Experiment: 30_an_mag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS14295 and AMB_RS14300 are separated by 67 nucleotides AMB_RS14300 and AMB_RS14305 are separated by 36 nucleotides AMB_RS14305 and AMB_RS14310 are separated by 174 nucleotides AMB_RS14310 and AMB_RS14315 overlap by 26 nucleotides
AMB_RS14295: AMB_RS14295 - RNA degradosome polyphosphate kinase, at 3,038,525 to 3,040,663
_RS14295
AMB_RS14300: AMB_RS14300 - histidine phosphatase family protein, at 3,040,731 to 3,041,297
_RS14300
AMB_RS14305: AMB_RS14305 - tetratricopeptide repeat protein, at 3,041,334 to 3,043,520
_RS14305
AMB_RS14310: AMB_RS14310 - KR domain-containing protein, at 3,043,695 to 3,044,474
_RS14310
AMB_RS14315: AMB_RS14315 - DNA replication protein, at 3,044,449 to 3,045,144
_RS14315
Position (kb)
3041
3042
3043
3044 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3040.476 kb on + strand at 3040.476 kb on + strand at 3040.477 kb on - strand at 3040.477 kb on - strand at 3040.669 kb on - strand at 3040.688 kb on - strand at 3040.688 kb on - strand at 3040.706 kb on + strand at 3040.706 kb on + strand at 3040.707 kb on - strand at 3040.707 kb on - strand at 3040.707 kb on - strand at 3040.707 kb on - strand at 3040.709 kb on - strand at 3040.709 kb on - strand at 3040.709 kb on - strand at 3040.709 kb on - strand at 3040.711 kb on - strand at 3040.711 kb on - strand at 3040.711 kb on - strand at 3040.711 kb on - strand at 3040.869 kb on + strand, within AMB_RS14300 at 3040.869 kb on + strand, within AMB_RS14300 at 3040.869 kb on + strand, within AMB_RS14300 at 3040.869 kb on + strand, within AMB_RS14300 at 3040.869 kb on + strand, within AMB_RS14300 at 3040.869 kb on + strand, within AMB_RS14300 at 3040.869 kb on + strand, within AMB_RS14300 at 3040.869 kb on + strand, within AMB_RS14300 at 3040.870 kb on - strand, within AMB_RS14300 at 3040.870 kb on - strand, within AMB_RS14300 at 3040.870 kb on - strand, within AMB_RS14300 at 3040.870 kb on - strand, within AMB_RS14300 at 3040.995 kb on + strand, within AMB_RS14300 at 3040.995 kb on + strand, within AMB_RS14300 at 3040.996 kb on - strand, within AMB_RS14300 at 3041.293 kb on + strand at 3041.293 kb on + strand at 3041.293 kb on + strand at 3041.294 kb on - strand at 3041.295 kb on + strand at 3041.295 kb on + strand at 3041.295 kb on + strand at 3041.295 kb on + strand at 3041.296 kb on - strand at 3041.296 kb on - strand at 3041.296 kb on - strand at 3041.296 kb on - strand at 3041.461 kb on - strand at 3041.461 kb on - strand at 3041.461 kb on - strand at 3041.461 kb on - strand at 3041.461 kb on - strand at 3041.463 kb on + strand at 3041.463 kb on + strand at 3041.463 kb on + strand at 3041.464 kb on - strand at 3041.886 kb on + strand, within AMB_RS14305 at 3041.887 kb on - strand, within AMB_RS14305 at 3041.887 kb on - strand, within AMB_RS14305 at 3042.028 kb on - strand, within AMB_RS14305 at 3042.333 kb on + strand, within AMB_RS14305 at 3042.333 kb on + strand, within AMB_RS14305 at 3042.334 kb on - strand, within AMB_RS14305 at 3042.334 kb on - strand, within AMB_RS14305 at 