Experiment: 30_an_mag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS03900 and AMB_RS03905 are separated by 102 nucleotides AMB_RS03905 and AMB_RS03910 overlap by 4 nucleotides AMB_RS03910 and AMB_RS03915 are separated by 221 nucleotides
AMB_RS03900: AMB_RS03900 - hypothetical protein, at 818,262 to 818,972
_RS03900
AMB_RS03905: AMB_RS03905 - ribonuclease H, at 819,075 to 819,539
_RS03905
AMB_RS03910: AMB_RS03910 - homoserine kinase, at 819,536 to 820,516
_RS03910
AMB_RS03915: AMB_RS03915 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 820,738 to 821,694
_RS03915
Position (kb) 819
820
821 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 818.673 kb on + strand, within AMB_RS03900 at 818.673 kb on + strand, within AMB_RS03900 at 818.674 kb on - strand, within AMB_RS03900 at 818.674 kb on - strand, within AMB_RS03900 at 818.674 kb on - strand, within AMB_RS03900 at 818.895 kb on + strand, within AMB_RS03900 at 818.895 kb on + strand, within AMB_RS03900 at 819.076 kb on + strand at 819.076 kb on + strand at 819.076 kb on + strand at 819.076 kb on + strand at 819.077 kb on - strand at 819.077 kb on - strand at 819.077 kb on - strand at 819.077 kb on - strand at 819.077 kb on - strand at 819.077 kb on - strand at 819.304 kb on + strand, within AMB_RS03905 at 819.304 kb on + strand, within AMB_RS03905 at 819.305 kb on - strand, within AMB_RS03905 at 819.305 kb on - strand, within AMB_RS03905 at 819.305 kb on - strand, within AMB_RS03905 at 819.319 kb on + strand, within AMB_RS03905 at 819.319 kb on + strand, within AMB_RS03905 at 819.499 kb on + strand at 819.499 kb on + strand at 819.499 kb on + strand at 819.500 kb on - strand at 819.500 kb on - strand at 819.500 kb on - strand at 820.560 kb on - strand at 820.560 kb on - strand at 820.560 kb on - strand at 820.678 kb on + strand at 820.679 kb on - strand at 820.739 kb on + strand at 820.739 kb on + strand at 820.739 kb on + strand at 820.740 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_mag_2 remove 818,673 + AMB_RS03900 0.58 -2.3 818,673 + AMB_RS03900 0.58 -0.5 818,674 - AMB_RS03900 0.58 -1.1 818,674 - AMB_RS03900 0.58 -0.1 818,674 - AMB_RS03900 0.58 -1.4 818,895 + AMB_RS03900 0.89 -1.6 818,895 + AMB_RS03900 0.89 +0.4 819,076 + -2.6 819,076 + -0.6 819,076 + -0.9 819,076 + +0.6 819,077 - +0.4 819,077 - +0.1 819,077 - -2.3 819,077 - -2.5 819,077 - -0.9 819,077 - -0.3 819,304 + AMB_RS03905 0.49 -2.8 819,304 + AMB_RS03905 0.49 -0.1 819,305 - AMB_RS03905 0.49 -0.6 819,305 - AMB_RS03905 0.49 -0.9 819,305 - AMB_RS03905 0.49 -1.9 819,319 + AMB_RS03905 0.52 +0.2 819,319 + AMB_RS03905 0.52 -0.3 819,499 + -0.1 819,499 + +0.4 819,499 + -0.9 819,500 - -0.1 819,500 - -0.3 819,500 - +1.4 820,560 - -0.6 820,560 - +0.0 820,560 - -1.4 820,678 + +0.3 820,679 - -0.9 820,739 + +0.4 820,739 + +0.2 820,739 + -0.9 820,740 - +0.4
Or see this region's nucleotide sequence