Strain Fitness in Escherichia coli BW25113 around b1744

Experiment: Fusidic 0.03 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntspy and astE are separated by 329 nucleotidesastE and astB overlap by 8 nucleotides b1743: spy - envelope stress induced periplasmic protein (NCBI), at 1,823,164 to 1,823,649 spy b1744: astE - succinylglutamate desuccinylase (NCBI), at 1,823,979 to 1,824,947 astE b1745: astB - succinylarginine dihydrolase (NCBI), at 1,824,940 to 1,826,283 astB Position (kb) 1823 1824 1825Strain fitness (log2 ratio) -2 -1 0 1 2at 1823.108 kb on + strandat 1823.191 kb on - strandat 1823.293 kb on - strand, within spyat 1823.519 kb on + strand, within spyat 1823.549 kb on + strand, within spyat 1823.549 kb on + strand, within spyat 1823.559 kb on + strand, within spyat 1823.587 kb on + strand, within spyat 1823.595 kb on - strand, within spyat 1823.595 kb on - strand, within spyat 1823.613 kb on + strandat 1823.621 kb on - strandat 1823.629 kb on - strandat 1823.629 kb on - strandat 1823.683 kb on + strandat 1823.686 kb on + strandat 1823.703 kb on + strandat 1823.711 kb on - strandat 1823.729 kb on - strandat 1824.017 kb on - strandat 1824.017 kb on - strandat 1824.032 kb on + strandat 1824.047 kb on - strandat 1824.047 kb on - strandat 1824.052 kb on - strandat 1824.053 kb on - strandat 1824.059 kb on - strandat 1824.059 kb on - strandat 1824.087 kb on + strand, within astEat 1824.090 kb on - strand, within astEat 1824.090 kb on - strand, within astEat 1824.095 kb on - strand, within astEat 1824.095 kb on - strand, within astEat 1824.095 kb on - strand, within astEat 1824.095 kb on - strand, within astEat 1824.330 kb on + strand, within astEat 1824.333 kb on - strand, within astEat 1824.380 kb on + strand, within astEat 1824.397 kb on + strand, within astEat 1824.512 kb on + strand, within astEat 1824.650 kb on - strand, within astEat 1824.857 kb on + strandat 1824.871 kb on - strandat 1825.089 kb on - strand, within astBat 1825.285 kb on + strand, within astBat 1825.285 kb on + strand, within astBat 1825.293 kb on - strand, within astBat 1825.321 kb on - strand, within astBat 1825.321 kb on - strand, within astBat 1825.378 kb on + strand, within astBat 1825.727 kb on - strand, within astBat 1825.767 kb on - strand, within astB

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Per-strain Table

Position Strand Gene LocusTag Fraction Fusidic 0.03 mg/ml
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1,823,108 + -1.3
1,823,191 - -1.1
1,823,293 - spy b1743 0.27 +0.4
1,823,519 + spy b1743 0.73 +1.1
1,823,549 + spy b1743 0.79 -0.6
1,823,549 + spy b1743 0.79 +0.8
1,823,559 + spy b1743 0.81 +0.4
1,823,587 + spy b1743 0.87 +0.3
1,823,595 - spy b1743 0.89 -0.5
1,823,595 - spy b1743 0.89 -0.6
1,823,613 + +0.8
1,823,621 - +0.0
1,823,629 - -0.4
1,823,629 - -0.5
1,823,683 + -2.0
1,823,686 + +0.0
1,823,703 + +0.2
1,823,711 - +0.0
1,823,729 - -0.7
1,824,017 - -1.1
1,824,017 - +1.5
1,824,032 + +0.4
1,824,047 - -0.1
1,824,047 - -0.4
1,824,052 - +0.2
1,824,053 - +0.4
1,824,059 - +0.2
1,824,059 - -0.3
1,824,087 + astE b1744 0.11 +0.1
1,824,090 - astE b1744 0.11 +1.1
1,824,090 - astE b1744 0.11 -0.5
1,824,095 - astE b1744 0.12 +1.0
1,824,095 - astE b1744 0.12 -0.6
1,824,095 - astE b1744 0.12 -0.2
1,824,095 - astE b1744 0.12 -0.4
1,824,330 + astE b1744 0.36 +1.0
1,824,333 - astE b1744 0.37 -0.7
1,824,380 + astE b1744 0.41 +0.8
1,824,397 + astE b1744 0.43 +0.6
1,824,512 + astE b1744 0.55 +0.2
1,824,650 - astE b1744 0.69 -1.0
1,824,857 + -1.8
1,824,871 - +2.2
1,825,089 - astB b1745 0.11 +1.8
1,825,285 + astB b1745 0.26 -1.0
1,825,285 + astB b1745 0.26 +0.6
1,825,293 - astB b1745 0.26 +0.1
1,825,321 - astB b1745 0.28 -0.3
1,825,321 - astB b1745 0.28 -0.4
1,825,378 + astB b1745 0.33 +0.2
1,825,727 - astB b1745 0.59 +0.4
1,825,767 - astB b1745 0.62 -0.4

Or see this region's nucleotide sequence