Strain Fitness in Escherichia coli BW25113 around b0109

Experiment: Fusidic 0.03 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthofB and ppdD are separated by 9 nucleotidesppdD and nadC are separated by 202 nucleotidesnadC and ampD are separated by 87 nucleotidesampD and ampE overlap by 4 nucleotides b0107: hofB - conserved protein with nucleoside triphosphate hydrolase domain (NCBI), at 115,714 to 117,099 hofB b0108: ppdD - predicted major pilin subunit (NCBI), at 117,109 to 117,549 ppdD b0109: nadC - nicotinate-nucleotide pyrophosphorylase (NCBI), at 117,752 to 118,645 nadC b0110: ampD - N-acetyl-anhydromuranmyl-L-alanine amidase (NCBI), at 118,733 to 119,284 ampD b0111: ampE - predicted inner membrane protein (NCBI), at 119,281 to 120,135 ampE Position (kb) 117 118 119Strain fitness (log2 ratio) -2 -1 0 1 2at 116.760 kb on - strand, within hofBat 116.777 kb on + strand, within hofBat 116.777 kb on + strand, within hofBat 116.872 kb on + strand, within hofBat 116.882 kb on + strand, within hofBat 116.957 kb on - strand, within hofBat 116.964 kb on - strandat 117.046 kb on + strandat 117.056 kb on + strandat 117.069 kb on + strandat 117.075 kb on + strandat 117.075 kb on + strandat 117.075 kb on + strandat 117.081 kb on - strandat 117.081 kb on - strandat 117.084 kb on + strandat 117.087 kb on + strandat 117.227 kb on - strand, within ppdDat 117.281 kb on + strand, within ppdDat 117.281 kb on + strand, within ppdDat 117.293 kb on - strand, within ppdDat 117.302 kb on + strand, within ppdDat 117.302 kb on + strand, within ppdDat 117.385 kb on + strand, within ppdDat 117.387 kb on + strand, within ppdDat 117.508 kb on - strandat 117.508 kb on - strandat 117.511 kb on - strandat 117.511 kb on - strandat 117.525 kb on - strandat 117.525 kb on - strandat 117.603 kb on + strandat 117.603 kb on + strandat 117.839 kb on + strandat 117.856 kb on - strand, within nadCat 117.856 kb on - strand, within nadCat 117.879 kb on - strand, within nadCat 117.879 kb on - strand, within nadCat 117.881 kb on - strand, within nadCat 117.922 kb on - strand, within nadCat 117.922 kb on - strand, within nadCat 118.107 kb on - strand, within nadCat 118.201 kb on + strand, within nadCat 118.230 kb on + strand, within nadCat 118.230 kb on + strand, within nadCat 118.296 kb on + strand, within nadCat 118.355 kb on - strand, within nadCat 118.355 kb on - strand, within nadCat 118.364 kb on + strand, within nadCat 118.382 kb on - strand, within nadCat 118.538 kb on + strand, within nadCat 118.623 kb on + strandat 118.631 kb on - strandat 118.737 kb on + strandat 118.737 kb on + strandat 118.781 kb on + strandat 118.836 kb on + strand, within ampDat 118.868 kb on + strand, within ampDat 118.868 kb on + strand, within ampDat 118.904 kb on - strand, within ampDat 119.033 kb on - strand, within ampDat 119.101 kb on + strand, within ampDat 119.109 kb on - strand, within ampDat 119.109 kb on - strand, within ampDat 119.117 kb on - strand, within ampDat 119.119 kb on + strand, within ampDat 119.150 kb on - strand, within ampDat 119.150 kb on - strand, within ampDat 119.163 kb on + strand, within ampDat 119.168 kb on - strand, within ampDat 119.191 kb on + strand, within ampDat 119.191 kb on + strand, within ampDat 119.274 kb on + strandat 119.339 kb on + strandat 119.339 kb on + strandat 119.499 kb on - strand, within ampEat 119.499 kb on - strand, within ampEat 119.522 kb on + strand, within ampEat 119.528 kb on - strand, within ampEat 119.555 kb on - strand, within ampEat 119.555 kb on - strand, within ampEat 119.558 kb on - strand, within ampEat 119.567 kb on + strand, within ampEat 119.567 kb on + strand, within ampE

