Experiment: 30_an_mag_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01065 and AMB_RS01070 are separated by 151 nucleotides AMB_RS01070 and AMB_RS01075 are separated by 259 nucleotides
AMB_RS01065: AMB_RS01065 - formate hydrogenlyase, at 235,194 to 236,141
_RS01065
AMB_RS01070: AMB_RS01070 - hydrogenase 4 subunit B, at 236,293 to 238,302
_RS01070
AMB_RS01075: AMB_RS01075 - phosphoribosylamine--glycine ligase, at 238,562 to 239,836
_RS01075
Position (kb)
236
237
238
239 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 235.933 kb on + strand, within AMB_RS01065 at 235.934 kb on - strand, within AMB_RS01065 at 235.934 kb on - strand, within AMB_RS01065 at 235.999 kb on + strand, within AMB_RS01065 at 235.999 kb on + strand, within AMB_RS01065 at 235.999 kb on + strand, within AMB_RS01065 at 235.999 kb on + strand, within AMB_RS01065 at 235.999 kb on + strand, within AMB_RS01065 at 236.000 kb on - strand, within AMB_RS01065 at 236.000 kb on - strand, within AMB_RS01065 at 236.012 kb on - strand, within AMB_RS01065 at 236.360 kb on + strand at 236.360 kb on + strand at 236.361 kb on - strand at 236.361 kb on - strand at 236.361 kb on - strand at 236.585 kb on + strand, within AMB_RS01070 at 236.585 kb on + strand, within AMB_RS01070 at 236.585 kb on + strand, within AMB_RS01070 at 236.585 kb on + strand, within AMB_RS01070 at 236.680 kb on - strand, within AMB_RS01070 at 236.726 kb on + strand, within AMB_RS01070 at 236.727 kb on - strand, within AMB_RS01070 at 237.293 kb on + strand, within AMB_RS01070 at 237.294 kb on - strand, within AMB_RS01070 at 237.294 kb on - strand, within AMB_RS01070 at 237.294 kb on - strand, within AMB_RS01070 at 237.294 kb on - strand, within AMB_RS01070 at 237.444 kb on - strand, within AMB_RS01070 at 237.444 kb on - strand, within AMB_RS01070 at 237.556 kb on + strand, within AMB_RS01070 at 237.556 kb on + strand, within AMB_RS01070 at 237.557 kb on - strand, within AMB_RS01070 at 237.557 kb on - strand, within AMB_RS01070 at 237.557 kb on - strand, within AMB_RS01070 at 237.740 kb on + strand, within AMB_RS01070 at 237.740 kb on + strand, within AMB_RS01070 at 237.816 kb on - strand, within AMB_RS01070 at 237.912 kb on + strand, within AMB_RS01070 at 237.912 kb on + strand, within AMB_RS01070 at 237.912 kb on + strand, within AMB_RS01070 at 237.912 kb on + strand, within AMB_RS01070 at 237.912 kb on + strand, within AMB_RS01070 at 237.913 kb on - strand, within AMB_RS01070 at 237.913 kb on - strand, within AMB_RS01070 at 237.913 kb on - strand, within AMB_RS01070 at 237.913 kb on - strand, within AMB_RS01070 at 237.913 kb on - strand, within AMB_RS01070 at 237.965 kb on + strand, within AMB_RS01070 at 237.965 kb on + strand, within AMB_RS01070 at 237.966 kb on - strand, within AMB_RS01070 at 237.966 kb on - strand, within AMB_RS01070 at 237.966 kb on - strand, within AMB_RS01070 at 237.966 kb on - strand, within AMB_RS01070 at 238.019 kb on + strand, within AMB_RS01070 at 238.212 kb on - strand at 238.502 kb on + strand at 238.503 kb on - strand at 238.524 kb on + strand at 238.525 kb on - strand at 238.525 kb on - strand at 238.525 kb on - strand at 238.563 kb on + strand at 238.563 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_mag_3 remove 235,933 + AMB_RS01065 0.78 +1.1 235,934 - AMB_RS01065 0.78 -1.1 235,934 - AMB_RS01065 0.78 +0.1 235,999 + AMB_RS01065 0.85 -1.4 235,999 + AMB_RS01065 0.85 +0.2 235,999 + AMB_RS01065 0.85 -0.1 235,999 + AMB_RS01065 0.85 -1.6 235,999 + AMB_RS01065 0.85 +0.2 236,000 - AMB_RS01065 0.85 -0.9 236,000 - AMB_RS01065 0.85 -0.1 236,012 - AMB_RS01065 0.86 +0.2 236,360 + +1.0 236,360 + +0.2 236,361 - -0.4 236,361 - -0.1 236,361 - +1.5 236,585 + AMB_RS01070 0.15 -0.9 236,585 + AMB_RS01070 0.15 +0.2 236,585 + AMB_RS01070 0.15 -0.5 236,585 + AMB_RS01070 0.15 -2.2 236,680 - AMB_RS01070 0.19 +0.8 236,726 + AMB_RS01070 0.22 -1.5 236,727 - AMB_RS01070 0.22 -0.3 237,293 + AMB_RS01070 0.50 -1.9 237,294 - AMB_RS01070 0.50 -3.0 237,294 - AMB_RS01070 0.50 -0.9 237,294 - AMB_RS01070 0.50 +0.3 237,294 - AMB_RS01070 0.50 -4.7 237,444 - AMB_RS01070 0.57 -0.9 237,444 - AMB_RS01070 0.57 -1.0 237,556 + AMB_RS01070 0.63 +0.1 237,556 + AMB_RS01070 0.63 -0.1 237,557 - AMB_RS01070 0.63 -0.7 237,557 - AMB_RS01070 0.63 -0.1 237,557 - AMB_RS01070 0.63 -1.4 237,740 + AMB_RS01070 0.72 -0.9 237,740 + AMB_RS01070 0.72 -3.1 237,816 - AMB_RS01070 0.76 +0.1 237,912 + AMB_RS01070 0.81 -0.5 237,912 + AMB_RS01070 0.81 +0.1 237,912 + AMB_RS01070 0.81 +1.5 237,912 + AMB_RS01070 0.81 +0.9 237,912 + AMB_RS01070 0.81 -0.4 237,913 - AMB_RS01070 0.81 +0.2 237,913 - AMB_RS01070 0.81 +0.2 237,913 - AMB_RS01070 0.81 -1.4 237,913 - AMB_RS01070 0.81 -1.4 237,913 - AMB_RS01070 0.81 +0.4 237,965 + AMB_RS01070 0.83 -1.5 237,965 + AMB_RS01070 0.83 -0.9 237,966 - AMB_RS01070 0.83 +0.5 237,966 - AMB_RS01070 0.83 +0.5 237,966 - AMB_RS01070 0.83 -0.1 237,966 - AMB_RS01070 0.83 -1.4 238,019 + AMB_RS01070 0.86 -0.2 238,212 - -0.1 238,502 + -0.5 238,503 - +1.5 238,524 + +0.0 238,525 - -1.1 238,525 - -0.4 238,525 - +0.5 238,563 + -0.1 238,563 + -0.1
Or see this region's nucleotide sequence