3042.478 kb on - strand, within AMB_RS14305 at 3042.478 kb on - strand, within AMB_RS14305 at 3042.627 kb on + strand, within AMB_RS14305 at 3042.627 kb on + strand, within AMB_RS14305 at 3042.627 kb on + strand, within AMB_RS14305 at 3042.801 kb on + strand, within AMB_RS14305 at 3042.801 kb on + strand, within AMB_RS14305 at 3042.802 kb on - strand, within AMB_RS14305 at 3042.802 kb on - strand, within AMB_RS14305 at 3042.802 kb on - strand, within AMB_RS14305 at 3042.849 kb on + strand, within AMB_RS14305 at 3042.849 kb on + strand, within AMB_RS14305 at 3042.850 kb on - strand, within AMB_RS14305 at 3042.850 kb on - strand, within AMB_RS14305 at 3042.907 kb on + strand, within AMB_RS14305 at 3042.908 kb on - strand, within AMB_RS14305 at 3042.908 kb on - strand, within AMB_RS14305 at 3043.137 kb on + strand, within AMB_RS14305 at 3043.137 kb on + strand, within AMB_RS14305 at 3043.138 kb on - strand, within AMB_RS14305 at 3043.138 kb on - strand, within AMB_RS14305 at 3043.138 kb on - strand, within AMB_RS14305 at 3043.233 kb on + strand, within AMB_RS14305 at 3043.233 kb on + strand, within AMB_RS14305 at 3043.233 kb on + strand, within AMB_RS14305 at 3043.290 kb on + strand, within AMB_RS14305 at 3043.291 kb on - strand, within AMB_RS14305 at 3043.291 kb on - strand, within AMB_RS14305 at 3043.291 kb on - strand, within AMB_RS14305 at 3043.291 kb on - strand, within AMB_RS14305 at 3043.368 kb on + strand at 3043.368 kb on + strand at 3043.368 kb on + strand at 3043.368 kb on + strand at 3043.368 kb on + strand at 3043.368 kb on + strand at 3043.368 kb on + strand at 3043.368 kb on + strand at 3043.368 kb on + strand at 3043.368 kb on + strand at 3043.369 kb on - strand at 3043.369 kb on - strand at 3043.369 kb on - strand at 3043.369 kb on - strand at 3043.369 kb on - strand at 3043.369 kb on - strand at 3043.369 kb on - strand at 3043.369 kb on - strand at 3043.369 kb on - strand at 3043.369 kb on - strand at 3043.614 kb on - strand at 3043.774 kb on + strand, within AMB_RS14310 at 3043.774 kb on + strand, within AMB_RS14310 at 3043.774 kb on + strand, within AMB_RS14310 at 3043.775 kb on - strand, within AMB_RS14310 at 3044.060 kb on + strand, within AMB_RS14310 at 3044.060 kb on + strand, within AMB_RS14310 at 3044.060 kb on + strand, within AMB_RS14310 at 3044.060 kb on + strand, within AMB_RS14310 at 3044.060 kb on + strand, within AMB_RS14310 at 3044.060 kb on + strand, within AMB_RS14310 at 3044.061 kb on - strand, within AMB_RS14310 at 3044.061 kb on - strand, within AMB_RS14310 at 3044.061 kb on - strand, within AMB_RS14310 at 3044.061 kb on - strand, within AMB_RS14310 at 3044.061 kb on - strand, within AMB_RS14310
