Experiment: 30_an_mag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS16245 and AMB_RS16250 overlap by 4 nucleotides AMB_RS16250 and AMB_RS16255 overlap by 4 nucleotides AMB_RS16255 and AMB_RS16260 overlap by 4 nucleotides AMB_RS16260 and AMB_RS16265 overlap by 1 nucleotides AMB_RS16265 and AMB_RS16270 are separated by 127 nucleotides
AMB_RS16245: AMB_RS16245 - Holliday junction branch migration DNA helicase RuvB, at 3,482,645 to 3,483,688
_RS16245
AMB_RS16250: AMB_RS16250 - hypothetical protein, at 3,483,685 to 3,483,972
_RS16250
AMB_RS16255: AMB_RS16255 - Holliday junction branch migration protein RuvA, at 3,483,969 to 3,484,607
_RS16255
AMB_RS16260: AMB_RS16260 - crossover junction endodeoxyribonuclease RuvC, at 3,484,604 to 3,485,104
_RS16260
AMB_RS16265: AMB_RS16265 - YebC/PmpR family DNA-binding transcriptional regulator, at 3,485,104 to 3,485,853
_RS16265
AMB_RS16270: AMB_RS16270 - organic solvent ABC transporter, at 3,485,981 to 3,486,601
_RS16270
Position (kb) 3484
3485
3486 Strain fitness (log2 ratio) -1
0
1 at 3483.747 kb on - strand, within AMB_RS16250 at 3483.747 kb on - strand, within AMB_RS16250 at 3483.872 kb on - strand, within AMB_RS16250 at 3485.268 kb on - strand, within AMB_RS16265 at 3485.900 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_mag_2 remove 3,483,747 - AMB_RS16250 0.22 +0.0 3,483,747 - AMB_RS16250 0.22 +0.2 3,483,872 - AMB_RS16250 0.65 -0.0 3,485,268 - AMB_RS16265 0.22 -0.9 3,485,900 - +0.0
Or see this region's nucleotide sequence