Experiment: 30_an_mag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS00600 and AMB_RS00605 overlap by 4 nucleotides AMB_RS00605 and AMB_RS00610 are separated by 140 nucleotides AMB_RS00610 and AMB_RS00615 overlap by 4 nucleotides
AMB_RS00600: AMB_RS00600 - asparagine synthase (glutamine-hydrolyzing), at 137,193 to 139,112
_RS00600
AMB_RS00605: AMB_RS00605 - hypothetical protein, at 139,109 to 140,809
_RS00605
AMB_RS00610: AMB_RS00610 - glucose-1-phosphate cytidylyltransferase, at 140,950 to 141,732
_RS00610
AMB_RS00615: AMB_RS00615 - nucleoside-diphosphate sugar epimerase, at 141,729 to 142,685
_RS00615
Position (kb)
139
140
141 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 138.131 kb on + strand, within AMB_RS00600 at 138.132 kb on - strand, within AMB_RS00600 at 138.132 kb on - strand, within AMB_RS00600 at 138.132 kb on - strand, within AMB_RS00600 at 138.345 kb on + strand, within AMB_RS00600 at 138.345 kb on + strand, within AMB_RS00600 at 138.420 kb on + strand, within AMB_RS00600 at 138.421 kb on - strand, within AMB_RS00600 at 138.421 kb on - strand, within AMB_RS00600 at 138.421 kb on - strand, within AMB_RS00600 at 138.654 kb on + strand, within AMB_RS00600 at 138.654 kb on + strand, within AMB_RS00600 at 138.654 kb on + strand, within AMB_RS00600 at 139.052 kb on - strand at 139.052 kb on - strand at 139.063 kb on - strand at 139.162 kb on - strand at 139.170 kb on - strand at 139.241 kb on + strand at 139.242 kb on - strand at 139.307 kb on + strand, within AMB_RS00605 at 139.411 kb on - strand, within AMB_RS00605 at 139.433 kb on + strand, within AMB_RS00605 at 139.433 kb on + strand, within AMB_RS00605 at 139.433 kb on + strand, within AMB_RS00605 at 139.707 kb on - strand, within AMB_RS00605 at 139.743 kb on - strand, within AMB_RS00605 at 139.743 kb on - strand, within AMB_RS00605 at 139.777 kb on + strand, within AMB_RS00605 at 139.778 kb on - strand, within AMB_RS00605 at 139.778 kb on - strand, within AMB_RS00605 at 139.784 kb on + strand, within AMB_RS00605 at 139.898 kb on + strand, within AMB_RS00605 at 139.898 kb on + strand, within AMB_RS00605 at 139.899 kb on - strand, within AMB_RS00605 at 139.899 kb on - strand, within AMB_RS00605 at 140.069 kb on + strand, within AMB_RS00605 at 140.069 kb on + strand, within AMB_RS00605 at 140.069 kb on + strand, within AMB_RS00605 at 140.070 kb on - strand, within AMB_RS00605 at 140.070 kb on - strand, within AMB_RS00605 at 140.070 kb on - strand, within AMB_RS00605 at 140.070 kb on - strand, within AMB_RS00605 at 140.070 kb on - strand, within AMB_RS00605 at 140.199 kb on - strand, within AMB_RS00605 at 140.200 kb on + strand, within AMB_RS00605 at 140.200 kb on + strand, within AMB_RS00605 at 140.200 kb on + strand, within AMB_RS00605 at 140.201 kb on - strand, within AMB_RS00605 at 140.201 kb on - strand, within AMB_RS00605 at 140.201 kb on - strand, within AMB_RS00605 at 140.239 kb on - strand, within AMB_RS00605 at 140.239 kb on - strand, within AMB_RS00605 at 140.283 kb on - strand, within AMB_RS00605 at 140.283 kb on - strand, within AMB_RS00605 at 140.346 kb on - strand, within AMB_RS00605 at 140.509 kb on + strand, within AMB_RS00605 at 140.517 kb on + strand, within AMB_RS00605 at 140.518 kb on - strand, within AMB_RS00605 at 140.546 kb on - strand, within AMB_RS00605 at 140.687 kb on + strand at 140.687 kb on + strand at 140.688 kb on - strand at 140.688 kb on - strand at 140.688 kb on - strand at 140.688 kb on - strand at 140.740 kb on - strand at 140.740 kb on - strand at 140.807 kb on + strand at 140.808 kb on - strand at 140.816 kb on + strand at 140.816 kb on + strand at 140.816 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_mag_2 remove 138,131 + AMB_RS00600 0.49 -0.4 138,132 - AMB_RS00600 0.49 -0.4 138,132 - AMB_RS00600 0.49 +0.4 138,132 - AMB_RS00600 0.49 +0.0 138,345 + AMB_RS00600 0.60 +0.6 138,345 + AMB_RS00600 0.60 +0.3 138,420 + AMB_RS00600 0.64 -0.0 138,421 - AMB_RS00600 0.64 -0.4 138,421 - AMB_RS00600 0.64 -2.2 138,421 - AMB_RS00600 0.64 +0.4 138,654 + AMB_RS00600 0.76 +0.0 138,654 + AMB_RS00600 0.76 -2.2 138,654 + AMB_RS00600 0.76 -0.3 139,052 - -0.0 139,052 - -0.8 139,063 - +0.1 139,162 - -0.4 139,170 - -2.4 139,241 + -2.3 139,242 - -0.4 139,307 + AMB_RS00605 0.12 -3.5 139,411 - AMB_RS00605 0.18 -1.0 139,433 + AMB_RS00605 0.19 +0.1 139,433 + AMB_RS00605 0.19 -1.6 139,433 + AMB_RS00605 0.19 -0.0 139,707 - AMB_RS00605 0.35 -1.2 139,743 - AMB_RS00605 0.37 -0.4 139,743 - AMB_RS00605 0.37 -3.5 139,777 + AMB_RS00605 0.39 -0.8 139,778 - AMB_RS00605 0.39 -2.7 139,778 - AMB_RS00605 0.39 -1.2 139,784 + AMB_RS00605 0.40 -1.6 139,898 + AMB_RS00605 0.46 -1.6 139,898 + AMB_RS00605 0.46 +0.2 139,899 - AMB_RS00605 0.46 -2.4 139,899 - AMB_RS00605 0.46 +0.6 140,069 + AMB_RS00605 0.56 +0.6 140,069 + AMB_RS00605 0.56 -1.6 140,069 + AMB_RS00605 0.56 +0.0 140,070 - AMB_RS00605 0.56 -1.4 140,070 - AMB_RS00605 0.56 -0.0 140,070 - AMB_RS00605 0.56 -0.4 140,070 - AMB_RS00605 0.56 +0.4 140,070 - AMB_RS00605 0.56 -0.0 140,199 - AMB_RS00605 0.64 -0.4 140,200 + AMB_RS00605 0.64 -2.4 140,200 + AMB_RS00605 0.64 +0.2 140,200 + AMB_RS00605 0.64 -0.6 140,201 - AMB_RS00605 0.64 +0.6 140,201 - AMB_RS00605 0.64 -1.6 140,201 - AMB_RS00605 0.64 +0.6 140,239 - AMB_RS00605 0.66 -1.0 140,239 - AMB_RS00605 0.66 -0.0 140,283 - AMB_RS00605 0.69 -1.0 140,283 - AMB_RS00605 0.69 -3.0 140,346 - AMB_RS00605 0.73 +0.2 140,509 + AMB_RS00605 0.82 -2.2 140,517 + AMB_RS00605 0.83 -0.0 140,518 - AMB_RS00605 0.83 +1.0 140,546 - AMB_RS00605 0.84 +1.6 140,687 + -2.7 140,687 + -1.0 140,688 - +0.3 140,688 - +1.6 140,688 - +0.4 140,688 - -0.2 140,740 - -2.6 140,740 - -3.0 140,807 + -1.4 140,808 - -2.6 140,816 + +0.5 140,816 + +2.1 140,816 + -0.3
Or see this region's nucleotide sequence