Experiment: Bacitracin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dnaN and dnaA are separated by 4 nucleotides dnaA and rpmH are separated by 606 nucleotides rpmH and rnpA are separated by 16 nucleotides
b3701: dnaN - DNA polymerase III subunit beta (NCBI), at 3,879,244 to 3,880,344
dnaN
b3702: dnaA - chromosomal replication initiation protein (NCBI), at 3,880,349 to 3,881,752
dnaA
b3703: rpmH - 50S ribosomal protein L34 (NCBI), at 3,882,359 to 3,882,499
rpmH
b3704: rnpA - ribonuclease P (NCBI), at 3,882,516 to 3,882,875
rnpA
Position (kb)
3880
3881
3882 Strain fitness (log2 ratio)
-2
-1
0
1 at 3881.798 kb on - strand at 3881.847 kb on - strand at 3881.847 kb on - strand at 3881.884 kb on - strand at 3881.914 kb on + strand at 3881.932 kb on - strand at 3881.959 kb on + strand at 3881.972 kb on - strand at 3881.976 kb on + strand at 3882.000 kb on - strand at 3882.000 kb on - strand at 3882.009 kb on - strand at 3882.088 kb on + strand at 3882.097 kb on - strand at 3882.110 kb on + strand at 3882.110 kb on + strand at 3882.118 kb on - strand at 3882.118 kb on - strand at 3882.120 kb on - strand at 3882.163 kb on + strand at 3882.172 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 1 mg/ml remove 3,881,798 - +0.8 3,881,847 - -2.0 3,881,847 - +0.6 3,881,884 - -2.6 3,881,914 + +0.0 3,881,932 - -2.3 3,881,959 + -1.6 3,881,972 - -2.1 3,881,976 + +0.8 3,882,000 - +0.0 3,882,000 - +0.6 3,882,009 - -2.3 3,882,088 + +0.4 3,882,097 - -1.8 3,882,110 + -2.0 3,882,110 + -1.6 3,882,118 - +1.6 3,882,118 - -1.0 3,882,120 - -1.9 3,882,163 + -2.3 3,882,172 - -1.4
Or see this region's nucleotide sequence