Experiment: 30_an_mag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS03020 and AMB_RS03025 are separated by 436 nucleotides AMB_RS03025 and AMB_RS03030 overlap by 23 nucleotides AMB_RS03030 and AMB_RS03035 are separated by 307 nucleotides
AMB_RS03020: AMB_RS03020 - 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ, at 631,887 to 632,738
_RS03020
AMB_RS03025: AMB_RS03025 - PAS domain-containing protein, at 633,175 to 633,699
_RS03025
AMB_RS03030: AMB_RS03030 - sensory rhodopsin II transducer, at 633,677 to 634,987
_RS03030
AMB_RS03035: AMB_RS03035 - aspartate-semialdehyde dehydrogenase, at 635,295 to 636,311
_RS03035
Position (kb) 633
634
635 Strain fitness (log2 ratio) -2
-1
0
1 at 632.719 kb on + strand at 632.719 kb on + strand at 632.720 kb on - strand at 632.720 kb on - strand at 632.731 kb on + strand at 632.779 kb on + strand at 632.779 kb on + strand at 632.780 kb on - strand at 632.780 kb on - strand at 632.823 kb on - strand at 632.825 kb on - strand at 632.871 kb on + strand at 632.875 kb on + strand at 632.876 kb on - strand at 632.899 kb on + strand at 632.944 kb on - strand at 632.944 kb on - strand at 632.949 kb on + strand at 632.950 kb on - strand at 632.950 kb on - strand at 632.950 kb on - strand at 632.950 kb on - strand at 632.950 kb on - strand at 632.950 kb on - strand at 633.028 kb on + strand at 633.029 kb on - strand at 633.064 kb on - strand at 633.088 kb on + strand at 633.140 kb on - strand at 633.193 kb on + strand at 633.194 kb on - strand at 633.194 kb on - strand at 633.214 kb on - strand at 633.271 kb on + strand, within AMB_RS03025 at 633.272 kb on - strand, within AMB_RS03025 at 633.272 kb on - strand, within AMB_RS03025 at 633.272 kb on - strand, within AMB_RS03025 at 633.421 kb on + strand, within AMB_RS03025 at 633.421 kb on + strand, within AMB_RS03025 at 633.422 kb on - strand, within AMB_RS03025 at 633.422 kb on - strand, within AMB_RS03025 at 633.422 kb on - strand, within AMB_RS03025 at 633.422 kb on - strand, within AMB_RS03025 at 633.425 kb on + strand, within AMB_RS03025 at 633.425 kb on + strand, within AMB_RS03025 at 633.425 kb on + strand, within AMB_RS03025 at 633.426 kb on - strand, within AMB_RS03025 at 633.426 kb on - strand, within AMB_RS03025 at 633.426 kb on - strand, within AMB_RS03025 at 633.426 kb on - strand, within AMB_RS03025 at 633.426 kb on - strand, within AMB_RS03025 at 633.426 kb on - strand, within AMB_RS03025 at 633.454 kb on + strand, within AMB_RS03025 at 633.455 kb on - strand, within AMB_RS03025 at 633.455 kb on - strand, within AMB_RS03025 at 633.501 kb on + strand, within AMB_RS03025 at 633.501 kb on + strand, within AMB_RS03025 at 633.511 kb on + strand, within AMB_RS03025 at 633.511 kb on + strand, within AMB_RS03025 at 633.511 kb on + strand, within AMB_RS03025 