Experiment: Bacitracin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nrfA and nrfB are separated by 44 nucleotides nrfB and nrfC overlap by 4 nucleotides nrfC and nrfD overlap by 4 nucleotides
b4070: nrfA - nitrite reductase, formate-dependent, cytochrome (NCBI), at 4,285,787 to 4,287,223
nrfA
b4071: nrfB - formate-dependent nitrite reductase; a penta-haeme cytochrome c (VIMSS), at 4,287,268 to 4,287,834
nrfB
b4072: nrfC - formate-dependent nitrite reductase, 4Fe4S subunit (NCBI), at 4,287,831 to 4,288,502
nrfC
b4073: nrfD - formate-dependent nitrite reductase, membrane subunit (NCBI), at 4,288,499 to 4,289,455
nrfD
Position (kb)
4287
4288
4289 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4286.846 kb on + strand, within nrfA at 4286.954 kb on + strand, within nrfA at 4286.962 kb on - strand, within nrfA at 4286.965 kb on - strand, within nrfA at 4286.965 kb on - strand, within nrfA at 4287.004 kb on + strand, within nrfA at 4287.058 kb on - strand, within nrfA at 4287.119 kb on + strand at 4287.149 kb on + strand at 4287.223 kb on + strand at 4287.250 kb on + strand at 4287.250 kb on + strand at 4287.250 kb on + strand at 4287.258 kb on - strand at 4287.262 kb on + strand at 4287.301 kb on + strand at 4287.301 kb on + strand at 4287.308 kb on + strand at 4287.313 kb on + strand at 4287.313 kb on + strand at 4287.328 kb on - strand, within nrfB at 4287.534 kb on - strand, within nrfB at 4287.560 kb on + strand, within nrfB at 4287.560 kb on + strand, within nrfB at 4287.582 kb on - strand, within nrfB at 4287.591 kb on + strand, within nrfB at 4287.619 kb on - strand, within nrfB at 4287.718 kb on + strand, within nrfB at 4287.823 kb on - strand at 4287.823 kb on - strand at 4287.836 kb on - strand at 4287.836 kb on - strand at 4287.905 kb on - strand, within nrfC at 4287.970 kb on - strand, within nrfC at 4287.981 kb on - strand, within nrfC at 4287.985 kb on + strand, within nrfC at 4288.125 kb on - strand, within nrfC at 4288.153 kb on - strand, within nrfC at 4288.182 kb on + strand, within nrfC at 4288.182 kb on + strand, within nrfC at 4288.212 kb on + strand, within nrfC at 4288.212 kb on + strand, within nrfC at 4288.220 kb on - strand, within nrfC at 4288.322 kb on + strand, within nrfC at 4288.458 kb on + strand at 4288.465 kb on + strand at 4288.465 kb on + strand at 4288.465 kb on + strand at 4288.465 kb on + strand at 4288.543 kb on - strand at 4288.617 kb on - strand, within nrfD at 4288.617 kb on - strand, within nrfD at 4288.650 kb on - strand, within nrfD at 4288.650 kb on - strand, within nrfD at 4288.655 kb on - strand, within nrfD at 4288.732 kb on + strand, within nrfD at 4288.740 kb on + strand, within nrfD at 4288.804 kb on + strand, within nrfD at 4288.804 kb on + strand, within nrfD at 4288.807 kb on + strand, within nrfD at 4288.825 kb on - strand, within nrfD at 4288.825 kb on - strand, within nrfD at 4288.888 kb on + strand, within nrfD at 4288.972 kb on + strand, within nrfD at 4288.972 kb on + strand, within nrfD at 4288.978 kb on + strand, within nrfD at 4288.978 kb on + strand, within nrfD at 4289.100 kb on - strand, within nrfD at 4289.154 kb on + strand, within nrfD at 4289.154 kb on + strand at 4289.163 kb on + strand, within nrfD at 4289.170 kb on + strand, within nrfD at 4289.170 kb on + strand, within nrfD at 4289.324 kb on + strand, within nrfD at 4289.324 kb on + strand, within nrfD at 4289.324 kb on + strand, within nrfD at 4289.361 kb on + strand at 4289.380 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 1 mg/ml remove 4,286,846 + nrfA b4070 0.74 +0.5 4,286,954 + nrfA b4070 0.81 +1.2 4,286,962 - nrfA b4070 0.82 +0.9 4,286,965 - nrfA b4070 0.82 +0.4 4,286,965 - nrfA b4070 0.82 +0.5 4,287,004 + nrfA b4070 0.85 +1.2 4,287,058 - nrfA b4070 0.88 -1.5 4,287,119 + +0.4 4,287,149 + -0.1 4,287,223 + +2.3 4,287,250 + -0.1 4,287,250 + -0.1 4,287,250 + -1.5 4,287,258 - -0.8 4,287,262 + +0.1 4,287,301 + +0.3 4,287,301 + +0.0 4,287,308 + +0.9 4,287,313 + -0.8 4,287,313 + +1.3 4,287,328 - nrfB b4071 0.11 +0.3 4,287,534 - nrfB b4071 0.47 -1.0 4,287,560 + nrfB b4071 0.51 -0.5 4,287,560 + nrfB b4071 0.51 +1.5 4,287,582 - nrfB b4071 0.55 -1.7 4,287,591 + nrfB b4071 0.57 +1.9 4,287,619 - nrfB b4071 0.62 +0.4 4,287,718 + nrfB b4071 0.79 +2.8 4,287,823 - -0.5 4,287,823 - +0.5 4,287,836 - +0.9 4,287,836 - -0.2 4,287,905 - nrfC b4072 0.11 +0.5 4,287,970 - nrfC b4072 0.21 +1.5 4,287,981 - nrfC b4072 0.22 +0.2 4,287,985 + nrfC b4072 0.23 +1.0 4,288,125 - nrfC b4072 0.44 +0.2 4,288,153 - nrfC b4072 0.48 +1.2 4,288,182 + nrfC b4072 0.52 +0.9 4,288,182 + nrfC b4072 0.52 -1.9 4,288,212 + nrfC b4072 0.57 +0.7 4,288,212 + nrfC b4072 0.57 +0.8 4,288,220 - nrfC b4072 0.58 -1.1 4,288,322 + nrfC b4072 0.73 -1.4 4,288,458 + -1.8 4,288,465 + -0.8 4,288,465 + +0.1 4,288,465 + -2.2 4,288,465 + +0.1 4,288,543 - -1.3 4,288,617 - nrfD b4073 0.12 +1.0 4,288,617 - nrfD b4073 0.12 -0.9 4,288,650 - nrfD b4073 0.16 +0.2 4,288,650 - nrfD b4073 0.16 +0.5 4,288,655 - nrfD b4073 0.16 +0.9 4,288,732 + nrfD b4073 0.24 +0.7 4,288,740 + nrfD b4073 0.25 +1.0 4,288,804 + nrfD b4073 0.32 +1.8 4,288,804 + nrfD b4073 0.32 +0.8 4,288,807 + nrfD b4073 0.32 +0.2 4,288,825 - nrfD b4073 0.34 -0.5 4,288,825 - nrfD b4073 0.34 -0.1 4,288,888 + nrfD b4073 0.41 +1.5 4,288,972 + nrfD b4073 0.49 -0.5 4,288,972 + nrfD b4073 0.49 +1.2 4,288,978 + nrfD b4073 0.50 +0.5 4,288,978 + nrfD b4073 0.50 -0.6 4,289,100 - nrfD b4073 0.63 +0.5 4,289,154 + nrfD b4073 0.68 +1.9 4,289,154 + +1.5 4,289,163 + nrfD b4073 0.69 -0.8 4,289,170 + nrfD b4073 0.70 -1.7 4,289,170 + nrfD b4073 0.70 +1.1 4,289,324 + nrfD b4073 0.86 +0.1 4,289,324 + nrfD b4073 0.86 -0.1 4,289,324 + nrfD b4073 0.86 -1.7 4,289,361 + +2.3 4,289,380 + +2.0
Or see this region's nucleotide sequence