Experiment: Bacitracin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt srmB and yfiE are separated by 208 nucleotides yfiE and yfiK are separated by 102 nucleotides yfiK and yfiD are separated by 55 nucleotides
b2576: srmB - ATP-dependent RNA helicase (NCBI), at 2,710,918 to 2,712,252
srmB
b2577: yfiE - putative transcriptional regulator LYSR-type (VIMSS), at 2,712,461 to 2,713,342
yfiE
b2578: yfiK - neutral amino-acid efflux system (NCBI), at 2,713,445 to 2,714,032
yfiK
b2579: yfiD - pyruvate formate lyase subunit (NCBI), at 2,714,088 to 2,714,471
yfiD
Position (kb)
2712
2713
2714 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2711.557 kb on - strand, within srmB at 2711.666 kb on + strand, within srmB at 2711.698 kb on + strand, within srmB at 2711.747 kb on + strand at 2711.849 kb on + strand, within srmB at 2711.857 kb on - strand, within srmB at 2711.857 kb on - strand, within srmB at 2711.927 kb on + strand, within srmB at 2712.112 kb on - strand, within srmB at 2712.203 kb on + strand at 2712.218 kb on + strand at 2712.226 kb on - strand at 2712.489 kb on - strand at 2712.491 kb on - strand at 2712.491 kb on - strand at 2712.491 kb on - strand at 2712.533 kb on - strand at 2712.558 kb on - strand, within yfiE at 2712.620 kb on + strand, within yfiE at 2712.620 kb on + strand, within yfiE at 2712.653 kb on - strand, within yfiE at 2712.653 kb on - strand, within yfiE at 2712.745 kb on - strand, within yfiE at 2712.948 kb on - strand, within yfiE at 2713.040 kb on - strand, within yfiE at 2713.074 kb on - strand, within yfiE at 2713.329 kb on - strand at 2713.412 kb on + strand at 2713.412 kb on + strand at 2713.500 kb on + strand at 2713.508 kb on - strand, within yfiK at 2713.508 kb on - strand, within yfiK at 2713.525 kb on + strand, within yfiK at 2713.525 kb on + strand, within yfiK at 2713.533 kb on + strand, within yfiK at 2713.663 kb on + strand, within yfiK at 2713.692 kb on - strand, within yfiK at 2713.761 kb on + strand, within yfiK at 2713.815 kb on + strand, within yfiK at 2713.815 kb on + strand, within yfiK at 2713.815 kb on + strand, within yfiK at 2713.815 kb on + strand, within yfiK at 2713.833 kb on + strand, within yfiK at 2713.833 kb on + strand, within yfiK at 2714.065 kb on - strand at 2714.072 kb on + strand at 2714.072 kb on + strand at 2714.093 kb on + strand at 2714.105 kb on + strand at 2714.129 kb on + strand, within yfiD at 2714.220 kb on + strand, within yfiD at 2714.220 kb on + strand, within yfiD at 2714.255 kb on + strand, within yfiD
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 1 mg/ml remove 2,711,557 - srmB b2576 0.48 -0.3 2,711,666 + srmB b2576 0.56 -1.3 2,711,698 + srmB b2576 0.58 -0.3 2,711,747 + +0.3 2,711,849 + srmB b2576 0.70 -0.7 2,711,857 - srmB b2576 0.70 +1.3 2,711,857 - srmB b2576 0.70 -1.5 2,711,927 + srmB b2576 0.76 -0.3 2,712,112 - srmB b2576 0.89 +0.3 2,712,203 + +0.1 2,712,218 + +1.6 2,712,226 - +0.1 2,712,489 - +1.9 2,712,491 - +0.3 2,712,491 - +0.7 2,712,491 - +0.0 2,712,533 - -0.4 2,712,558 - yfiE b2577 0.11 +1.2 2,712,620 + yfiE b2577 0.18 -1.0 2,712,620 + yfiE b2577 0.18 +0.2 2,712,653 - yfiE b2577 0.22 +1.7 2,712,653 - yfiE b2577 0.22 +2.7 2,712,745 - yfiE b2577 0.32 +1.3 2,712,948 - yfiE b2577 0.55 +0.7 2,713,040 - yfiE b2577 0.66 -0.7 2,713,074 - yfiE b2577 0.70 +0.9 2,713,329 - -1.5 2,713,412 + +0.8 2,713,412 + +0.3 2,713,500 + -1.8 2,713,508 - yfiK b2578 0.11 -2.0 2,713,508 - yfiK b2578 0.11 -1.8 2,713,525 + yfiK b2578 0.14 +0.4 2,713,525 + yfiK b2578 0.14 -1.0 2,713,533 + yfiK b2578 0.15 +0.2 2,713,663 + yfiK b2578 0.37 +0.3 2,713,692 - yfiK b2578 0.42 +1.0 2,713,761 + yfiK b2578 0.54 -0.4 2,713,815 + yfiK b2578 0.63 -0.3 2,713,815 + yfiK b2578 0.63 -0.8 2,713,815 + yfiK b2578 0.63 +0.7 2,713,815 + yfiK b2578 0.63 -0.2 2,713,833 + yfiK b2578 0.66 -0.7 2,713,833 + yfiK b2578 0.66 -2.9 2,714,065 - -0.6 2,714,072 + +0.8 2,714,072 + -1.8 2,714,093 + -0.5 2,714,105 + +0.6 2,714,129 + yfiD b2579 0.11 -0.1 2,714,220 + yfiD b2579 0.34 -0.7 2,714,220 + yfiD b2579 0.34 +0.6 2,714,255 + yfiD b2579 0.43 -1.0
Or see this region's nucleotide sequence