strand at 262.241 kb on - strand at 262.241 kb on - strand at 262.265 kb on + strand at 262.266 kb on - strand at 262.266 kb on - strand at 262.267 kb on + strand at 262.267 kb on + strand at 262.267 kb on + strand at 262.267 kb on + strand at 262.267 kb on + strand at 262.268 kb on - strand at 262.268 kb on - strand at 262.268 kb on - strand at 262.293 kb on - strand, within AMB_RS01180 at 262.588 kb on + strand, within AMB_RS01180 at 262.589 kb on - strand, within AMB_RS01180 at 262.589 kb on - strand, within AMB_RS01180 at 262.589 kb on - strand, within AMB_RS01180 at 262.589 kb on - strand, within AMB_RS01180 at 262.589 kb on - strand, within AMB_RS01180 at 262.702 kb on + strand at 262.703 kb on - strand at 262.726 kb on + strand at 262.727 kb on - strand at 262.727 kb on - strand at 262.727 kb on - strand at 262.774 kb on + strand at 262.775 kb on - strand at 262.775 kb on - strand at 262.817 kb on - strand at 262.946 kb on - strand, within AMB_RS01185 at 262.946 kb on - strand, within AMB_RS01185 at 263.135 kb on + strand, within AMB_RS01185 at 263.135 kb on + strand, within AMB_RS01185 at 263.135 kb on + strand, within AMB_RS01185 at 263.136 kb on - strand, within AMB_RS01185 at 263.136 kb on - strand, within AMB_RS01185 at 263.136 kb on - strand, within AMB_RS01185 at 263.209 kb on + strand, within AMB_RS01185 at 263.209 kb on + strand, within AMB_RS01185 at 263.210 kb on - strand, within AMB_RS01185 at 263.227 kb on + strand, within AMB_RS01185 at 263.596 kb on + strand, within AMB_RS01185 at 263.596 kb on + strand, within AMB_RS01185 at 263.597 kb on - strand, within AMB_RS01185 at 263.597 kb on - strand, within AMB_RS01185 at 263.597 kb on - strand, within AMB_RS01185 at 263.636 kb on + strand, within AMB_RS01185 at 263.637 kb on - strand, within AMB_RS01185 at 263.659 kb on + strand, within AMB_RS01185 at 263.659 kb on + strand, within AMB_RS01185 at 263.659 kb on + strand, within AMB_RS01185 at 263.695 kb on + strand, within AMB_RS01185 at 263.695 kb on + strand, within AMB_RS01185 at 263.695 kb on + strand, within AMB_RS01185 at 263.696 kb on - strand, within AMB_RS01185 at 263.696 kb on - strand, within AMB_RS01185 at 264.082 kb on + strand, within AMB_RS01185 at 264.178 kb on + strand, within AMB_RS01185 at 264.179 kb on - strand, within AMB_RS01185 at 264.179 kb on - strand, within AMB_RS01185 at 264.260 kb on + strand, within AMB_RS01185 at 264.260 kb on + strand, within AMB_RS01185 at 264.260 kb on + strand, within AMB_RS01185 at 264.261 kb on - strand, within AMB_RS01185 at 264.261 kb on - strand, within AMB_RS01185 at 264.261 kb on - strand, within AMB_RS01185 at 264.261 kb on - strand, within AMB_RS01185 at 264.261 kb on - strand, within AMB_RS01185 at 264.262 kb on + strand, within AMB_RS01185 at 264.262 kb on + strand, within AMB_RS01185 at 264.262 kb on + strand, within AMB_RS01185 at 264.262 kb on + strand, within AMB_RS01185 at 264.262 kb on + strand, within AMB_RS01185 at 264.263 kb on - strand, within AMB_RS01185 at 264.263 kb on - strand, within AMB_RS01185 at 264.263 kb on - strand, within AMB_RS01185 at 264.264 kb on + strand, within AMB_RS01185 at 264.264 kb on + strand, within AMB_RS01185 at 264.264 kb on + strand, within AMB_RS01185 at 264.294 kb on - strand, within AMB_RS01185 at 264.379 kb on + strand at 264.517 kb on - strand at 264.517 kb on - strand at 264.517 kb on - strand at 264.550 kb on + strand at 264.577 kb on + strand at 264.577 kb on + strand at 264.577 kb on + strand at 264.578 kb on - strand at 264.618 kb on - strand at 264.621 kb on + strand at 264.621 kb on + strand at 264.621 kb on + strand at 264.621 kb on + strand at 264.622 kb on - strand at 264.622 kb on - strand at 264.622 kb on - strand at 264.622 kb on - strand at 264.622 kb on - strand at 264.622 kb on - strand at 264.624 kb on + strand at 264.646 kb on + strand at 264.647 kb on - strand at 264.685 kb on + strand at 264.686 kb on - strand at 264.719 kb on + strand at 264.719 kb on + strand at 264.720 kb on - strand at 264.720 kb on - strand at 264.720 kb on - strand at 264.954 kb on - strand, within AMB_RS01190 at 265.253 kb on + strand, within AMB_RS01190 at 265.254 kb on - strand, within AMB_RS01190 at 265.272 kb on + strand, within AMB_RS01190 at 265.273 kb on - strand, within AMB_RS01190