at 633.511 kb on + strand, within AMB_RS03025 at 633.512 kb on - strand, within AMB_RS03025 at 633.512 kb on - strand, within AMB_RS03025 at 633.569 kb on - strand, within AMB_RS03025 at 633.761 kb on - strand at 634.037 kb on + strand, within AMB_RS03030 at 634.038 kb on - strand, within AMB_RS03030 at 634.038 kb on - strand, within AMB_RS03030 at 634.049 kb on - strand, within AMB_RS03030 at 634.302 kb on - strand, within AMB_RS03030 at 634.302 kb on - strand, within AMB_RS03030 at 634.302 kb on - strand, within AMB_RS03030 at 634.655 kb on - strand, within AMB_RS03030 at 634.655 kb on - strand, within AMB_RS03030 at 635.016 kb on - strand at 635.016 kb on - strand at 635.053 kb on + strand at 635.053 kb on + strand at 635.146 kb on + strand at 635.146 kb on + strand at 635.149 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_mag_2 remove 632,719 + -0.1 632,719 + -2.1 632,720 - +0.3 632,720 - +0.6 632,731 + -0.7 632,779 + +0.4 632,779 + +0.1 632,780 - +0.1 632,780 - -1.3 632,823 - -0.2 632,825 - -2.6 632,871 + -0.7 632,875 + +0.5 632,876 - +0.2 632,899 + +0.3 632,944 - -0.6 632,944 - -0.5 632,949 + -2.6 632,950 - -0.7 632,950 - -0.2 632,950 - -0.3 632,950 - +0.5 632,950 - +1.5 632,950 - +0.3 633,028 + -0.1 633,029 - +0.5 633,064 - -0.4 633,088 + -0.5 633,140 - +0.9 633,193 + +0.8 633,194 - -1.1 633,194 - -0.8 633,214 - +0.4 633,271 + AMB_RS03025 0.18 -0.2 633,272 - AMB_RS03025 0.18 -0.1 633,272 - AMB_RS03025 0.18 -1.7 633,272 - AMB_RS03025 0.18 +0.2 633,421 + AMB_RS03025 0.47 +0.3 633,421 + AMB_RS03025 0.47 -1.7 633,422 - AMB_RS03025 0.47 +0.2 633,422 - AMB_RS03025 0.47 -2.0 633,422 - AMB_RS03025 0.47 -0.3 633,422 - AMB_RS03025 0.47 +1.2 633,425 + AMB_RS03025 0.48 -0.2 633,425 + AMB_RS03025 0.48 +0.1 633,425 + AMB_RS03025 0.48 -0.1 633,426 - AMB_RS03025 0.48 -0.1 633,426 - AMB_RS03025 0.48 -0.8 633,426 - AMB_RS03025 0.48 -0.1 633,426 - AMB_RS03025 0.48 +1.2 633,426 - AMB_RS03025 0.48 +0.6 633,426 - AMB_RS03025 0.48 -0.7 633,454 + AMB_RS03025 0.53 +0.0 633,455 - AMB_RS03025 0.53 -1.5 633,455 - AMB_RS03025 0.53 +0.6 633,501 + AMB_RS03025 0.62 +0.0 633,501 + AMB_RS03025 0.62 -2.0 633,511 + AMB_RS03025 0.64 -2.1 633,511 + AMB_RS03025 0.64 -1.6 633,511 + AMB_RS03025 0.64 -2.0 633,511 + AMB_RS03025 0.64 -2.0 633,512 - AMB_RS03025 0.64 -2.1 633,512 - AMB_RS03025 0.64 -0.5 633,569 - AMB_RS03025 0.75 -1.5 633,761 - -1.5 634,037 + AMB_RS03030 0.27 +0.1 634,038 - AMB_RS03030 0.28 -1.1 634,038 - AMB_RS03030 0.28 -0.7 634,049 - AMB_RS03030 0.28 -0.4 634,302 - AMB_RS03030 0.48 -0.1 634,302 - AMB_RS03030 0.48 +0.1 634,302 - AMB_RS03030 0.48 -1.3 634,655 - AMB_RS03030 0.75 -0.5 634,655 - AMB_RS03030 0.75 -2.0 635,016 - -0.9 635,016 - +0.7 635,053 + -0.1 635,053 + -0.1 635,146 + -0.3 635,146 + -0.2 635,149 + -0.5
Or see this region's nucleotide sequence