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Per-strain Table

Position Strand Gene LocusTag Fraction Fusidic 0.03 mg/ml
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116,760 - hofB b0107 0.75 +0.5
116,777 + hofB b0107 0.77 +0.4
116,777 + hofB b0107 0.77 +0.7
116,872 + hofB b0107 0.84 -0.2
116,882 + hofB b0107 0.84 +0.4
116,957 - hofB b0107 0.90 -0.1
116,964 - -0.2
117,046 + -0.7
117,056 + +0.2
117,069 + +0.7
117,075 + -1.2
117,075 + -1.0
117,075 + +0.2
117,081 - +1.1
117,081 - +1.5
117,084 + +1.1
117,087 + -0.2
117,227 - ppdD b0108 0.27 -0.3
117,281 + ppdD b0108 0.39 -0.7
117,281 + ppdD b0108 0.39 +0.4
117,293 - ppdD b0108 0.42 +0.0
117,302 + ppdD b0108 0.44 +0.5
117,302 + ppdD b0108 0.44 -0.2
117,385 + ppdD b0108 0.63 -0.3
117,387 + ppdD b0108 0.63 -1.0
117,508 - -0.2
117,508 - +0.8
117,511 - +0.5
117,511 - -0.5
117,525 - -0.2
117,525 - -0.2
117,603 + +0.5
117,603 + -1.6
117,839 + +0.4
117,856 - nadC b0109 0.12 +0.8
117,856 - nadC b0109 0.12 +0.4
117,879 - nadC b0109 0.14 -0.2
117,879 - nadC b0109 0.14 -0.0
117,881 - nadC b0109 0.14 +0.5
117,922 - nadC b0109 0.19 +0.4
117,922 - nadC b0109 0.19 +0.3
118,107 - nadC b0109 0.40 -0.9
118,201 + nadC b0109 0.50 +0.6
118,230 + nadC b0109 0.53 -0.0
118,230 + nadC b0109 0.53 +0.4
118,296 + nadC b0109 0.61 -0.7
118,355 - nadC b0109 0.67 +0.8
118,355 - nadC b0109 0.67 -2.5
118,364 + nadC b0109 0.68 -0.6
118,382 - nadC b0109 0.70 +0.3
118,538 + nadC b0109 0.88 +0.6
118,623 + +1.3
118,631 - -0.5
118,737 + -0.0
118,737 + +0.6
118,781 + +0.4
118,836 + ampD b0110 0.19 +0.2
118,868 + ampD b0110 0.24 +0.1
118,868 + ampD b0110 0.24 +1.8
118,904 - ampD b0110 0.31 -0.1
119,033 - ampD b0110 0.54 -0.2
119,101 + ampD b0110 0.67 +0.3
119,109 - ampD b0110 0.68 +0.5
119,109 - ampD b0110 0.68 -0.5
119,117 - ampD b0110 0.70 -0.5
119,119 + ampD b0110 0.70 -0.7
119,150 - ampD b0110 0.76 -0.2
119,150 - ampD b0110 0.76 -0.2
119,163 + ampD b0110 0.78 +1.0
119,168 - ampD b0110 0.79 +0.1
119,191 + ampD b0110 0.83 +0.2
119,191 + ampD b0110 0.83 +1.0
119,274 + -0.0
119,339 + -1.2
119,339 + +1.3
119,499 - ampE b0111 0.25 -1.6
119,499 - ampE b0111 0.25 +0.1
119,522 + ampE b0111 0.28 -1.4
119,528 - ampE b0111 0.29 +0.8
119,555 - ampE b0111 0.32 -0.2
119,555 - ampE b0111 0.32 -0.7
119,558 - ampE b0111 0.32 +0.8
119,567 + ampE b0111 0.33 -0.2
119,567 + ampE b0111 0.33 -0.5

Or see this region's nucleotide sequence