Strain Fitness in Escherichia coli BW25113 around b4444

Experiment: Bacitracin 1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntaas and rygA are separated by 87 nucleotidesrygA and omrB are separated by 114 nucleotidesomrB and galR are separated by 213 nucleotides b2836: aas - 2-acyl-glycerophospho-ethanolamine acyltransferase (NCBI), at 2,971,877 to 2,974,036 aas b4444: rygA - unknown RNA (NCBI), at 2,974,124 to 2,974,211 rygA b4445: omrB - sRNA down regulates OM proteins; positively regulated by OmpR/EnvZ; binds Hfq (RefSeq), at 2,974,326 to 2,974,407 omrB b2837: galR - DNA-binding transcriptional repressor (NCBI), at 2,974,621 to 2,975,652 galR Position (kb) 2974 2975Strain fitness (log2 ratio) -1 0 1 2 3at 2973.147 kb on + strand, within aasat 2973.200 kb on - strand, within aasat 2973.253 kb on + strand, within aasat 2973.261 kb on - strand, within aasat 2973.261 kb on - strand, within aasat 2973.351 kb on + strand, within aasat 2973.351 kb on + strand, within aasat 2973.354 kb on - strand, within aasat 2973.354 kb on - strand, within aasat 2973.526 kb on - strand, within aasat 2973.540 kb on - strand, within aasat 2973.699 kb on - strand, within aasat 2973.741 kb on + strand, within aasat 2973.754 kb on + strand, within aasat 2973.831 kb on - strandat 2973.831 kb on - strandat 2973.842 kb on - strandat 2973.894 kb on - strandat 2973.916 kb on + strandat 2973.918 kb on + strandat 2973.974 kb on - strandat 2973.995 kb on + strandat 2974.053 kb on - strandat 2974.055 kb on + strandat 2974.055 kb on + strandat 2974.090 kb on + strandat 2974.090 kb on + strandat 2974.097 kb on - strandat 2974.148 kb on + strand, within rygAat 2974.161 kb on - strand, within rygAat 2974.163 kb on - strand, within rygAat 2974.163 kb on - strand, within rygAat 2974.168 kb on - strand, within rygAat 2974.174 kb on - strand, within rygAat 2974.183 kb on - strand, within rygAat 2974.340 kb on + strand, within omrBat 2974.353 kb on - strand, within omrBat 2974.363 kb on - strand, within omrBat 2974.381 kb on - strand, within omrBat 2974.429 kb on - strandat 2974.581 kb on - strandat 2974.635 kb on + strandat 2974.635 kb on + strandat 2974.636 kb on - strandat 2974.636 kb on - strandat 2974.684 kb on + strandat 2974.684 kb on + strandat 2974.723 kb on + strandat 2974.733 kb on + strand, within galRat 2974.733 kb on + strand, within galRat 2974.741 kb on - strand, within galRat 2974.741 kb on - strand, within galRat 2974.751 kb on + strand, within galRat 2974.793 kb on + strand, within galRat 2974.793 kb on + strand, within galRat 2975.062 kb on - strand, within galRat 2975.089 kb on + strand, within galRat 2975.111 kb on - strand, within galRat 2975.111 kb on - strand, within galRat 2975.152 kb on - strand, within galRat 2975.168 kb on + strand, within galRat 2975.176 kb on - strand, within galR

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Per-strain Table

Position Strand Gene LocusTag Fraction Bacitracin 1 mg/ml
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2,973,147 + aas b2836 0.59 +0.6
2,973,200 - aas b2836 0.61 +3.3
2,973,253 + aas b2836 0.64 +0.0
2,973,261 - aas b2836 0.64 +0.9
2,973,261 - aas b2836 0.64 +0.3
2,973,351 + aas b2836 0.68 +1.2
2,973,351 + aas b2836 0.68 +0.7
2,973,354 - aas b2836 0.68 -1.7
2,973,354 - aas b2836 0.68 +0.3
2,973,526 - aas b2836 0.76 -0.7
2,973,540 - aas b2836 0.77 +2.4
2,973,699 - aas b2836 0.84 +0.2
2,973,741 + aas b2836 0.86 +0.4
2,973,754 + aas b2836 0.87 -0.3
2,973,831 - +1.2
2,973,831 - +0.5
2,973,842 - -1.5
2,973,894 - +0.1
2,973,916 + +0.5
2,973,918 + +1.6
2,973,974 - +0.6
2,973,995 + +0.5
2,974,053 - +1.0
2,974,055 + -0.3
2,974,055 + +0.3
2,974,090 + +1.0
2,974,090 + -0.7
2,974,097 - +0.3
2,974,148 + rygA b4444 0.27 -1.5
2,974,161 - rygA b4444 0.42 +0.3
2,974,163 - rygA b4444 0.44 +2.8
2,974,163 - rygA b4444 0.44 -1.3
2,974,168 - rygA b4444 0.50 +1.2
2,974,174 - rygA b4444 0.57 +0.7
2,974,183 - rygA b4444 0.67 +0.8
2,974,340 + omrB b4445 0.17 +0.7
2,974,353 - omrB b4445 0.33 +0.3
2,974,363 - omrB b4445 0.45 +0.1
2,974,381 - omrB b4445 0.67 -0.3
2,974,429 - +0.6
2,974,581 - -0.7
2,974,635 + +0.9
2,974,635 + +1.7
2,974,636 - -1.7
2,974,636 - -0.5
2,974,684 + +1.3
2,974,684 + +0.8
2,974,723 + +1.8
2,974,733 + galR b2837 0.11 -0.3
2,974,733 + galR b2837 0.11 -0.7
2,974,741 - galR b2837 0.12 -0.4
2,974,741 - galR b2837 0.12 -0.3
2,974,751 + galR b2837 0.13 +0.3
2,974,793 + galR b2837 0.17 +0.7
2,974,793 + galR b2837 0.17 -0.8
2,975,062 - galR b2837 0.43 +0.0
2,975,089 + galR b2837 0.45 -1.7
2,975,111 - galR b2837 0.47 -0.2
2,975,111 - galR b2837 0.47 +1.2
2,975,152 - galR b2837 0.51 +1.0
2,975,168 + galR b2837 0.53 -0.3
2,975,176 - galR b2837 0.54 +0.7

Or see this region's nucleotide sequence