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_mag_3 remove 261,871 + AMB_RS01175 0.72 +1.0 261,871 + AMB_RS01175 0.72 +0.1 261,871 + AMB_RS01175 0.72 -0.7 261,871 + AMB_RS01175 0.72 +1.5 261,871 + AMB_RS01175 0.72 -0.8 261,871 + AMB_RS01175 0.72 +0.5 261,872 - AMB_RS01175 0.72 -0.5 261,872 - AMB_RS01175 0.72 +0.4 261,872 - AMB_RS01175 0.72 -0.0 261,935 - AMB_RS01175 0.76 -1.9 261,973 + AMB_RS01175 0.79 -0.1 261,973 + AMB_RS01175 0.79 -0.9 261,974 - AMB_RS01175 0.79 -1.8 262,012 - AMB_RS01175 0.82 -2.9 262,181 - -0.3 262,181 - -0.0 262,240 + -1.1 262,241 - -1.1 262,241 - -2.1 262,241 - -1.5 262,241 - +0.3 262,241 - +0.1 262,265 + +0.2 262,266 - +0.2 262,266 - +0.2 262,267 + -1.5 262,267 + -0.3 262,267 + -2.1 262,267 + -1.7 262,267 + +0.2 262,268 - -0.1 262,268 - +0.5 262,268 - -0.5 262,293 - AMB_RS01180 0.13 -0.1 262,588 + AMB_RS01180 0.69 -1.1 262,589 - AMB_RS01180 0.69 -0.2 262,589 - AMB_RS01180 0.69 -0.5 262,589 - AMB_RS01180 0.69 -1.1 262,589 - AMB_RS01180 0.69 -0.4 262,589 - AMB_RS01180 0.69 +0.5 262,702 + -0.2 262,703 - -0.5 262,726 + -2.9 262,727 - -1.5 262,727 - +0.4 262,727 - -0.1 262,774 + +0.5 262,775 - -0.1 262,775 - +0.5 262,817 - +0.3 262,946 - AMB_RS01185 0.11 -2.8 262,946 - AMB_RS01185 0.11 -0.5 263,135 + AMB_RS01185 0.22 -0.2 263,135 + AMB_RS01185 0.22 -2.2 263,135 + AMB_RS01185 0.22 +0.3 263,136 - AMB_RS01185 0.22 -2.1 263,136 - AMB_RS01185 0.22 -0.9 263,136 - AMB_RS01185 0.22 -0.0 263,209 + AMB_RS01185 0.26 +0.8 263,209 + AMB_RS01185 0.26 +0.1 263,210 - AMB_RS01185 0.26 -1.9 263,227 + AMB_RS01185 0.27 -1.4 263,596 + AMB_RS01185 0.48 -1.7 263,596 + AMB_RS01185 0.48 +1.5 263,597 - AMB_RS01185 0.48 +0.4 263,597 - AMB_RS01185 0.48 -3.1 263,597 - AMB_RS01185 0.48 -1.9 263,636 + AMB_RS01185 0.50 -1.0 263,637 - AMB_RS01185 0.50 +0.9 263,659 + AMB_RS01185 0.51 -0.1 263,659 + AMB_RS01185 0.51 +1.5 263,659 + AMB_RS01185 0.51 +0.2 263,695 + AMB_RS01185 0.53 -0.9 263,695 + AMB_RS01185 0.53 -0.4 263,695 + AMB_RS01185 0.53 +0.5 263,696 - AMB_RS01185 0.53 -0.5 263,696 - AMB_RS01185 0.53 +0.5 264,082 + AMB_RS01185 0.75 +0.5 264,178 + AMB_RS01185 0.80 -2.2 264,179 - AMB_RS01185 0.80 +0.5 264,179 - AMB_RS01185 0.80 -0.1 264,260 + AMB_RS01185 0.85 -0.5 264,260 + AMB_RS01185 0.85 -0.8 264,260 + AMB_RS01185 0.85 -1.7 264,261 - AMB_RS01185 0.85 +0.6 264,261 - AMB_RS01185 0.85 +0.0 264,261 - AMB_RS01185 0.85 +0.1 264,261 - AMB_RS01185 0.85 -0.1 264,261 - AMB_RS01185 0.85 +0.2 264,262 + AMB_RS01185 0.85 +1.2 264,262 + AMB_RS01185 0.85 -1.1 264,262 + AMB_RS01185 0.85 -1.9 264,262 + AMB_RS01185 0.85 -1.4 264,262 + AMB_RS01185 0.85 -2.4 264,263 - AMB_RS01185 0.85 -0.2 264,263 - AMB_RS01185 0.85 -0.1 264,263 - AMB_RS01185 0.85 +0.5 264,264 + AMB_RS01185 0.85 -2.6 264,264 + AMB_RS01185 0.85 -0.9 264,264 + AMB_RS01185 0.85 +0.8 264,294 - AMB_RS01185 0.87 -1.4 264,379 + -2.5 264,517 - -0.1 264,517 - +0.3 264,517 - -2.4 264,550 + -0.5 264,577 + -1.4 264,577 + +0.3 264,577 + -1.4 264,578 - -0.9 264,618 - +0.1 264,621 + -1.9 264,621 + -2.0 264,621 + -0.2 264,621 + +0.9 264,622 - -1.1 264,622 - +0.2 264,622 - +0.3 264,622 - -0.2 264,622 - -1.9 264,622 - +1.5 264,624 + -0.6 264,646 + -1.7 264,647 - +0.0 264,685 + +1.0 264,686 - -4.6 264,719 + -2.4 264,719 + -2.2 264,720 - -1.4 264,720 - -2.8 264,720 - -4.0 264,954 - AMB_RS01190 0.15 -2.5 265,253 + AMB_RS01190 0.35 -1.7 265,254 - AMB_RS01190 0.35 -2.5 265,272 + AMB_RS01190 0.36 -3.4 265,273 - AMB_RS01190 0.37 -1.7
Or see this region's nucleotide sequence