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_mag_2 remove 3,040,476 + -0.0 3,040,476 + +0.7 3,040,477 - -0.9 3,040,477 - -0.9 3,040,669 - -3.2 3,040,688 - -0.6 3,040,688 - -1.4 3,040,706 + +0.4 3,040,706 + -0.6 3,040,707 - +0.4 3,040,707 - +0.4 3,040,707 - -2.1 3,040,707 - -0.4 3,040,709 - +0.6 3,040,709 - -0.7 3,040,709 - -1.6 3,040,709 - -1.4 3,040,711 - -0.6 3,040,711 - -2.2 3,040,711 - -1.4 3,040,711 - -0.9 3,040,869 + AMB_RS14300 0.24 +0.6 3,040,869 + AMB_RS14300 0.24 +0.7 3,040,869 + AMB_RS14300 0.24 -2.1 3,040,869 + AMB_RS14300 0.24 +0.0 3,040,869 + AMB_RS14300 0.24 +0.8 3,040,869 + AMB_RS14300 0.24 -2.2 3,040,869 + AMB_RS14300 0.24 +1.4 3,040,869 + AMB_RS14300 0.24 +0.7 3,040,870 - AMB_RS14300 0.25 +0.2 3,040,870 - AMB_RS14300 0.25 -0.3 3,040,870 - AMB_RS14300 0.25 -0.3 3,040,870 - AMB_RS14300 0.25 -0.6 3,040,995 + AMB_RS14300 0.47 -1.8 3,040,995 + AMB_RS14300 0.47 +0.4 3,040,996 - AMB_RS14300 0.47 +0.0 3,041,293 + -2.4 3,041,293 + +0.1 3,041,293 + -2.2 3,041,294 - -1.2 3,041,295 + +0.1 3,041,295 + -0.9 3,041,295 + +0.1 3,041,295 + -0.2 3,041,296 - +1.4 3,041,296 - -0.5 3,041,296 - +0.4 3,041,296 - +0.5 3,041,461 - -0.9 3,041,461 - -0.6 3,041,461 - -1.6 3,041,461 - +1.4 3,041,461 - -0.2 3,041,463 + +0.0 3,041,463 + +0.1 3,041,463 + -0.6 3,041,464 - -1.8 3,041,886 + AMB_RS14305 0.25 +0.9 3,041,887 - AMB_RS14305 0.25 -2.3 3,041,887 - AMB_RS14305 0.25 -0.8 3,042,028 - AMB_RS14305 0.32 +0.6 3,042,333 + AMB_RS14305 0.46 -1.2 3,042,333 + AMB_RS14305 0.46 -0.3 3,042,334 - AMB_RS14305 0.46 +0.2 3,042,334 - AMB_RS14305 0.46 +0.4 3,042,478 - AMB_RS14305 0.52 -1.4 3,042,478 - AMB_RS14305 0.52 -0.6 3,042,627 + AMB_RS14305 0.59 +0.5 3,042,627 + AMB_RS14305 0.59 -0.3 3,042,627 + AMB_RS14305 0.59 -1.2 3,042,801 + AMB_RS14305 0.67 +0.5 3,042,801 + AMB_RS14305 0.67 -0.1 3,042,802 - AMB_RS14305 0.67 -0.2 3,042,802 - AMB_RS14305 0.67 +0.6 3,042,802 - AMB_RS14305 0.67 -0.3 3,042,849 + AMB_RS14305 0.69 -0.2 3,042,849 + AMB_RS14305 0.69 +0.8 3,042,850 - AMB_RS14305 0.69 +0.2 3,042,850 - AMB_RS14305 0.69 -0.3 3,042,907 + AMB_RS14305 0.72 +0.3 3,042,908 - AMB_RS14305 0.72 +0.2 3,042,908 - AMB_RS14305 0.72 -1.9 3,043,137 + AMB_RS14305 0.82 +0.4 3,043,137 + AMB_RS14305 0.82 +0.3 3,043,138 - AMB_RS14305 0.82 -0.1 3,043,138 - AMB_RS14305 0.82 -0.9 3,043,138 - AMB_RS14305 0.82 -0.2 3,043,233 + AMB_RS14305 0.87 +0.4 3,043,233 + AMB_RS14305 0.87 -1.8 3,043,233 + AMB_RS14305 0.87 -1.2 3,043,290 + AMB_RS14305 0.89 +0.1 3,043,291 - AMB_RS14305 0.89 -0.6 3,043,291 - AMB_RS14305 0.89 +0.0 3,043,291 - AMB_RS14305 0.89 +0.0 3,043,291 - AMB_RS14305 0.89 -1.2 3,043,368 + +0.6 3,043,368 + +0.4 3,043,368 + -0.2 3,043,368 + +0.1 3,043,368 + -0.9 3,043,368 + -0.3 3,043,368 + -0.1 3,043,368 + +0.3 3,043,368 + +0.7 3,043,368 + +0.4 3,043,369 - +0.3 3,043,369 - +0.1 3,043,369 - -0.8 3,043,369 - -0.1 3,043,369 - +0.5 3,043,369 - +0.3 3,043,369 - -0.2 3,043,369 - -0.1 3,043,369 - -0.9 3,043,369 - +0.4 3,043,614 - -2.5 3,043,774 + AMB_RS14310 0.10 +0.7 3,043,774 + AMB_RS14310 0.10 +0.4 3,043,774 + AMB_RS14310 0.10 +0.8 3,043,775 - AMB_RS14310 0.10 +0.1 3,044,060 + AMB_RS14310 0.47 -0.2 3,044,060 + AMB_RS14310 0.47 -0.0 3,044,060 + AMB_RS14310 0.47 -1.3 3,044,060 + AMB_RS14310 0.47 -2.8 3,044,060 + AMB_RS14310 0.47 -0.9 3,044,060 + AMB_RS14310 0.47 -0.2 3,044,061 - AMB_RS14310 0.47 -0.3 3,044,061 - AMB_RS14310 0.47 -1.6 3,044,061 - AMB_RS14310 0.47 -0.5 3,044,061 - AMB_RS14310 0.47 -2.1 3,044,061 - AMB_RS14310 0.47 -0.2
Or see this region's nucleotide sequence